DESCRIPTION:
Chronic morphine - Morphine vs. Saline Real-time quantitative polymerase chain reaction Change in gene expression Mice were injected twice daily with escalating doses of intraperitoneal morphine, beginning at 20 mg / kg and increasing to 100 mg / kg over 5 days. On day 6, only one injection of morphine was performed (100 mg / kg) and mice were then sacrificed for tissue collection 20 min after the last morphine injection. Control groups of mice were treated with saline under the same conditions. The 2-DeltaDeltaCt method was used to evaluate the differential expression level. A student t-test was performed to determine whether the fold change obtained for morphine-regulated genes is different from 1. We first tested gene expression levels using the same RNA samples as those used for the microarray hybridization experiment. We then measured transcript levels in samples from two independent chronic morphine treatments. Data were normalized using arbp reference gene. A second reference gene (beta-actin) was tested in each run as an internal control, and no change of expression was detected for either of these control genes. (NIF Method ID 332)
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Chronic morphine - Morphine vs. Saline
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P-Value
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