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Tier I GS86623 • Table 1: List of genes differentially expressed in the striatum of A-FOS expressing mice 24 hours after cocaine. [DRG]

DESCRIPTION:

Acute cocaine - A-FOS mouse vs. Littermate control DNA microarray Change in gene expression Animals received a single injection of cocaine (30 mg / kg intraperitoneal) and the striatum harvested at 24 hours. Avadis Microarray analysis software; standard unpaired T-test. A p < 0.001 was used as a cutoff for the selection of genes. Genes are considered up or down regulated if their expression changed 1.4 fold relative to basal conditions. (NIF Table ID 334 [184])

LABEL:

Acute cocaine - A-FOS mouse vs. Littermate control

SCORE TYPE:

Binary

DATE ADDED:

2010-11-03

DATE UPDATED:

2021-07-20

SPECIES:

URI:

http://neuinfo.org/

AUTHORS:

Paletzki RF, Myakishev MV, Polesskaya O, Orosz A, Hyman SE, Vinson C

TITLE:

Inhibiting activator protein-1 activity alters cocaine-induced gene expression and potentiates sensitization.

JOURNAL:

Neuroscience Apr 2008, Vol 152, pp. 1040-53

ABSTRACT:

We have expressed A-FOS, an inhibitor of activator protein-1 (AP-1) DNA binding, in adult mouse striatal neurons. We observed normal behavior including locomotion and exploratory activities. Following a single injection of cocaine, locomotion increased similarly in both the A-FOS expressing and littermate controls. However, following repeated injections of cocaine, the A-FOS expressing mice showed increased locomotion relative to littermate controls, an increase that persisted following a week of withdrawal and subsequent cocaine administration. These results indicate that AP-1 suppresses this behavioral response to cocaine. We analyzed mRNA from the striatum before and 4 and 24 h after a single cocaine injection in both A-FOS and control striata using Affymetrix microarrays (430 2.0 Array) to identify genes mis-regulated by A-FOS that may mediate the increased locomotor sensitization to cocaine. A-FOS expression did not change gene expression in the basal state or 4 h following cocaine treatment relative to controls. However, 24 h after an acute cocaine treatment, 84 genes were identified that were differentially expressed between the A-FOS and control mice. Fifty-six genes are down-regulated while 28 genes are up-regulated including previously identified candidates for addiction including brain-derived neurotrophic factor and period homolog 1. Using a random sample of identified genes, quantitative PCR was used to verify the microarray studies. The chromosomal location of these 84 genes was compared with human genome scans of addiction to identify potential genes in humans that are involved in addiction. PUBMED: 18355967
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Annotation Information

No sequence read archive data associated with this GeneSet.

Gene Expression Regulation (D005786)

Animals (D000818)

Humans (D006801)

Genome, Human (D015894)

DNA (D004247)

Behavior (D001519)

Corpus Striatum (D003342)

Polymerase Chain Reaction (D016133)

Mice (D051379)

Chromosome Mapping (D002874)

Software (D012984)

Behavior, Animal (D001522)

Mice, Transgenic (D008822)

Replication Protein C (D051818)

Adult (D000328)

Microarray Analysis (D046228)

Animals, Newborn (D000831)

RNA, Messenger (D012333)

Therapeutics (D013812)

Neurons (D009474)

Dopamine Uptake Inhibitors (D018765)

Time Factors (D013997)

Cocaine-Related Disorders (D019970)

Oligonucleotide Array Sequence Analysis (D020411)

Cocaine (D003042)

Organization and Administration (D009934)

Brain-Derived Neurotrophic Factor (D019208)

Age Factors (D000367)

Dose-Response Relationship, Drug (D004305)

Locomotion (D008124)

Injections (D007267)

Transcription Factor AP-1 (D018808)

Diagnosis-Related Groups (D003953)

Exploratory Behavior (D005106)

Phosphopyruvate Hydratase (D010751)

striatum (MA:0000891)

addiction (MP:0002555)

DNA binding (GO:0003677)

sensitization (GO:0046960)

locomotion (GO:0040011)

response to cocaine (GO:0042220)

binding (GO:0005488)

behavioral response to cocaine (GO:0048148)

gene expression (GO:0010467)

behavior (GO:0007610)

Trait (VT:0000001)

response to cocaine trait (VT:0010718)

response to addictive substance trait (VT:0010488)

response to xenobiotic stimulus trait (VT:0010487)

organism trait (VT:0010454)

Gene List • 74 Genes

Uploaded As	Gene Symbol Gene Symbol Entrez Ensembl Gene UniGene MGI FlyBase WormBase RGD ZFIN	Homology	Score	Priority	LinkOuts	Emphasis

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Tier I GS86623 • Table 1: List of genes differentially expressed in the striatum of A-FOS expressing mice 24 hours after cocaine. [DRG]

Annotation Information

Gene List • 74 Genes

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Tier I GS86623 • Table 1: List of genes differentially expressed in the striatum of A-FOS expressing mice 24 hours after cocaine. [DRG]

Annotation Information

Gene List • 74 Genes

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