Differential gene expression in nucleus accumbens somatostatin interneurons_cocaine_mice_pvalue
Description:
To characterize transcriptional alterations that cocaine induces in these cells, we perform cell type-specific RNA-sequencing on FACS-isolated nuclei of somatostatin interneurons and identified 1100 DETs enriched for processes related to neural plasticity. To profile the entire (non poly-A selected) transcriptome of NAc somatostatin interneurons, we generated a transgenic reporter line (SST-TLG498 mice) to label the nuclei of these cells with a modified form of EGFP that is retained in the nuclear membrane (EGFP-F)22, enabling their isolation from NAc dissections using FACS. We succeeded in FACS-isolating nuclei suitable for RNA-sequencing from individual SST-TLG498 mice. We proceeded with differential expression analysis of the RNA-sequencing data to identify differentially expressed transcripts (DETs) in NAc somatostatin interneurons in response to repeated cocaine exposure: 778 transcripts were upregulated by cocaine and 322 were downregulated.
Authors:
Efrain A Ribeiro, Marine Salery, Joseph R Scarpa, Erin S Calipari, Peter J Hamilton, Stacy M Ku, Hope Kronman, Immanuel Purushothaman, Barbara Juarez, Mitra Heshmati, Marie Doyle, Casey Lardner, Dominicka Burek, Ana Strat, Stephen Pirpinias, Ezekiell Mouzon, Ming-Hu Han, Rachael L Neve, Rosemary C Bagot, Andrew Kasarskis, Ja Wook Koo, Eric J Nestler
QTL Associated with Consumption level. On Chromosome 17 with a LOD score= 11.4, p-value =0.001. From a(n) intercross of QTL Associated with Consumption level. On Chromosome 20 with a LOD score= 8, p-value =0.001. From a(n) intercross of
Authors:
Marissal-Arvy N, Diane A, Moisan MP, Larue-Achagiotis C, Tridon C, Tome D, Fromentin G, Mormède P
QTL Associated with Consumption level. On Chromosome 12 with a LOD score= 2.1, p-value =0.68. From a(n) of QTL Associated with Alcohol response. On Chromosome 20 with a LOD score= 3.2, p-value =0.05. From a(n) intercoss of
Authors:
Radcliffe RA, Erwin VG, Bludeau P, Deng X, Fay T, Floyd KL, Deitrich RA
QTL Associated with blood insulin amount. On Chromosome 17 with a LOD score= 8.82, p-value =0.001. From a(n) intercross of QTL Associated with blood insulin amount. On Chromosome 20 with a LOD score= 6.38, p-value =0.001. From a(n) intercross of
Authors:
Marissal-Arvy N, Heliès JM, Tridon C, Moisan MP, Mormède P
Rotarod Baseline Chr# 11 rs3719581 (86772383) with right flanking marker rs13481061(62806119) and left marker rs13481161 (92322572). This was mapped in 300 + (b6x129)F2 mice.
Change in rotarod latency over training trials Chr#11 rs13481076(66532354) with right flanking marker rs3697686(58381052) and left marker rs3688955(90397849). This was mapped in 300 + (b6x129)F2 mice.
QTL Associated with Anti-type II collagen autoantibody titer. On Chromosome 20 with a LOD score= 30, p-value =0.001. From a(n) F2 of "DAxF344, DAxBN, BB/DRxBN"
Authors:
Griffiths MM, Wang J, Joe B, Dracheva S, Kawahito Y, Shepard JS, Reese VR, McCall-Vining S, Hashiramoto A, Cannon GW, Remmers EF, Wilder RL
QTL Associated with Prostate tumor susceptibility. On Chromosome 20 with a LOD score= 3.3, p-value =. From a(n) of
Authors:
Yamashita S, Suzuki S, Nomoto T, Kondo Y, Wakazono K, Tsujino Y, Sugimura T, Shirai T, Homma Y, Ushijima T
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