GeneSet Title
Tier III
Mouse
24 Genes
GS14916
LABEL: Morphine DependenceDESCRIPTION: Study subjected wild-type and mu opioid receptor knockout mice to an escalating morphine regimen to investigate whether overstimulation of mu opioid receptors impacts on lateral hypothalamus function. This gene set comprises the 29 mu opioid receptor-dependent genes found by the study to alter morphine expression in the lateral hypothalamus.
Tier III
Mouse
6 Genes
GS14917
LABEL: Morphine UpReg WildTypeDESCRIPTION: This gene set comprises the 7 lateral hypothalamus genes found by the study to upregulate gene expression in wild-type mice during chronic morphine treatment. Background: Study subjected wild-type and mu opioid receptor knockout mice to an escalating morphine regimen to investigate whether overstimulation of mu opiod receptors impacts on lateral hypothalamus function.
Tier III
Mouse
6 Genes
GS14918
LABEL: Morphine UpReg KnockoutDESCRIPTION: This gene set comprises the 7 lateral hypothalamus genes found by the study to upregulate gene expression mu opioid receptor knockout mice during chronic morphine treatment. Background: Study subjected wild-type and mu opioid receptor knockout mice to an escalating morphine regimen to investigate whether overstimulation of mu opiod receptors impacts on lateral hypothalamus function.
Tier III
Mouse
4 Genes
GS14919
LABEL: Morphine DwnReg WildTypeDESCRIPTION: This gene set comprises the 4 lateral hypothalamus genes found by the study to downregulate gene expression in wild-type mice during chronic morphine treatment. Background: Study subjected wild-type and mu opioid receptor knockout mice to an escalating morphine regimen to investigate whether overstimulation of mu opiod receptors impacts on lateral hypothalamus function.
Tier III
Mouse
4 Genes
GS14920
LABEL: Morphine DwnReg KnockoutDESCRIPTION: This gene set comprises the 4 lateral hypothalamus genes found by the study to downregulate gene expression mu opioid receptor knockout mice during chronic morphine treatment. Background: Study subjected wild-type and mu opioid receptor knockout mice to an escalating morphine regimen to investigate whether overstimulation of mu opiod receptors impacts on lateral hypothalamus function.
Tier I
Mouse
DRG
10 Genes
GS86394
LABEL: Chronic morphine - Morphine vs. SalineDESCRIPTION: Chronic morphine - Morphine vs. Saline Real-time quantitative polymerase chain reaction Change in gene expression Mice were injected twice daily with escalating doses of intraperitoneal morphine, beginning at 20 mg / kg and increasing to 100 mg / kg over 5 days. On day 6, only one injection of morphine was performed (100 mg / kg) and mice were then sacrificed for tissue collection 20 min after the last morphine injection. Control groups of mice were treated with saline under the same conditions. The 2-DeltaDeltaCt method was used to evaluate the differential expression level. A student t-test was performed to determine whether the fold change obtained for morphine-regulated genes is different from 1. We first tested gene expression levels using the same RNA samples as those used for the microarray hybridization experiment. We then measured transcript levels in samples from two independent chronic morphine treatments. Data were normalized using arbp reference gene. A second reference gene (beta-actin) was tested in each run as an internal control, and no change of expression was detected for either of these control genes. (NIF Table ID 430 [332])
Tier I
Mouse
DRG
10 Genes
GS86462
LABEL: Chronic morphine - Morphine vs. SalineDESCRIPTION: Chronic morphine - Morphine vs. Saline Real-time quantitative polymerase chain reaction Change in gene expression mu opioid Knockout (KO) mice Mice were injected twice daily with escalating doses of intraperitoneal morphine, beginning at 20 mg / kg and increasing to 100 mg / kg over 5 days. On day 6, only one injection of morphine was performed (100 mg / kg) and mice were then sacrificed for tissue collection 20 min after the last morphine injection. Control groups of mice were treated with saline under the same conditions. The 2-DeltaDeltaCt method was used to evaluate the differential expression level. A student t-test was performed to determine whether the fold change obtained for morphine-regulated genes is different from 1. Tested are gene expression levels using the same RNA samples as those used for the microarray hybridization experiment. Data were normalized using arbp reference gene. A second reference gene (beta-actin) was tested in each run as an internal control, and no change of expression was detected for either of these control genes. (NIF Table ID 430 [333])
