GeneSet Information

Tier III GS75604 • Altered Gene Expression from Patients with Active Celiac Disease

DESCRIPTION:

Analyzed the gene expression profile of a homogeneous population of duodenal epithelial cells taken from patients with active celiac disease, in comparison to control patients. Of the 3,800 genes present on the array (all of which have been previously annotated), 3549 had sufficient data across the five experiments for comparison. Many of these genes showed fold-change ratios with little or no deviation from 1. Thus, to focus on only differentially expressed genes, the list was filtered on a fo

LABEL:

Epithlelial Cel.Dis.

SCORE TYPE:

P-Value

DATE ADDED:

2010-06-22

DATE UPDATED:

2020-05-06

SPECIES:

AUTHORS:

Bracken S, Byrne G, Kelly J, Jackson J, Feighery C

TITLE:

Altered gene expression in highly purified enterocytes from patients with active coeliac disease.

JOURNAL:

BMC genomics Aug 2008, Vol 9, pp. 377

ABSTRACT:

Coeliac disease is a multifactorial inflammatory disorder of the intestine caused by ingestion of gluten in genetically susceptible individuals. Genes within the HLA-DQ locus are considered to contribute some 40% of the genetic influence on this disease. However, information on other disease causing genes is sparse. Since enterocytes are considered to play a central role in coeliac pathology, the aim of this study was to examine gene expression in a highly purified isolate of these cells taken from patients with active disease. Epithelial cells were isolated from duodenal biopsies taken from five coeliac patients with active disease and five non-coeliac control subjects. Contaminating T cells were removed by magnetic sorting. The gene expression profile of the cells was examined using microarray analysis. Validation of significantly altered genes was performed by real-time RT-PCR and immunohistochemistry.Enterocyte suspensions of high purity (98-99%) were isolated from intestinal biopsies. Of the 3,800 genes investigated, 102 genes were found to have significantly altered expression between coeliac disease patients and controls (p < 0.05). Analysis of these altered genes revealed a number of biological processes that are potentially modified in active coeliac disease. These processes include events likely to contribute to coeliac pathology, such as altered cell proliferation, differentiation, survival, structure and transport.This study provides a profile of the molecular changes that occur in the intestinal epithelium of coeliac patients with active disease. Novel candidate genes were revealed which highlight the contribution of the epithelial cell to the pathogenesis of coeliac disease. PUBMED: 18691394
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Annotation Information

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T-Lymphocytes (D013601)
Eating (D004435)
Cell Separation (D002469)
Intestinal Mucosa (D007413)
Magnetics (D008280)
Humans (D006801)
Play and Playthings (D010988)
Cell Proliferation (D049109)
Patients (D010361)
Gene Expression Profiling (D020869)
Biological Processes (D055694)
Case-Control Studies (D016022)
Epithelium (D004848)
Celiac Disease (D002446)
Cornified Envelope Proline-Rich Proteins (D055233)
Adult (D000328)
Pathology (D010336)
Immunohistochemistry (D007150)
Microarray Analysis (D046228)
Infant (D007223)
Middle Aged (D008875)
Proteins (D011506)
Role (D012380)
Connexins (D017630)
Intestines (D007422)
Reverse Transcriptase Polymerase Chain Reaction (D020133)
Enterocytes (D020895)
Oligonucleotide Array Sequence Analysis (D020411)
Glutens (D005983)
Epithelial Cells (D004847)
Cells (D002477)
HLA-DQ Antigens (D006683)
Suspensions (D013535)
Survival (D013534)
Duodenum (D004386)
Biopsy (D001706)
intestine (MA:0000328)
cell (GO:0005623)
cell proliferation (GO:0008283)
pathogenesis (GO:0009405)
gene expression (GO:0010467)
transport (GO:0006810)

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Supported by NIH R01 AA18776 jointly funded by NIAAA and NIDA, and JAX Center for Precision Genetics NIH U54 OD 020351