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Differential gene expression in nucleus accumbens somatostatin interneurons_cocaine_mice_pvalue
Description:
To characterize transcriptional alterations that cocaine induces in these cells, we perform cell type-specific RNA-sequencing on FACS-isolated nuclei of somatostatin interneurons and identified 1100 DETs enriched for processes related to neural plasticity. To profile the entire (non poly-A selected) transcriptome of NAc somatostatin interneurons, we generated a transgenic reporter line (SST-TLG498 mice) to label the nuclei of these cells with a modified form of EGFP that is retained in the nuclear membrane (EGFP-F)22, enabling their isolation from NAc dissections using FACS. We succeeded in FACS-isolating nuclei suitable for RNA-sequencing from individual SST-TLG498 mice. We proceeded with differential expression analysis of the RNA-sequencing data to identify differentially expressed transcripts (DETs) in NAc somatostatin interneurons in response to repeated cocaine exposure: 778 transcripts were upregulated by cocaine and 322 were downregulated.
Authors:
Efrain A Ribeiro, Marine Salery, Joseph R Scarpa, Erin S Calipari, Peter J Hamilton, Stacy M Ku, Hope Kronman, Immanuel Purushothaman, Barbara Juarez, Mitra Heshmati, Marie Doyle, Casey Lardner, Dominicka Burek, Ana Strat, Stephen Pirpinias, Ezekiell Mouzon, Ming-Hu Han, Rachael L Neve, Rosemary C Bagot, Andrew Kasarskis, Ja Wook Koo, Eric J Nestler
QTL associated with arthropathy in MRL and DBA/1 mice 2. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (57246565)
Authors:
Oishi H, Miyazaki T, Mizuki S, Kamogawa J, Lu LM, Tsubaki T, Arita N, Ono M, Yamamoto H, Nose M
QTL associated with inspiratory timing at baseline QTL 1. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (51563737)
Authors:
Tankersley CG, DiSilvestre DA, Jedlicka AE, Wilkins HM, Zhang L
QTL associated with experimental allergic encephalomyelitis susceptibility 20. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (28475226)
QTL associated with circulating hormone level QTL 2. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (56600603)
Authors:
Harper JM, Galecki AT, Burke DT, Pinkosky SL, Miller RA
QTL associated with insulin dependent diabetes susceptibility 3. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (60270627)
Authors:
Moritoki Y, Tsuda M, Tsuneyama K, Zhang W, Yoshida K, Lian ZX, Yang GX, Ridgway WM, Wicker LS, Ansari AA, Gershwin ME
QTL associated with novelty/stress induced locomotor activation 2. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (57246565)
QTL associated with phospholipid transfer protein activity QTL 1. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (69522098)
Authors:
Korstanje R, Albers JJ, Wolfbauer G, Li R, Tu AY, Churchill GA, Paigen BJ
QTL associated with suppressor of superoxide production. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (66391506)
Authors:
Mathews CE, Dunn BD, Hannigan MO, Huang CK, Leiter EH