GeneSet Title
Tier IV
Mouse
1833 Genes
GS411057
LABEL: Sig. YC genes B6 VS yoker vs. yoked-M and yoked-S FDR <= 0.1_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.1.
Tier IV
Mouse
1380 Genes
GS411058
LABEL: Sig. YC genes B6 VS yoker vs. yoked-M and yoked-S FDR <= 0.1_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.1.
Tier IV
Mouse
1381 Genes
GS411059
LABEL: Sig. YC genes B6 VS yoked-M vs. yoker and yoked-S FDR <= 0.1_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.1.
Tier IV
Mouse
1382 Genes
GS411060
LABEL: Sig. YC genes B6 VS yoked-S vs. yoker and yoked-M FDR <= 0.1_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.1.
Tier IV
Mouse
1001 Genes
GS411061
LABEL: Sig. YC genes B6 VS yoker vs. yoked-M and yoked-S FDR <= 0.05_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.05.
Tier IV
Mouse
1002 Genes
GS411062
LABEL: Sig. YC genes B6 VS yoked-M vs. yoker and yoked-S FDR <= 0.05_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.05.
Tier IV
Mouse
1003 Genes
GS411063
LABEL: Sig. YC genes B6 VS yoked-S vs. yoker and yoked-M FDR <= 0.05_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.05.
Tier IV
Mouse
680 Genes
GS411064
LABEL: Sig. YC genes D2 VS yoker vs. yoked-M and yoked-S FDR <= 0.1_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.5-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) genes in D2 mice. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.1.
Tier IV
Mouse
681 Genes
GS411065
LABEL: Sig. YC genes D2 VS yoked-M vs. yoker and yoked-S FDR <= 0.1_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.5-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) genes in D2 mice. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.1.
Tier IV
Mouse
682 Genes
GS411066
LABEL: Sig. YC genes D2 VS yoked-S vs. yoker and yoked-M FDR <= 0.1_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.5-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) genes in D2 mice. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.1.
Tier IV
Mouse
469 Genes
GS411067
LABEL: Sig. YC genes D2 VS yoker vs. yoked-M and yoked-S FDR <= 0.05_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.5-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) genes in D2 mice. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.05.
Tier IV
Mouse
469 Genes
GS411068
LABEL: Sig. YC genes D2 VS yoked-M vs. yoker and yoked-S FDR <= 0.05_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.5-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) genes in D2 mice. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.05.
Tier IV
Mouse
470 Genes
GS411069
LABEL: Sig. YC genes D2 VS yoked-S vs. yoker and yoked-M FDR <= 0.05_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.5-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) genes in D2 mice. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.05.
Tier IV
Mouse
756 Genes
GS411070
LABEL: Sig. YC genes B6 VMB yoker vs. yoked-M and yoked-S FDR <= 0.1_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.1.
Tier IV
Mouse
757 Genes
GS411071
LABEL: Sig. YC genes B6 VMB yoked-M vs. yoker and yoked-S FDR <= 0.1_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.1.
Tier IV
Mouse
757 Genes
GS411072
LABEL: Sig. YC genes B6 VMB yoked-S vs. yoker and yoked-M FDR <= 0.1_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.1.
Tier IV
Mouse
601 Genes
GS411073
LABEL: Sig. YC genes B6 VMB yoker vs. yoked-M and yoked-S FDR <= 0.05_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.05.
Tier IV
Mouse
602 Genes
GS411076
LABEL: Sig. YC genes B6 VMB yoked-M vs. yoker and yoked-S FDR <= 0.05_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.05.
Tier IV
Mouse
603 Genes
GS411077
LABEL: Sig. YC genes B6 VMB yoked-S vs. yoker and yoked-M FDR <= 0.05_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.05.
Tier IV
Mouse
422 Genes
GS411078
LABEL: Sig. YC genes B6 VMB yoker vs. yoked-M and yoked-S FDR <= 0.01_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.01.
Tier IV
Mouse
423 Genes
GS411079
LABEL: Sig. YC genes B6 VMB yoked-M vs. yoker and yoked-S FDR <= 0.01_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.01.
