BECs at LORR Recovery Chr# 9 rs13480854(7524005) with right flanking marker mCV23893269 (4062079) and left marker rs3663313 (123063108). This was mapped in 300 + (b6x129)F2 mice.
Ethanol Induced Ataxia Chr#9 rs3655717(65312971) with right flanking marker rs13480112(26413932) and left marker rs13480421(111761261). This was mapped in 300 + (b6x129)F2 mice.
The current study used two inbred mouse strains, C57BL/6 J and A/J, to investigate the genetics of behavioral responses to fentanyl. Mice were tested for conditioned place preference and fentanyl-induced locomotor activity. C57BL/6J mice formed a conditioned place preference to fentanyl injections and fentanyl increased their activity. Neither effect was noted in A/J mice. We conducted RNA-sequencing on the nucleus accumbens of mice used for fentanyl-induced locomotor activity. Surprisingly, we noted few differentially expressed genes using treatment as the main factor. However many genes differed between strains.
Authors:
Samuel J Harp, Mariangela Martini, Will Rosenow, Larry D Mesner, Hugh Johnson, Charles R Farber, Emilie F Rissman
The current study used two inbred mouse strains, C57BL/6 J and A/J, to investigate the genetics of behavioral responses to fentanyl. Mice were tested for conditioned place preference and fentanyl-induced locomotor activity. C57BL/6J mice formed a conditioned place preference to fentanyl injections and fentanyl increased their activity. Neither effect was noted in A/J mice. We conducted RNA-sequencing on the nucleus accumbens of mice used for fentanyl-induced locomotor activity. Surprisingly, we noted few differentially expressed genes using treatment as the main factor. However many genes differed between strains.
Authors:
Samuel J Harp, Mariangela Martini, Will Rosenow, Larry D Mesner, Hugh Johnson, Charles R Farber, Emilie F Rissman
The current study used two inbred mouse strains, C57BL/6 J and A/J, to investigate the genetics of behavioral responses to fentanyl. Mice were tested for conditioned place preference and fentanyl-induced locomotor activity. C57BL/6J mice formed a conditioned place preference to fentanyl injections and fentanyl increased their activity. Neither effect was noted in A/J mice. We conducted RNA-sequencing on the nucleus accumbens of mice used for fentanyl-induced locomotor activity. Surprisingly, we noted few differentially expressed genes using treatment as the main factor. However many genes differed between strains.
Authors:
Samuel J Harp, Mariangela Martini, Will Rosenow, Larry D Mesner, Hugh Johnson, Charles R Farber, Emilie F Rissman
Analysis using RNA-seq of FACS-purified oligodendrocytes revealed a large cohort of morphine-regulated genes. In addition, to investigate cell-type-specific opioid responses, we performed single-cell RNA sequencing (scRNA-seq) of the nucleus accumbens of mice following acute morphine treatment. Differential expression analysis uncovered unique morphine-dependent transcriptional responses by oligodendrocytes and astrocytes.
Authors:
Denis Avey, Sumithra Sankararaman, Aldrin K Y Yim, Ruteja Barve, Jeffrey Milbrandt, Robi D Mitra
DEG mouse VTA 24hr withdrawal cocaine vs saline SA_pvalue
Description:
To determine how a history of cocaine self-administration (SA) influences circuit-wide transcriptomes, RNA-seq was performed on PFC, dorsal striatum (DStr), NAc, basolateral amygdala (BLA), ventral hippocampus (vHIP), and VTA, obtained from the following six groups of male C57BL/6J mice (Figure 1A): saline SA + 24 hr withdrawal (WD) (S24, n=5–8); cocaine SA + 24 hr WD (C24, n=5–8); saline SA + 30 d WD + saline re-exposure (SS, n=5–8); saline SA + 30 d WD + cocaine exposure (SC, n=5–8); cocaine SA + 30 d WD + saline exposure (CS, n=3–7); and cocaine SA + 30 d WD + cocaine re-exposure (CC, n=5–7). Genes presensted here are from the cocaine or saline SA + 24hr withdrawal paradigm for each brain region.
