DESCRIPTION:
RNA‐seq was used to investigate the pathology of oxycodone abuse. In order to uncover the potential epitranscriptomic role of m6A methylation in oxycodone abuse, three different doses of saline or oxycodone (1.5, 3.0, and 6.0mg/kg) were administered intraperitoneally (i.p., 0.1mL/10g) to a group of mice for 9 days. Subsequently, after 4 days of oxycodone withdrawal and 1hr after open field testing, striatum samples from the experimental mice were collected for RNA‐sequencing (RNA‐seq) to detect m6A methylation‐associated enzymes and then examine m6A‐related epigenetic alterations. Total RNA was extracted from the striatum (n=3 C57BL/6J mice in each group), and the quantification of lncRNA, ncRNA, and mRNA followed. Quality‐checked libraries were sequenced on the DNBseq platform using MGISEQ‐2000 with 100PE sequencing. Library construction, lncRNA‐seq, ncRNA‐seq, and RNA‐seq, as well as data collection and mapping were outsourced to HuaDa Gene Biotechnology (Shenzhen, China). From supplementary table 1. Please note that the upregulated genes reported in the Results section correspond to negative fold change values in the table, and downregulated correspond to positive fold change values - this may or may not be an error in reporting by the authors.
LABEL:
DEG (ncRNA) mouse striatum oxycodone addiction_qvalue
SCORE TYPE:
Q-Value
THRESHOLD:
<= 0.5
GENES IN THRESHOLD:
29
DATE ADDED:
DATE UPDATED:
SPECIES:
AUTHORS:
TITLE:
JOURNAL:
ABSTRACT:
Genes in threshold: 29
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