Tier IV
Mouse
424 Genes
GS411080
LABEL: Sig. YC genes B6 VMB yoked-S vs. yoker and yoked-M FDR <= 0.01_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.01.
Tier IV
Mouse
516 Genes
GS411081
LABEL: Sig. YC genes D2 VMB yoker vs. yoked-M and yoked-S FDR <= 0.1_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.5-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) genes D2 mice. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.10.
Tier IV
Mouse
517 Genes
GS411082
LABEL: Sig. YC genes D2 VMB yoked-M vs. yoker and yoked-S FDR <= 0.1_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.5-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) genes D2 mice. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.10.
Tier IV
Mouse
518 Genes
GS411083
LABEL: Sig. YC genes D2 VMB yoked-S vs. yoker and yoked-M FDR <= 0.1_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.5-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) genes D2 mice. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.10.
Tier IV
Mouse
397 Genes
GS411084
LABEL: Sig. YC genes D2 VMB yoker vs. yoked-M and yoked-S FDR <= 0.05_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.5-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) genes in D2 mice. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.05.
Tier IV
Mouse
398 Genes
GS411085
LABEL: Sig. YC genes D2 VMB yoked-M vs. yoker and yoked-S FDR <= 0.05_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.5-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) genes in D2 mice. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.05.
Tier IV
Mouse
399 Genes
GS411086
LABEL: Sig. YC genes D2 VMB yoked-S vs. yoker and yoked-M FDR <= 0.05_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.5-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) genes in D2 mice. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.05.
Tier IV
Mouse
247 Genes
GS411087
LABEL: Sig. YC genes D2 VMB yoker vs. yoked-M and yoked-S FDR <= 0.01_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.5-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) genes in D2 mice. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.01.
Tier IV
Mouse
248 Genes
GS411088
LABEL: Sig. YC genes D2 VMB yoked-M vs. yoker and yoked-S FDR <= 0.01_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.5-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) genes in D2 mice. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.01.
Tier IV
Mouse
249 Genes
GS411089
LABEL: Sig. YC genes D2 VMB yoked-S vs. yoker and yoked-M FDR <= 0.01_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.5-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) genes in D2 mice. Please note that there appears to be an inconsistency in the article for the fold-change threshold between the B6 and D2 animals. From supplementary table 1, FDR <= 0.01.
Tier IV
Mouse
239 Genes
GS411090
LABEL: Sig. B6 VS gene expression correlated with morphine SA_corrDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. FOM and k-means clustering was employed to identify the dominant expression profiles (across yoked conditions) in the YC gene set for the VS and VMB in B6 triads (refer to Supplemental Figure 3, panels A-H). This process identified eight main profiles (clusters A-H), and it should be noted that clusters beyond eight did not substantially improve the adjusted FOM score (Yeung et al., 2001). Four of the clusters (A, C-E) were eliminated from further consideration as a post-hoc t-test with 10% FDR indicated that the majority of genes in the Yokers were not significantly different from either the Yoked-morphine or Yoked-saline animals. These expression patterns would not be indicative of the endpoint we are specifically interested in -contingent self-administration behavior. Of the remaining clusters (B, F-H), B and H were remarkable as they contained genes that appeared to be negatively and positively correlated respectively with the behavioral profile of the B6 triad (compare Supplemental Figure 3B and H to Figure 3). In order to further refine our analysis, we subsequently used the B6 behavioral profile as a template to identify genes in clusters B and H having a significant match to the template (template match p-value < 0.05) (Figure 5), shown here from VS. From supplementary table 2.
Tier IV
Mouse
240 Genes
GS411091
LABEL: Sig. B6 VS gene expression correlated with morphine SA_pvalueDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. FOM and k-means clustering was employed to identify the dominant expression profiles (across yoked conditions) in the YC gene set for the VS and VMB in B6 triads (refer to Supplemental Figure 3, panels A-H). This process identified eight main profiles (clusters A-H), and it should be noted that clusters beyond eight did not substantially improve the adjusted FOM score (Yeung et al., 2001). Four of the clusters (A, C-E) were eliminated from further consideration as a post-hoc t-test with 10% FDR indicated that the majority of genes in the Yokers were not significantly different from either the Yoked-morphine or Yoked-saline animals. These expression patterns would not be indicative of the endpoint we are specifically interested in -contingent self-administration behavior. Of the remaining clusters (B, F-H), B and H were remarkable as they contained genes that appeared to be negatively and positively correlated respectively with the behavioral profile of the B6 triad (compare Supplemental Figure 3B and H to Figure 3). In order to further refine our analysis, we subsequently used the B6 behavioral profile as a template to identify genes in clusters B and H having a significant match to the template (template match p-value < 0.05) (Figure 5), shown here from VS. From supplementary table 2.