Authors:
Deena M Walker, Hannah M Cates, Yong-Hwee E Loh, Immanuel Purushothaman, Aarthi Ramakrishnan, Kelly M Cahill, Casey K Lardner, Arthur Godino, Hope G Kronman, Jacqui Rabkin, Zachary S Lorsch, Philipp Mews, Marie A Doyle, Jian Feng, Benoit Labonté, Ja Wook Koo, Rosemary C Bagot, Ryan W Logan, Marianne L Seney, Erin S Calipari, Li Shen, Eric J Nestler
To determine how a history of cocaine self-administration (SA) influences circuit-wide transcriptomes, RNA-seq was performed on PFC, dorsal striatum (DStr), NAc, basolateral amygdala (BLA), ventral hippocampus (vHIP), and VTA, obtained from the following six groups of male C57BL/6J mice (Figure 1A): saline SA + 24 hr withdrawal (WD) (S24, n=5–8); cocaine SA + 24 hr WD (C24, n=5–8); saline SA + 30 d WD + saline re-exposure (SS, n=5–8); saline SA + 30 d WD + cocaine exposure (SC, n=5–8); cocaine SA + 30 d WD + saline exposure (CS, n=3–7); and cocaine SA + 30 d WD + cocaine re-exposure (CC, n=5–7). Genes presensted here are from the cocaine SA + 30 day withdrawal followed by saline exposure (CS) paradigm versus saline SA + 30 day withdrawal followed by saline re-exposure (SS), for each brain region.
Authors:
Deena M Walker, Hannah M Cates, Yong-Hwee E Loh, Immanuel Purushothaman, Aarthi Ramakrishnan, Kelly M Cahill, Casey K Lardner, Arthur Godino, Hope G Kronman, Jacqui Rabkin, Zachary S Lorsch, Philipp Mews, Marie A Doyle, Jian Feng, Benoit Labonté, Ja Wook Koo, Rosemary C Bagot, Ryan W Logan, Marianne L Seney, Erin S Calipari, Li Shen, Eric J Nestler
Gene expression mouse BLA pattern A C24 vs S24_pvalue
Description:
RNA-seq was performed on PFC, dorsal striatum (DStr), NAc, basolateral amygdala (BLA), ventral hippocampus (vHIP), and VTA, obtained from the following six groups of male C57BL/6J mice (Figure 1A): saline SA + 24 hr withdrawal (WD) (S24, n=5–8); cocaine SA + 24 hr WD (C24, n=5–8); saline SA + 30 d WD + saline re-exposure (SS, n=5–8); saline SA + 30 d WD + cocaine exposure (SC, n=5–8); cocaine SA + 30 d WD + saline exposure (CS, n=3–7); and cocaine SA + 30 d WD + cocaine re-exposure (CC, n=5–7). To focus on genes that were uniquely altered following context/drug re-exposure after WD, we compared all groups to the same baseline (S24). We focused on three patterns associated with drug use: first-ever exposure to cocaine (SC; Pattern A; Figure 2B), re-exposure to cocaine-paired context (CS, Pattern B, Figure 2C), and re-exposure to cocaine-paired context + cocaine (CC, Pattern C, Figure 2D). Each Pattern includes genes that were both differentially expressed from S24 (p<0.05; fold change>15%) and distinct from all other groups. The pattern A genes were significantly differentially expressed (see above) between the saline SA with cocaine exposure after 30 days and baseline (i.e. SC vs S4). Differential expression of the pattern A genes are presented for each group (C24 vs S24, SS vs S4, SC vs S4, CS vs S4, and CC vs S4) and each brain region.
