DESCRIPTION:

QTL Associated with Consumption level. On Chromosome 12 with a LOD score= 2.1, p-value =0.68. From a(n) of QTL Associated with Alcohol response. On Chromosome 20 with a LOD score= 3.2, p-value =0.05. From a(n) intercoss of

LABEL:

QTL-Alcrsp22-Rat-Chr 20

SCORE TYPE:

Binary

DATE ADDED:

2015-06-10

DATE UPDATED:

2020-05-06

SPECIES:

AUTHORS:

Radcliffe RA, Erwin VG, Bludeau P, Deng X, Fay T, Floyd KL, Deitrich RA

TITLE:

A major QTL for acute ethanol sensitivity in the alcohol tolerant and non-tolerant selected rat lines.

JOURNAL:

Genes, brain, and behavior None None, Vol 8, pp. 611-25

ABSTRACT:

1. Genes Brain Behav. 2009 Aug;8(6):611-25. doi: 10.1111/j.1601-183X.2009.00496.x. Epub 2009 Mar 23. A major QTL for acute ethanol sensitivity in the alcohol tolerant and non-tolerant selected rat lines. Radcliffe RA(1), Erwin VG, Bludeau P, Deng X, Fay T, Floyd KL, Deitrich RA. Author information: (1)Department of Pharmaceutical Sciences, University of Colorado Denver, Aurora, CO 80045, USA. richard.radcliffe@ucdenver.edu The Alcohol Tolerant and Alcohol Non-Tolerant rats (AT, ANT) were selectively bred for ethanol-induced ataxia as measured on the inclined plane. Here we report on a quantitative trait locus (QTL) study in an F(2) intercross population derived from inbred AT and ANT (IAT, IANT) and a follow-up study of congenics that were bred to examine one of the mapped QTLs. Over 1200 F(2) offspring were tested for inclined plane sensitivity, acute tolerance on the inclined plane, duration of the loss of righting reflex (LORR) and blood ethanol at regain of the righting reflex (BECRR). F(2) rats that were in the upper and lower 20% for inclined plane sensitivity were genotyped with 78 SSLP markers. Significant QTLs for inclined plane sensitivity were mapped on chromosomes 8 and 20; suggestive QTLs were mapped on chromosomes 1, 2 and 3. Highly significant QTLs for LORR duration (LOD = 12.4) and BECRR (LOD = 5.7) were mapped to the same locus on chromosome 1. Breeding and testing of reciprocal congenic lines confirmed the chromosome 1 LORR/BECRR QTL. A series of recombinant congenic sub-lines were bred to fine-map this QTL. Current results have narrowed the QTL to an interval of between 5 and 20 Mb. We expect to be able to narrow the interval to less than 5 Mb with additional genotyping and continued breeding of recombinant sub-congenic lines. PMCID: PMC2880637 PMID: 19500156 [PubMed - indexed for MEDLINE] PUBMED: 19500156
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