Tier IV
Mouse
241 Genes
GS411092
LABEL: Sig. B6 VS gene expression correlated with morphine SA: yoker vs yoked-M and yoked-S_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. FOM and k-means clustering was employed to identify the dominant expression profiles (across yoked conditions) in the YC gene set for the VS and VMB in B6 triads (refer to Supplemental Figure 3, panels A-H). This process identified eight main profiles (clusters A-H), and it should be noted that clusters beyond eight did not substantially improve the adjusted FOM score (Yeung et al., 2001). Four of the clusters (A, C-E) were eliminated from further consideration as a post-hoc t-test with 10% FDR indicated that the majority of genes in the Yokers were not significantly different from either the Yoked-morphine or Yoked-saline animals. These expression patterns would not be indicative of the endpoint we are specifically interested in -contingent self-administration behavior. Of the remaining clusters (B, F-H), B and H were remarkable as they contained genes that appeared to be negatively and positively correlated respectively with the behavioral profile of the B6 triad (compare Supplemental Figure 3B and H to Figure 3). In order to further refine our analysis, we subsequently used the B6 behavioral profile as a template to identify genes in clusters B and H having a significant match to the template (template match p-value < 0.05) (Figure 5), shown here from VS. From supplementary table 2.
Tier IV
Mouse
241 Genes
GS411093
LABEL: Sig. B6 VS gene expression correlated with morphine SA: yoked-M vs yoker and yoked-S_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. FOM and k-means clustering was employed to identify the dominant expression profiles (across yoked conditions) in the YC gene set for the VS and VMB in B6 triads (refer to Supplemental Figure 3, panels A-H). This process identified eight main profiles (clusters A-H), and it should be noted that clusters beyond eight did not substantially improve the adjusted FOM score (Yeung et al., 2001). Four of the clusters (A, C-E) were eliminated from further consideration as a post-hoc t-test with 10% FDR indicated that the majority of genes in the Yokers were not significantly different from either the Yoked-morphine or Yoked-saline animals. These expression patterns would not be indicative of the endpoint we are specifically interested in -contingent self-administration behavior. Of the remaining clusters (B, F-H), B and H were remarkable as they contained genes that appeared to be negatively and positively correlated respectively with the behavioral profile of the B6 triad (compare Supplemental Figure 3B and H to Figure 3). In order to further refine our analysis, we subsequently used the B6 behavioral profile as a template to identify genes in clusters B and H having a significant match to the template (template match p-value < 0.05) (Figure 5), shown here from VS. From supplementary table 2.
Tier IV
Mouse
242 Genes
GS411094
LABEL: Sig. B6 VS gene expression correlated with morphine SA: yoked-S vs yoker and yoked-M_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. FOM and k-means clustering was employed to identify the dominant expression profiles (across yoked conditions) in the YC gene set for the VS and VMB in B6 triads (refer to Supplemental Figure 3, panels A-H). This process identified eight main profiles (clusters A-H), and it should be noted that clusters beyond eight did not substantially improve the adjusted FOM score (Yeung et al., 2001). Four of the clusters (A, C-E) were eliminated from further consideration as a post-hoc t-test with 10% FDR indicated that the majority of genes in the Yokers were not significantly different from either the Yoked-morphine or Yoked-saline animals. These expression patterns would not be indicative of the endpoint we are specifically interested in -contingent self-administration behavior. Of the remaining clusters (B, F-H), B and H were remarkable as they contained genes that appeared to be negatively and positively correlated respectively with the behavioral profile of the B6 triad (compare Supplemental Figure 3B and H to Figure 3). In order to further refine our analysis, we subsequently used the B6 behavioral profile as a template to identify genes in clusters B and H having a significant match to the template (template match p-value < 0.05) (Figure 5), shown here from VS. From supplementary table 2.