Authors:
Deena M Walker, Hannah M Cates, Yong-Hwee E Loh, Immanuel Purushothaman, Aarthi Ramakrishnan, Kelly M Cahill, Casey K Lardner, Arthur Godino, Hope G Kronman, Jacqui Rabkin, Zachary S Lorsch, Philipp Mews, Marie A Doyle, Jian Feng, Benoit Labonté, Ja Wook Koo, Rosemary C Bagot, Ryan W Logan, Marianne L Seney, Erin S Calipari, Li Shen, Eric J Nestler
Gene expression mouse BLA pattern A SS vs S24_pvalue
Description:
RNA-seq was performed on PFC, dorsal striatum (DStr), NAc, basolateral amygdala (BLA), ventral hippocampus (vHIP), and VTA, obtained from the following six groups of male C57BL/6J mice (Figure 1A): saline SA + 24 hr withdrawal (WD) (S24, n=5–8); cocaine SA + 24 hr WD (C24, n=5–8); saline SA + 30 d WD + saline re-exposure (SS, n=5–8); saline SA + 30 d WD + cocaine exposure (SC, n=5–8); cocaine SA + 30 d WD + saline exposure (CS, n=3–7); and cocaine SA + 30 d WD + cocaine re-exposure (CC, n=5–7). To focus on genes that were uniquely altered following context/drug re-exposure after WD, we compared all groups to the same baseline (S24). We focused on three patterns associated with drug use: first-ever exposure to cocaine (SC; Pattern A; Figure 2B), re-exposure to cocaine-paired context (CS, Pattern B, Figure 2C), and re-exposure to cocaine-paired context + cocaine (CC, Pattern C, Figure 2D). Each Pattern includes genes that were both differentially expressed from S24 (p<0.05; fold change>15%) and distinct from all other groups. The pattern A genes were significantly differentially expressed (see above) between the saline SA with cocaine exposure after 30 days and baseline (i.e. SC vs S4). Differential expression of the pattern A genes are presented for each group (C24 vs S24, SS vs S4, SC vs S4, CS vs S4, and CC vs S4) and each brain region.
Authors:
Deena M Walker, Hannah M Cates, Yong-Hwee E Loh, Immanuel Purushothaman, Aarthi Ramakrishnan, Kelly M Cahill, Casey K Lardner, Arthur Godino, Hope G Kronman, Jacqui Rabkin, Zachary S Lorsch, Philipp Mews, Marie A Doyle, Jian Feng, Benoit Labonté, Ja Wook Koo, Rosemary C Bagot, Ryan W Logan, Marianne L Seney, Erin S Calipari, Li Shen, Eric J Nestler
Gene expression mouse BLA pattern A SC vs S24_pvalue
Description:
RNA-seq was performed on PFC, dorsal striatum (DStr), NAc, basolateral amygdala (BLA), ventral hippocampus (vHIP), and VTA, obtained from the following six groups of male C57BL/6J mice (Figure 1A): saline SA + 24 hr withdrawal (WD) (S24, n=5–8); cocaine SA + 24 hr WD (C24, n=5–8); saline SA + 30 d WD + saline re-exposure (SS, n=5–8); saline SA + 30 d WD + cocaine exposure (SC, n=5–8); cocaine SA + 30 d WD + saline exposure (CS, n=3–7); and cocaine SA + 30 d WD + cocaine re-exposure (CC, n=5–7). To focus on genes that were uniquely altered following context/drug re-exposure after WD, we compared all groups to the same baseline (S24). We focused on three patterns associated with drug use: first-ever exposure to cocaine (SC; Pattern A; Figure 2B), re-exposure to cocaine-paired context (CS, Pattern B, Figure 2C), and re-exposure to cocaine-paired context + cocaine (CC, Pattern C, Figure 2D). Each Pattern includes genes that were both differentially expressed from S24 (p<0.05; fold change>15%) and distinct from all other groups. The pattern A genes were significantly differentially expressed (see above) between the saline SA with cocaine exposure after 30 days and baseline (i.e. SC vs S4). Differential expression of the pattern A genes are presented for each group (C24 vs S24, SS vs S4, SC vs S4, CS vs S4, and CC vs S4) and each brain region.