Tier I
Mouse
DRG
26 Genes
GS86572
LABEL: Chronic morphine - Morphine vs. SalineDESCRIPTION: Chronic morphine - Morphine vs. Saline DNA microarray Change in gene expression Mice were injected twice daily with escalating doses of intraperitoneal morphine, beginning at 20 mg / kg and increasing to 100 mg / kg over 5 days. On day 6, only one injection of morphine was performed (100 mg / kg) and mice were then sacrificed for tissue collection 20 min after the last morphine injection. Control groups of mice were treated with saline under the same conditions. MAS 5.0 and Gene Chip operating GCOS v1 Affymetrix Softwares. (P values were obtained from Affymetrix MAS 5.0 software analysis.) Hierarchical clustering was performed using the Cluster and Treeview software. This table presents the list of probe sets that are selected as being regulated in the lateral hypothalamus of wild-type but not mu opioid receptor knockout mice. Data analysis was based on a 1.5-fold change morphine / saline threshold in at least two out of the three hybridization sets. (NIF Table ID 429 [331])
Tier I
Mouse
DRG
23 Genes
GS87495
LABEL: Chronic morphine - Morphine vs. SalineDESCRIPTION: Chronic morphine - Morphine vs. Saline DNA microarray Change in gene expression Mice were injected twice daily with escalating doses of intraperitoneal morphine, beginning at 20 mg / kg and increasing to 100 mg / kg over 5 days. On day 6, only one injection of morphine was performed (100 mg / kg) and mice were then sacrificed for tissue collection 20 min after the last morphine injection. Control groups of mice were treated with saline under the same conditions. MAS 5.0 and Gene Chip operating GCOS v1 Affymetrix Softwares. (P values were obtained from Affymetrix MAS 5.0 software analysis.) Hierarchical clustering was performed using the Cluster and Treeview software. This table presents the list of probe sets that are selected as being regulated in the lateral hypothalamus of wild-type but not mu opioid receptor knockout mice. Data analysis was based on a 1.5-fold change morphine / saline threshold in at least two out of the three hybridization sets. (NIF Method ID 331)
Tier I
Mouse
DRG
9 Genes
GS87496
LABEL: Chronic morphine - Morphine vs. SalineDESCRIPTION: Chronic morphine - Morphine vs. Saline Real-time quantitative polymerase chain reaction Change in gene expression Mice were injected twice daily with escalating doses of intraperitoneal morphine, beginning at 20 mg / kg and increasing to 100 mg / kg over 5 days. On day 6, only one injection of morphine was performed (100 mg / kg) and mice were then sacrificed for tissue collection 20 min after the last morphine injection. Control groups of mice were treated with saline under the same conditions. The 2-DeltaDeltaCt method was used to evaluate the differential expression level. A student t-test was performed to determine whether the fold change obtained for morphine-regulated genes is different from 1. We first tested gene expression levels using the same RNA samples as those used for the microarray hybridization experiment. We then measured transcript levels in samples from two independent chronic morphine treatments. Data were normalized using arbp reference gene. A second reference gene (beta-actin) was tested in each run as an internal control, and no change of expression was detected for either of these control genes. (NIF Method ID 332)
Tier I
Mouse
DRG
9 Genes
GS87497
LABEL: Chronic morphine - Morphine vs. SalineDESCRIPTION: Chronic morphine - Morphine vs. Saline Real-time quantitative polymerase chain reaction Change in gene expression Mice were injected twice daily with escalating doses of intraperitoneal morphine, beginning at 20 mg / kg and increasing to 100 mg / kg over 5 days. On day 6, only one injection of morphine was performed (100 mg / kg) and mice were then sacrificed for tissue collection 20 min after the last morphine injection. Control groups of mice were treated with saline under the same conditions. The 2-DeltaDeltaCt method was used to evaluate the differential expression level. A student t-test was performed to determine whether the fold change obtained for morphine-regulated genes is different from 1. Tested are gene expression levels using the same RNA samples as those used for the microarray hybridization experiment. Data were normalized using arbp reference gene. A second reference gene (beta-actin) was tested in each run as an internal control, and no change of expression was detected for either of these control genes. (NIF Method ID 333)
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