GeneSet Information

Tier III GS75542 • Genes specifically modified in mouse brain 12 h after R(

DESCRIPTION:

Each mRNA sample group was hybridized to three different arrays, and fold-change values are average of the triplicate measurements; from Parmentier-Batteur et al., 2002

LABEL:

mo R(+)-WIN 55,212-2 12h

SCORE TYPE:

Effect

DATE ADDED:

2010-06-17

DATE UPDATED:

2024-04-25

SPECIES:

AUTHORS:

Parmentier-Batteur S, Jin K, Xie L, Mao XO, Greenberg DA

TITLE:

DNA microarray analysis of cannabinoid signaling in mouse brain in vivo.

JOURNAL:

Molecular pharmacology Oct 2002, Vol 62, pp. 828-35

ABSTRACT:

To identify novel genes involved in cannabinoid receptor-mediated signaling, we used cDNA microarrays to detect changes in mRNA expression in the forebrains of mice 12 h after they were given a single intraperitoneal dose of the naturally-occurring Cannabis sativa alkaloid Delta(9)-tetrahydrocannabinol (Delta(9)-THC) or the synthetic cannabinoid receptor agonist (R)-(+)-2,3-dihydro-5-methyl-3-[(morpholinyl)methyl] pyrrolo[1,2,3-de]-1,4-benzoxazin-yl-1-naphtalenylmethanone mesylate [R(+)-WIN 55,212-2]. Of approximately 11,000 genes from a mouse brain cDNA library that were probed, 65 showed altered (increased or decreased at least 2-fold) expression after exposure to Delta(9)-THC, 41 after exposure to R(+)-WIN 55,212-2, and 20 genes after exposure to both drugs. Genes affected similarly by Delta(9)-THC and R(+)-WIN 55,212-2 were considered likely to reflect cannabinoid receptor activation, and expression of the protein products of two such genes not previously implicated in cannabinoid signaling-melanocyte-specific gene-related gene 1 (MRG1) and hexokinase 4 (glucokinase, GK)-was measured by Western blotting and immunohistochemistry. Western blots showed approximately 2-fold increases in the levels of both proteins in mouse forebrain. Immunohistochemistry revealed preferential localization of MRG1 to cerebral blood vessels and of GK to hypothalamic neurons. These findings suggest that MRG1 and GK are cannabinoid-regulated genes and that they may be involved in the vascular and hypothalamic effects of cannabinoids, respectively. PUBMED: 12237329
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Annotation Information

No sequence read archive data associated with this GeneSet.


Brain (D001921)
Gene Expression Regulation (D005786)
Animals (D000818)
Glucokinase (D005941)
Benzoxazines (D048588)
Libraries (D007990)
Gene Library (D015723)
DNA (D004247)
Tetrahydrocannabinol (D013759)
Mice (D051379)
Gene Expression Profiling (D020869)
Blood Vessels (D001808)
Mesylates (D008698)
Immunohistochemistry (D007150)
Microarray Analysis (D046228)
RNA, Messenger (D012333)
Proteins (D011506)
Prosencephalon (D016548)
Hallucinogens (D006213)
Neurons (D009474)
Trans-Activators (D015534)
Pharmaceutical Preparations (D004364)
Oligonucleotide Array Sequence Analysis (D020411)
Repressor Proteins (D012097)
Blood (D001769)
DNA, Complementary (D018076)
Naphthalenes (D009281)
Morpholines (D009025)
DNA-Binding Proteins (D004268)
Signal Transduction (D015398)
Cannabis (D002188)
Cannabinoids (D002186)
Hexokinase (D006593)
Blotting, Western (D015153)
Receptors, Cannabinoid (D043882)
Analgesics (D000700)
blood (MA:0000059)
forebrain (MA:0000170)
brain (MA:0000168)
localization (GO:0051179)
signaling (GO:0023052)

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Supported by NIH R01 AA18776 jointly funded by NIAAA and NIDA, and JAX Center for Precision Genetics NIH U54 OD 020351