GeneSet Information

Tier I GS86933 • Table 6: QRT-PCR confirmation of microarray gene expression changes Smoking x schizophrenia interaction: Immune response [DRG]

DESCRIPTION:

Chronic nicotine - Nicotine vs. Control Quantitative reverse transcriptase-polymerase chain reaction Change in gene expression Postmortem parameters, such as age, sex, ethnicity, medication history, mental illness status, cigarette smoking history, and alcohol use, were evaluated through hospital, autopsy, and neuropathology reports. Family members and physicians were also interviewed to detail the smoking and alcohol history of the subject, including packs of cigarettes smoked per day, and the quantity and type of alcohol consumed. All of the subjects who had used alcohol prior to death had consumed no more than two dr two-way ANOVA; Fisher's exact test; Student's t test Values for each sample were normalized to GAPDH. Relative expression values were log10 transformed and differences in mean gene expression were determined using a Student's t test (P < 0.05). For QRT-PCR results, mean relative mRNA amounts +/- standard error of the mean (SEM) for each experimental group were graphed in Sigma Plot 2000. (NIF Table ID 215 [128])

LABEL:

Chronic nicotine - Nicotine vs. Control

SCORE TYPE:

Correlation

DATE ADDED:

2010-11-03

DATE UPDATED:

2024-04-25

SPECIES:

AUTHORS:

Mexal S, Frank M, Berger R, Adams CE, Ross RG, Freedman R, Leonard S

TITLE:

Differential modulation of gene expression in the NMDA postsynaptic density of schizophrenic and control smokers.

JOURNAL:

Brain research. Molecular brain research Oct 2005, Vol 139, pp. 317-32

ABSTRACT:

Nicotine is known to induce the release of multiple neurotransmitters, including glutamate and dopamine, through activation of nicotinic receptors. Gene expression in the N-methyl-d-aspartate postsynaptic density (NMDA-PSD), as well as other functional groups, was compared in postmortem hippocampus of schizophrenic and nonmentally ill smokers and nonsmokers utilizing a microarray and quantitative RT-PCR approach. The expression of 277 genes was significantly changed between all smokers and nonsmokers. Specific gene groups, most notably genes expressed in the NMDA-PSD, were prevalent among these transcripts. Analysis of the interaction between smoking and schizophrenia identified several genes in the NMDA-PSD that were differentially affected by smoking in patients. The present findings suggest that smoking may differentially modulate glutamatergic function in schizophrenic patients and control subjects. The biological mechanisms underlying chronic tobacco use are likely to differ substantially between these two groups. PUBMED: 16122832
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Receptors, Nicotinic (D011978)
Models, Biological (D008954)
alpha Catenin (D051178)
Physicians (D010820)
Patients (D010361)
Postmortem Changes (D011180)
Research Report (D058028)
Blotting, Northern (D015152)
Smoking (D012907)
Hospitals (D006761)
Post-Synaptic Density (D057907)
Microarray Analysis (D046228)
RNA, Messenger (D012333)
Middle Aged (D008875)
Dopamine (D004298)
Receptors, N-Methyl-D-Aspartate (D016194)
History (D006664)
Reverse Transcriptase Polymerase Chain Reaction (D020133)
N-Methylaspartate (D016202)
Tobacco (D014026)
Autopsy (D001344)
Neurotransmitter Agents (D018377)
Schizophrenia (D012559)
Glutamic Acid (D018698)
Blotting, Western (D015153)
Diagnosis-Related Groups (D003953)
Nicotine (D009538)
Analysis of Variance (D000704)
Hippocampus (D006624)
hippocampus (MA:0000191)
immune response (GO:0006955)
postsynaptic density (GO:0014069)
gene expression (GO:0010467)

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