GeneSet Details :: GeneWeaver

Tier IV GS409874 • Differentially expressed genes in mouse ventral tegmental area (VTA) cocaine vs saline self-administration + 24hrs withdrawal_pvalue

DESCRIPTION:

To determine how a history of cocaine self-administration (SA) influences circuit-wide transcriptomes, RNA-seq was performed on PFC, dorsal striatum (DStr), NAc, basolateral amygdala (BLA), ventral hippocampus (vHIP), and VTA, obtained from the following six groups of male C57BL/6J mice (Figure 1A): saline SA + 24 hr withdrawal (WD) (S24, n=5–8); cocaine SA + 24 hr WD (C24, n=5–8); saline SA + 30 d WD + saline re-exposure (SS, n=5–8); saline SA + 30 d WD + cocaine exposure (SC, n=5–8); cocaine SA + 30 d WD + saline exposure (CS, n=3–7); and cocaine SA + 30 d WD + cocaine re-exposure (CC, n=5–7). Genes presensted here are from the cocaine or saline SA + 24hr withdrawal paradigm for each brain region.

LABEL:

DEG mouse VTA 24hr withdrawal cocaine vs saline SA_pvalue

SCORE TYPE:

P-Value

THRESHOLD:

<= 0.5

GENES IN THRESHOLD:

255

DATE ADDED:

2025-01-06

DATE UPDATED:

2025-01-10

SPECIES:

AUTHORS:

Deena M Walker, Hannah M Cates, Yong-Hwee E Loh, Immanuel Purushothaman, Aarthi Ramakrishnan, Kelly M Cahill, Casey K Lardner, Arthur Godino, Hope G Kronman, Jacqui Rabkin, Zachary S Lorsch, Philipp Mews, Marie A Doyle, Jian Feng, Benoit Labonté, Ja Wook Koo, Rosemary C Bagot, Ryan W Logan, Marianne L Seney, Erin S Calipari, Li Shen, Eric J Nestler

TITLE:

Cocaine Self-administration Alters Transcriptome-wide Responses in the Brain's Reward Circuitry.

JOURNAL:

Biological psychiatry Dec 2018, Vol 84, pp. 867-880

ABSTRACT:

Background: Global changes in gene expression underlying circuit and behavioral dysregulation associated with cocaine addiction remain incompletely understood. Here, we show how a history of cocaine self-administration (SA) reprograms transcriptome-wide responses throughout the brain's reward circuitry at baseline and in response to context and/or cocaine re-exposure after prolonged withdrawal (WD). Methods: We assigned male mice to one of six groups: saline/cocaine SA + 24-hour WD or saline/cocaine SA + 30-day WD + an acute saline/cocaine challenge within the previous drug-paired context. RNA sequencing was conducted on six interconnected brain reward regions. Using pattern analysis of gene expression and factor analysis of behavior, we identified genes that are strongly associated with addiction-related behaviors and uniquely altered by a history of cocaine SA. We then identified potential upstream regulators of these genes. Results: We focused on three patterns of gene expression that reflect responses to 1) acute cocaine, 2) context re-exposure, and 3) drug + context re-exposure. These patterns revealed region-specific regulation of gene expression. Further analysis revealed that each of these gene expression patterns correlated with an addiction index-a composite score of several addiction-like behaviors during cocaine SA-in a region-specific manner. Cyclic adenosine monophosphate response element binding protein and nuclear receptor families were identified as key upstream regulators of genes associated with such behaviors. Conclusions: This comprehensive picture of transcriptome-wide regulation in the brain's reward circuitry by cocaine SA and prolonged WD provides new insight into the molecular basis of cocaine addiction, which will guide future studies of the key molecular pathways involved. PUBMED: 29861096
Find other GeneSets from this publication

Annotation Information

No sequence read archive data associated with this GeneSet.

Social Control, Formal (D012926)

Brain (D001921)

Gene Expression Regulation (D005786)

Family (D005190)

RNA (D012313)

Forecasting (D005544)

Behavior (D001519)

Indexes (D020481)

Ego (D004532)

Mice (D051379)

Carrier Proteins (D002352)

Adenosine (D000241)

Adenosine Monophosphate (D000249)

Gene Expression (D015870)

Proteins (D011506)

History (D006664)

Amygdala (D000679)

Pharmaceutical Preparations (D004364)

Cocaine-Related Disorders (D019970)

Receptors, Cytoplasmic and Nuclear (D018160)

Response Elements (D020218)

Genes (D005796)