Tier IV
Mouse
139 Genes
GS411095
LABEL: Sig. B6 VMB gene expression correlated with morphine SA_corrDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. FOM and k-means clustering was employed to identify the dominant expression profiles (across yoked conditions) in the YC gene set for the VS and VMB in B6 triads (refer to Supplemental Figure 3, panels A-H). This process identified eight main profiles (clusters A-H), and it should be noted that clusters beyond eight did not substantially improve the adjusted FOM score (Yeung et al., 2001). Four of the clusters (A, C-E) were eliminated from further consideration as a post-hoc t-test with 10% FDR indicated that the majority of genes in the Yokers were not significantly different from either the Yoked-morphine or Yoked-saline animals. These expression patterns would not be indicative of the endpoint we are specifically interested in -contingent self-administration behavior. Of the remaining clusters (B, F-H), B and H were remarkable as they contained genes that appeared to be negatively and positively correlated respectively with the behavioral profile of the B6 triad (compare Supplemental Figure 3B and H to Figure 3). In order to further refine our analysis, we subsequently used the B6 behavioral profile as a template to identify genes in clusters B and H having a significant match to the template (template match p-value < 0.05) (Figure 5), shown here from VMB. From supplementary table 2.
Tier IV
Mouse
139 Genes
GS411096
LABEL: Sig. B6 VMB gene expression correlated with morphine SA_pvalueDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. FOM and k-means clustering was employed to identify the dominant expression profiles (across yoked conditions) in the YC gene set for the VS and VMB in B6 triads (refer to Supplemental Figure 3, panels A-H). This process identified eight main profiles (clusters A-H), and it should be noted that clusters beyond eight did not substantially improve the adjusted FOM score (Yeung et al., 2001). Four of the clusters (A, C-E) were eliminated from further consideration as a post-hoc t-test with 10% FDR indicated that the majority of genes in the Yokers were not significantly different from either the Yoked-morphine or Yoked-saline animals. These expression patterns would not be indicative of the endpoint we are specifically interested in -contingent self-administration behavior. Of the remaining clusters (B, F-H), B and H were remarkable as they contained genes that appeared to be negatively and positively correlated respectively with the behavioral profile of the B6 triad (compare Supplemental Figure 3B and H to Figure 3). In order to further refine our analysis, we subsequently used the B6 behavioral profile as a template to identify genes in clusters B and H having a significant match to the template (template match p-value < 0.05) (Figure 5), shown here from VMB. From supplementary table 2.
Tier IV
Mouse
139 Genes
GS411097
LABEL: Sig. B6 VMB gene expression correlated with morphine SA: yoker vs yoked-M and yoked-S_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. FOM and k-means clustering was employed to identify the dominant expression profiles (across yoked conditions) in the YC gene set for the VS and VMB in B6 triads (refer to Supplemental Figure 3, panels A-H). This process identified eight main profiles (clusters A-H), and it should be noted that clusters beyond eight did not substantially improve the adjusted FOM score (Yeung et al., 2001). Four of the clusters (A, C-E) were eliminated from further consideration as a post-hoc t-test with 10% FDR indicated that the majority of genes in the Yokers were not significantly different from either the Yoked-morphine or Yoked-saline animals. These expression patterns would not be indicative of the endpoint we are specifically interested in -contingent self-administration behavior. Of the remaining clusters (B, F-H), B and H were remarkable as they contained genes that appeared to be negatively and positively correlated respectively with the behavioral profile of the B6 triad (compare Supplemental Figure 3B and H to Figure 3). In order to further refine our analysis, we subsequently used the B6 behavioral profile as a template to identify genes in clusters B and H having a significant match to the template (template match p-value < 0.05) (Figure 5), shown here from VMB. From supplementary table 2.