Authors:
Deena M Walker, Hannah M Cates, Yong-Hwee E Loh, Immanuel Purushothaman, Aarthi Ramakrishnan, Kelly M Cahill, Casey K Lardner, Arthur Godino, Hope G Kronman, Jacqui Rabkin, Zachary S Lorsch, Philipp Mews, Marie A Doyle, Jian Feng, Benoit Labonté, Ja Wook Koo, Rosemary C Bagot, Ryan W Logan, Marianne L Seney, Erin S Calipari, Li Shen, Eric J Nestler
Gene expression mouse BLA pattern A CS vs S24_pvalue
Description:
RNA-seq was performed on PFC, dorsal striatum (DStr), NAc, basolateral amygdala (BLA), ventral hippocampus (vHIP), and VTA, obtained from the following six groups of male C57BL/6J mice (Figure 1A): saline SA + 24 hr withdrawal (WD) (S24, n=5–8); cocaine SA + 24 hr WD (C24, n=5–8); saline SA + 30 d WD + saline re-exposure (SS, n=5–8); saline SA + 30 d WD + cocaine exposure (SC, n=5–8); cocaine SA + 30 d WD + saline exposure (CS, n=3–7); and cocaine SA + 30 d WD + cocaine re-exposure (CC, n=5–7). To focus on genes that were uniquely altered following context/drug re-exposure after WD, we compared all groups to the same baseline (S24). We focused on three patterns associated with drug use: first-ever exposure to cocaine (SC; Pattern A; Figure 2B), re-exposure to cocaine-paired context (CS, Pattern B, Figure 2C), and re-exposure to cocaine-paired context + cocaine (CC, Pattern C, Figure 2D). Each Pattern includes genes that were both differentially expressed from S24 (p<0.05; fold change>15%) and distinct from all other groups. The pattern A genes were significantly differentially expressed (see above) between the saline SA with cocaine exposure after 30 days and baseline (i.e. SC vs S4). Differential expression of the pattern A genes are presented for each group (C24 vs S24, SS vs S4, SC vs S4, CS vs S4, and CC vs S4) and each brain region.
Authors:
Deena M Walker, Hannah M Cates, Yong-Hwee E Loh, Immanuel Purushothaman, Aarthi Ramakrishnan, Kelly M Cahill, Casey K Lardner, Arthur Godino, Hope G Kronman, Jacqui Rabkin, Zachary S Lorsch, Philipp Mews, Marie A Doyle, Jian Feng, Benoit Labonté, Ja Wook Koo, Rosemary C Bagot, Ryan W Logan, Marianne L Seney, Erin S Calipari, Li Shen, Eric J Nestler
Gene expression mouse BLA pattern A CC vs S24_pvalue
Description:
RNA-seq was performed on PFC, dorsal striatum (DStr), NAc, basolateral amygdala (BLA), ventral hippocampus (vHIP), and VTA, obtained from the following six groups of male C57BL/6J mice (Figure 1A): saline SA + 24 hr withdrawal (WD) (S24, n=5–8); cocaine SA + 24 hr WD (C24, n=5–8); saline SA + 30 d WD + saline re-exposure (SS, n=5–8); saline SA + 30 d WD + cocaine exposure (SC, n=5–8); cocaine SA + 30 d WD + saline exposure (CS, n=3–7); and cocaine SA + 30 d WD + cocaine re-exposure (CC, n=5–7). To focus on genes that were uniquely altered following context/drug re-exposure after WD, we compared all groups to the same baseline (S24). We focused on three patterns associated with drug use: first-ever exposure to cocaine (SC; Pattern A; Figure 2B), re-exposure to cocaine-paired context (CS, Pattern B, Figure 2C), and re-exposure to cocaine-paired context + cocaine (CC, Pattern C, Figure 2D). Each Pattern includes genes that were both differentially expressed from S24 (p<0.05; fold change>15%) and distinct from all other groups. The pattern A genes were significantly differentially expressed (see above) between the saline SA with cocaine exposure after 30 days and baseline (i.e. SC vs S4). Differential expression of the pattern A genes are presented for each group (C24 vs S24, SS vs S4, SC vs S4, CS vs S4, and CC vs S4) and each brain region.
Authors:
Deena M Walker, Hannah M Cates, Yong-Hwee E Loh, Immanuel Purushothaman, Aarthi Ramakrishnan, Kelly M Cahill, Casey K Lardner, Arthur Godino, Hope G Kronman, Jacqui Rabkin, Zachary S Lorsch, Philipp Mews, Marie A Doyle, Jian Feng, Benoit Labonté, Ja Wook Koo, Rosemary C Bagot, Ryan W Logan, Marianne L Seney, Erin S Calipari, Li Shen, Eric J Nestler
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