Cocaine (D003042)

Sequence Analysis, RNA (D017423)

Factor Analysis, Statistical (D005163)

Methods (D008722)

Organization and Administration (D009934)

Elements (D004602)

Reward (D012201)

Hippocampus (D006624)

amygdala (MA:0000887)

striatum (MA:0000891)

dorsal striatum (MA:0002971)

brain (MA:0000168)

hippocampus (MA:0000191)

addiction (MP:0002555)

biological regulation (GO:0065007)

nascent polypeptide-associated complex (GO:0005854)

binding (GO:0005488)

gene expression (GO:0010467)

regulation of gene expression (GO:0010468)

behavior (GO:0007610)

Score or penalty (EDAM_data:1772)

RNA (EDAM_topic:0099)

Analysis (EDAM_operation:2945)

Genetics (EDAM_topic:3053)

Sequencing (EDAM_topic:3168)

protein (EDAM_format:1208)

Transcriptomics (EDAM_topic:0203)

Pathways, networks and models (EDAM_topic:0602)

RNA-Seq (EDAM_topic:3170)

rna (EDAM_format:1213)

N-acetyl-L-cysteine (CHEBI:28939)

(Z)-N-cyclopropyl-11-methyldodec-2-enamide (CHEBI:87143)

protein (CHEBI:36080)

adenosine (CHEBI:16335)

maleate(2-) (CHEBI:30780)

cocaine (CHEBI:27958)

cocaine(1+) (CHEBI:60056)

NAC (CHEBI:7421)

drug (CHEBI:23888)

protein polypeptide chain (CHEBI:16541)

ribonucleic acid (CHEBI:33697)

AMP (CHEBI:16027)

atom (CHEBI:33250)

perfluorinated compound (CHEBI:134091)

Acute (HP:0011009)

RNA-seq evidence (ECO:0000295)

C57BL/6J (EFO:0000606)

exposure (EFO:0000487)

C57BL (EFO:0005181)

C24 ecotype (EFO:0006987)

ventral (EFO:0001662)

obsolete_corpus striatum (EFO:0000381)

transcription profiling by high throughput sequencing (EFO:0002770)

saline (EFO:0002677)

dorsal (EFO:0001656)

cocaine dependence (EFO:0002610)

transcriptome (EFO:0004421)

RNA-seq assay (MMO:0000659)

amygdala (UBERON:0001876)

archicortex (UBERON:0002961)

brain (UBERON:0000955)

Ammon's horn (UBERON:0001954)

hippocampal formation (UBERON:0002421)

male organism (UBERON:0003101)

organ (UBERON:0000062)

ventral tegmental area (UBERON:0002691)

caudate-putamen (UBERON:0005383)

dorsal striatum (UBERON:0005382)

adult cerebral ganglion (UBERON:6110636)

striatum (UBERON:0002435)

basolateral amygdaloid nuclear complex (UBERON:0006107)

Gene List • 255 Genes

Genes in threshold: 255

Uploaded As	Gene Symbol Gene Symbol Entrez Ensembl Gene UniGene MGI FlyBase WormBase RGD ZFIN	Homology	Score	Priority	LinkOuts	Emphasis

GeneSet Information

Tier IV GS409874 • Differentially expressed genes in mouse ventral tegmental area (VTA) cocaine vs saline self-administration + 24hrs withdrawal_pvalue

Annotation Information

Gene List • 255 Genes

Manage GeneSets

Analyze GeneSets

Manage Accounts

GeneWeaver Links

Policies

What seems to be the problem?

Description:

Name:

Email:

GeneSet Information

Tier IV GS409874 • Differentially expressed genes in mouse ventral tegmental area (VTA) cocaine vs saline self-administration + 24hrs withdrawal_pvalue

Annotation Information

Gene List • 255 Genes

Add Selected GeneSets to Project(s)

The following GeneSet(s) will be added:

New Project Name

New Project Name:

Comments:

Share Selected GeneSets with Group(s)

The following GeneSet(s) will be added:

Delete GeneSet

Are you sure that you want to delete GeneSet ? This will permanently remove this GeneSet and may produce concurrency issues in projects.

Request Curation for Geneset

A request will be submitted to the Gene Weaver core curators for the following geneset:

Notes for the Curator (required):

Select Type of Data Export

GeneWeaver offers several export options. The 'GeneSet Complete' format can be used as an upload file in GeneWeaver's batch upload function.

Manage GeneSets

Analyze GeneSets

Manage Accounts

GeneWeaver Links

Policies