Tier IV
Mouse
140 Genes
GS411098
LABEL: Sig. B6 VMB gene expression correlated with morphine SA: yoked-M vs yoker and yoked-S_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. FOM and k-means clustering was employed to identify the dominant expression profiles (across yoked conditions) in the YC gene set for the VS and VMB in B6 triads (refer to Supplemental Figure 3, panels A-H). This process identified eight main profiles (clusters A-H), and it should be noted that clusters beyond eight did not substantially improve the adjusted FOM score (Yeung et al., 2001). Four of the clusters (A, C-E) were eliminated from further consideration as a post-hoc t-test with 10% FDR indicated that the majority of genes in the Yokers were not significantly different from either the Yoked-morphine or Yoked-saline animals. These expression patterns would not be indicative of the endpoint we are specifically interested in -contingent self-administration behavior. Of the remaining clusters (B, F-H), B and H were remarkable as they contained genes that appeared to be negatively and positively correlated respectively with the behavioral profile of the B6 triad (compare Supplemental Figure 3B and H to Figure 3). In order to further refine our analysis, we subsequently used the B6 behavioral profile as a template to identify genes in clusters B and H having a significant match to the template (template match p-value < 0.05) (Figure 5), shown here from VMB. From supplementary table 2.
Tier IV
Mouse
141 Genes
GS411099
LABEL: Sig. B6 VMB gene expression correlated with morphine SA: yoked-S vs yoker and yoked-M_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. FOM and k-means clustering was employed to identify the dominant expression profiles (across yoked conditions) in the YC gene set for the VS and VMB in B6 triads (refer to Supplemental Figure 3, panels A-H). This process identified eight main profiles (clusters A-H), and it should be noted that clusters beyond eight did not substantially improve the adjusted FOM score (Yeung et al., 2001). Four of the clusters (A, C-E) were eliminated from further consideration as a post-hoc t-test with 10% FDR indicated that the majority of genes in the Yokers were not significantly different from either the Yoked-morphine or Yoked-saline animals. These expression patterns would not be indicative of the endpoint we are specifically interested in -contingent self-administration behavior. Of the remaining clusters (B, F-H), B and H were remarkable as they contained genes that appeared to be negatively and positively correlated respectively with the behavioral profile of the B6 triad (compare Supplemental Figure 3B and H to Figure 3). In order to further refine our analysis, we subsequently used the B6 behavioral profile as a template to identify genes in clusters B and H having a significant match to the template (template match p-value < 0.05) (Figure 5), shown here from VMB. From supplementary table 2.
Tier IV
Mouse
1039 Genes
GS411100
LABEL: DEG B6 VS morphine yoker vs. yoked-S (FDR <= 0.1)_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. FOM and k-means clustering was employed to identify the dominant expression profiles (across yoked conditions) in the YC gene set for the VS and VMB in B6 triads (refer to Supplemental Figure 3, panels A-H). This process identified eight main profiles (clusters A-H), and it should be noted that clusters beyond eight did not substantially improve the adjusted FOM score (Yeung et al., 2001). Four of the clusters (A, C-E) were eliminated from further consideration as a post-hoc t-test with 10% FDR indicated that the majority of genes in the Yokers were not significantly different from either the Yoked-morphine or Yoked-saline animals. These expression patterns would not be indicative of the endpoint we are specifically interested in -contingent self-administration behavior. Of the remaining clusters (B, F-H), B and H were remarkable as they contained genes that appeared to be negatively and positively correlated respectively with the behavioral profile of the B6 triad (compare Supplemental Figure 3B and H to Figure 3). T-tests (at 10%, 5%, and 1% FDR) were conducted to provide additional insight into the genomic response to self- and experimenter- administered drug in VS of B6 mice (shown here) (i.e. Yoker vs yoked-saline and yoked-morphine vs yoked-saline). From supplementary table 3.
