Differential gene expression in nucleus accumbens somatostatin interneurons_cocaine_mice_pvalue
Description:
To characterize transcriptional alterations that cocaine induces in these cells, we perform cell type-specific RNA-sequencing on FACS-isolated nuclei of somatostatin interneurons and identified 1100 DETs enriched for processes related to neural plasticity. To profile the entire (non poly-A selected) transcriptome of NAc somatostatin interneurons, we generated a transgenic reporter line (SST-TLG498 mice) to label the nuclei of these cells with a modified form of EGFP that is retained in the nuclear membrane (EGFP-F)22, enabling their isolation from NAc dissections using FACS. We succeeded in FACS-isolating nuclei suitable for RNA-sequencing from individual SST-TLG498 mice. We proceeded with differential expression analysis of the RNA-sequencing data to identify differentially expressed transcripts (DETs) in NAc somatostatin interneurons in response to repeated cocaine exposure: 778 transcripts were upregulated by cocaine and 322 were downregulated.
Authors:
Efrain A Ribeiro, Marine Salery, Joseph R Scarpa, Erin S Calipari, Peter J Hamilton, Stacy M Ku, Hope Kronman, Immanuel Purushothaman, Barbara Juarez, Mitra Heshmati, Marie Doyle, Casey Lardner, Dominicka Burek, Ana Strat, Stephen Pirpinias, Ezekiell Mouzon, Ming-Hu Han, Rachael L Neve, Rosemary C Bagot, Andrew Kasarskis, Ja Wook Koo, Eric J Nestler
Ethanol Induced Ataxia Chr#17 rs3672987(33247165) with right flanking marker rs4136382(3388912) and left marker rs3715723(58810428). This was mapped in 300 + (b6x129)F2 mice.
Genes with particular expression in the Orbital area, medial part, layer 1. Data represent fold expression difference in structure versus grey matter average expression.
Genes with particular expression in the Primary somatosensory area, layer 4. Data represent fold expression difference in structure versus grey matter average expression.
Genes with particular expression in the Nucleus ambiguus. Data represent fold expression difference in structure versus grey matter average expression.
Genes with particular expression in the Nucleus ambiguus, ventral division. Data represent fold expression difference in structure versus grey matter average expression.
Genes with particular expression in the Gustatory areas, layer 2/3. Data represent fold expression difference in structure versus grey matter average expression.
Genes with particular expression in the Anterior cingulate area, dorsal part, layer 1. Data represent fold expression difference in structure versus grey matter average expression.
Genes with particular expression in the Nucleus ambiguus, dorsal division. Data represent fold expression difference in structure versus grey matter average expression.
Genes with particular expression in the Medial geniculate complex, dorsal part. Data represent fold expression difference in structure versus grey matter average expression.
Genes with particular expression in the Superior olivary complex, lateral part. Data represent fold expression difference in structure versus grey matter average expression.
Genes with particular expression in the Superior olivary complex, periolivary region. Data represent fold expression difference in structure versus grey matter average expression.
Genes with particular expression in the Anterior olfactory nucleus, external part. Data represent fold expression difference in structure versus grey matter average expression.
Genes with particular expression in the Superior olivary complex. Data represent fold expression difference in structure versus grey matter average expression.
Genes with particular expression in the Retrosplenial area, ventral part, layer 2/3. Data represent fold expression difference in structure versus grey matter average expression.
Genes with particular expression in the Medial amygdalar nucleus, posterodorsal part, sublayer a. Data represent fold expression difference in structure versus grey matter average expression.
Genes with particular expression in the Retrosplenial area, ventral part, layer 2. Data represent fold expression difference in structure versus grey matter average expression.
Genes with particular expression in the Retrosplenial area, ventral part, layer 5. Data represent fold expression difference in structure versus grey matter average expression.
Genes with particular expression in the Prelimbic area, layer 1. Data represent fold expression difference in structure versus grey matter average expression.
Cerebellum Gene Expression Correlates for ROTASALINE_TIME measured in BXD RI Females & Males obtained using SJUT Cerebellum mRNA M430 (Mar05) RMA. The ROTASALINE_TIME measures Mean time on rotarod following saline under the domain Ethanol. The correlates were thresholded at a p-value of less than 0.001.
Authors:
Philip VM, Duvvuru S, Gomero B, Ansah TA, Blaha CD, Cook MN, Hamre KM, Lariviere WR, Matthews DB, Mittleman G, Goldowitz D, Chesler EJ
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