Differential gene expression in nucleus accumbens somatostatin interneurons_cocaine_mice_pvalue
Description:
To characterize transcriptional alterations that cocaine induces in these cells, we perform cell type-specific RNA-sequencing on FACS-isolated nuclei of somatostatin interneurons and identified 1100 DETs enriched for processes related to neural plasticity. To profile the entire (non poly-A selected) transcriptome of NAc somatostatin interneurons, we generated a transgenic reporter line (SST-TLG498 mice) to label the nuclei of these cells with a modified form of EGFP that is retained in the nuclear membrane (EGFP-F)22, enabling their isolation from NAc dissections using FACS. We succeeded in FACS-isolating nuclei suitable for RNA-sequencing from individual SST-TLG498 mice. We proceeded with differential expression analysis of the RNA-sequencing data to identify differentially expressed transcripts (DETs) in NAc somatostatin interneurons in response to repeated cocaine exposure: 778 transcripts were upregulated by cocaine and 322 were downregulated.
Authors:
Efrain A Ribeiro, Marine Salery, Joseph R Scarpa, Erin S Calipari, Peter J Hamilton, Stacy M Ku, Hope Kronman, Immanuel Purushothaman, Barbara Juarez, Mitra Heshmati, Marie Doyle, Casey Lardner, Dominicka Burek, Ana Strat, Stephen Pirpinias, Ezekiell Mouzon, Ming-Hu Han, Rachael L Neve, Rosemary C Bagot, Andrew Kasarskis, Ja Wook Koo, Eric J Nestler
QTL associated with vertebral trabecular bone trait 3. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (100423186)
Authors:
Bouxsein ML, Uchiyama T, Rosen CJ, Shultz KL, Donahue LR, Turner CH, Sen S, Churchill GA, Mller R, Beamer WG
QTL associated with hepatocarcinogen resistance 1. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (106785035)
QTL associated with plasma apolipoprotein B (human) regulator 2. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (88709736)
Authors:
Ko C, Lee TL, Lau PW, Li J, Davis BT, Voyiaziakis E, Allison DB, Chua SC Jr, Huang LS
QTL associated with immune response to AAV2 QTL 1. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (59415112)
Authors:
Zhang HG, High KA, Wu Q, Yang P, Schlachterman A, Yu S, Yi N, Hsu HC, Mountz JD
QTL associated with modifier of mammary tumor growth 1. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (63026328)
Authors:
Le Voyer T, Rouse J, Lu Z, Lifsted T, Williams M, Hunter KW
QTL associated with anti-dsDNA antibody production in NZM 1. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (100072102)
Authors:
Waters ST, Fu SM, Gaskin F, Deshmukh US, Sung SS, Kannapell CC, Tung KS, McEwen SB, McDuffie M
QTL associated with B.burgdorferi-associated arthritis 23. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (102596575)
Authors:
Roper RJ, Weis JJ, McCracken BA, Green CB, Ma Y, Weber KS, Fairbairn D, Butterfield RJ, Potter MR, Zachary JF, Doerge RW, Teuscher C
QTL associated with CD4 virgin T cell subset 1. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (102244246)
QTL associated with circulating hormone level QTL 4. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (102244246)
Authors:
Harper JM, Galecki AT, Burke DT, Pinkosky SL, Miller RA
QTL associated with induction of brown adipocytes 7. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (100072102)
GeneWeaver