Tier IV
Mouse
1040 Genes
GS411101
LABEL: DEG B6 VS yoked-M vs. yoked-S (FDR <= 0.1)_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. FOM and k-means clustering was employed to identify the dominant expression profiles (across yoked conditions) in the YC gene set for the VS and VMB in B6 triads (refer to Supplemental Figure 3, panels A-H). This process identified eight main profiles (clusters A-H), and it should be noted that clusters beyond eight did not substantially improve the adjusted FOM score (Yeung et al., 2001). Four of the clusters (A, C-E) were eliminated from further consideration as a post-hoc t-test with 10% FDR indicated that the majority of genes in the Yokers were not significantly different from either the Yoked-morphine or Yoked-saline animals. These expression patterns would not be indicative of the endpoint we are specifically interested in -contingent self-administration behavior. Of the remaining clusters (B, F-H), B and H were remarkable as they contained genes that appeared to be negatively and positively correlated respectively with the behavioral profile of the B6 triad (compare Supplemental Figure 3B and H to Figure 3). T-tests (at 10%, 5%, and 1% FDR) were conducted to provide additional insight into the genomic response to self- and experimenter- administered drug in VS of B6 mice (shown here) (i.e. Yoker vs yoked-saline and yoked-morphine vs yoked-saline). From supplementary table 3.
Tier IV
Mouse
814 Genes
GS411102
LABEL: DEG B6 VS morphine yoker vs. yoked-S (FDR <= 0.05)_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. FOM and k-means clustering was employed to identify the dominant expression profiles (across yoked conditions) in the YC gene set for the VS and VMB in B6 triads (refer to Supplemental Figure 3, panels A-H). This process identified eight main profiles (clusters A-H), and it should be noted that clusters beyond eight did not substantially improve the adjusted FOM score (Yeung et al., 2001). Four of the clusters (A, C-E) were eliminated from further consideration as a post-hoc t-test with 10% FDR indicated that the majority of genes in the Yokers were not significantly different from either the Yoked-morphine or Yoked-saline animals. These expression patterns would not be indicative of the endpoint we are specifically interested in -contingent self-administration behavior. Of the remaining clusters (B, F-H), B and H were remarkable as they contained genes that appeared to be negatively and positively correlated respectively with the behavioral profile of the B6 triad (compare Supplemental Figure 3B and H to Figure 3). T-tests (at 10%, 5%, and 1% FDR) were conducted to provide additional insight into the genomic response to self- and experimenter- administered drug in VS of B6 mice (shown here) (i.e. Yoker vs yoked-saline and yoked-morphine vs yoked-saline). From supplementary table 3.
Tier IV
Mouse
786 Genes
GS411103
LABEL: DEG B6 VS yoked-M vs. yoked-S (FDR <= 0.05)_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. FOM and k-means clustering was employed to identify the dominant expression profiles (across yoked conditions) in the YC gene set for the VS and VMB in B6 triads (refer to Supplemental Figure 3, panels A-H). This process identified eight main profiles (clusters A-H), and it should be noted that clusters beyond eight did not substantially improve the adjusted FOM score (Yeung et al., 2001). Four of the clusters (A, C-E) were eliminated from further consideration as a post-hoc t-test with 10% FDR indicated that the majority of genes in the Yokers were not significantly different from either the Yoked-morphine or Yoked-saline animals. These expression patterns would not be indicative of the endpoint we are specifically interested in -contingent self-administration behavior. Of the remaining clusters (B, F-H), B and H were remarkable as they contained genes that appeared to be negatively and positively correlated respectively with the behavioral profile of the B6 triad (compare Supplemental Figure 3B and H to Figure 3). T-tests (at 10%, 5%, and 1% FDR) were conducted to provide additional insight into the genomic response to self- and experimenter- administered drug in VS of B6 mice (shown here) (i.e. Yoker vs yoked-saline and yoked-morphine vs yoked-saline). From supplementary table 3.
Tier IV
Mouse
414 Genes
GS411104
LABEL: DEG B6 VS morphine yoker vs. yoked-S (FDR <= 0.01)_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. FOM and k-means clustering was employed to identify the dominant expression profiles (across yoked conditions) in the YC gene set for the VS and VMB in B6 triads (refer to Supplemental Figure 3, panels A-H). This process identified eight main profiles (clusters A-H), and it should be noted that clusters beyond eight did not substantially improve the adjusted FOM score (Yeung et al., 2001). Four of the clusters (A, C-E) were eliminated from further consideration as a post-hoc t-test with 10% FDR indicated that the majority of genes in the Yokers were not significantly different from either the Yoked-morphine or Yoked-saline animals. These expression patterns would not be indicative of the endpoint we are specifically interested in -contingent self-administration behavior. Of the remaining clusters (B, F-H), B and H were remarkable as they contained genes that appeared to be negatively and positively correlated respectively with the behavioral profile of the B6 triad (compare Supplemental Figure 3B and H to Figure 3). T-tests (at 10%, 5%, and 1% FDR) were conducted to provide additional insight into the genomic response to self- and experimenter- administered drug in VS of B6 mice (shown here) (i.e. Yoker vs yoked-saline and yoked-morphine vs yoked-saline). From supplementary table 3.
Tier IV
Mouse
415 Genes
GS411105
LABEL: DEG B6 VS yoked-M vs. yoked-S (FDR <= 0.01)_log2FCDESCRIPTION: Adult male C57BL/6J (B6) and DBA/2J (D2) mice (Jackson Laboratories, Bar Harbor, ME) 60–120 days old and weighing approximately 21–28 g at the start of the experiment were used. D2 (N = 12) and B6 (N = 27) mice were randomly distributed into three different yoked conditions. The self-administration experiment was run using a Yoked-control paradigm with three experimental groups (Yoker, Yoked-morphine, Yoked-saline). After recovery from surgery, the Yokers (subjects with contingent control over morphine injections) were given access to morphine (1mg/kg/injection) on a Fixed Ratio 4 (FR4) schedule of reinforcement in which they had to press the lever 4 times to receive one injection of morphine. Within each genotype, two yoked control animals were paired with the Yoker; one received an injection of 1.0 mg/kg morphine (Yoked-morphine) and the other received an injection of saline (Yoked-saline) each time the Yoker mouse self-injected morphine. All the stimulus conditions surrounding the injection were exactly the same for each member of the trio. Self-administration sessions ran for five days. Animals were housed in the operant chamber with free access to food and water. Each mouse was sacrificed by CO2 asphyxiation and decapitation. Immediately after decapitation the brain was removed and the tissue areas, Ventral Striatum (VS) and Ventral Midbrain (VMB), were dissected and placed in separate tubes with RNA later (Sigma-Aldrich, St. Louis, MO, USA). One group of B6 and D2 animals (N=12 per genotype, thus n=4 complete triads for each genotype) was utilized for behavioral phenotyping, gene expression profiling (microarray) and quantitative real time RT-PCR (qRT-PCR) validation of expression profiles. A second cohort of B6 animals (n = 5 complete triads) served as an independent group to further validate our behavioral results and microarray gene expression findings. The statistical comparisons between genotypes and graphical representations of the data were conducted on the first cohort, since these data represent a direct statistical association between global mRNA expression and behavior. The expression profiles of ~21,000 unique genes were measured in each of the two brain regions (VS and VMB) across the Yoked triad of the B6 and D2 genotypes. Genes exhibiting significant (10% FDR) condition-dependent differences in expression of ~1.4-fold or greater (between any 2 groups) were identified and referred to as significant yoked-condition (YC) B6 genes. FOM and k-means clustering was employed to identify the dominant expression profiles (across yoked conditions) in the YC gene set for the VS and VMB in B6 triads (refer to Supplemental Figure 3, panels A-H). This process identified eight main profiles (clusters A-H), and it should be noted that clusters beyond eight did not substantially improve the adjusted FOM score (Yeung et al., 2001). Four of the clusters (A, C-E) were eliminated from further consideration as a post-hoc t-test with 10% FDR indicated that the majority of genes in the Yokers were not significantly different from either the Yoked-morphine or Yoked-saline animals. These expression patterns would not be indicative of the endpoint we are specifically interested in -contingent self-administration behavior. Of the remaining clusters (B, F-H), B and H were remarkable as they contained genes that appeared to be negatively and positively correlated respectively with the behavioral profile of the B6 triad (compare Supplemental Figure 3B and H to Figure 3). T-tests (at 10%, 5%, and 1% FDR) were conducted to provide additional insight into the genomic response to self- and experimenter- administered drug in VS of B6 mice (shown here) (i.e. Yoker vs yoked-saline and yoked-morphine vs yoked-saline). From supplementary table 3.
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