GeneSet Information

Tier IV GS409802 • Differentially expressed genes in rat medial preoptic area (MPOA) mu opioid receptor (Oprm1) knockdown vs control_pvalue

DESCRIPTION:

Differential gene expression was evaluated between male Sprague Dawley rats in the Oprm1 knockdown (a mu opioid receptor) and control groups. Effects of Oprm1 downregulation in the medial preoptic area (MPOA) on patterns of gene expression in the MPOA were examined using RNA-Seq. Paired-end 2x150 bp sequencing was performed, using standard SBS chemistry (v3) on an Illumina NovaSeq6000 sequencer. We conducted differential gene expression analysis of RNA-Seq data using the EdgeR Bioconductor Package, v. 3.9. The final samples for RNA-Seq analysis were Scr shRNA control (n=7); Oprm1 shRNA knockdown (n=5). In the behavior experiments, Oprm1 downregulation produced less social play behavior in male rats.

LABEL:

DEG rat MPOA Oprm1 knockdown vs control_pvalue

SCORE TYPE:

P-Value

THRESHOLD:

<= 0.5

GENES IN THRESHOLD:

5081

DATE ADDED:

2024-12-30

DATE UPDATED:

2024-12-30

SPECIES:

AUTHORS:

Changjiu Zhao, Liza Chang, Anthony P Auger, Stephen C Gammie, Lauren V Riters

TITLE:

Mu opioid receptors in the medial preoptic area govern social play behavior in adolescent male rats.

JOURNAL:

Genes, brain, and behavior Sep 2020, Vol 19, pp. e12662

ABSTRACT:

Neural systems underlying important behaviors are usually highly conserved across species. The medial preoptic area (MPOA) has been demonstrated to play a crucial role in reward associated with affiliative, nonsexual, social communication in songbirds. However, the role of MPOA in affiliative, rewarding social behaviors (eg, social play behavior) in mammals remains largely unknown. Here we applied our insights from songbirds to rats to determine whether opioids in the MPOA govern social play behavior in rats. Using an immediate early gene (ie, Egr1, early growth response 1) expression approach, we identified increased numbers of Egr1-labeled cells in the MPOA after social play in adolescent male rats. We also demonstrated that cells expressing mu opioid receptors (MORs, gene name Oprm1) in the MPOA displayed increased Egr1 expression when adolescent rats were engaged in social play using double immunofluorescence labeling of MOR and Egr1. Furthermore, using short hairpin RNA-mediated gene silencing we revealed that knockdown of Oprm1 in the MPOA reduced the number of total play bouts and the frequency of pouncing. Last, RNA sequencing differential gene expression analysis identified genes involved in neuronal signaling with altered expression after Oprm1 knockdown, and identified Egr1 as potentially a key modulator for Oprm1 in the regulation of social play behavior. Altogether, these results show that the MPOA is involved in social play behavior in adolescent male rats and support the hypothesis that the MPOA is part of a conserved neural circuit across vertebrates in which opioids act to govern affiliative, intrinsically rewarded social behaviors. PUBMED: 32388931
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Annotation Information

No sequence read archive data associated with this GeneSet.


Growth (D006128)
Social Control, Formal (D012926)
Mammals (D008322)
RNA (D012313)
Play and Playthings (D010988)
Behavior (D001519)
Gene Expression Profiling (D020869)
Gene Silencing (D020868)
Genes, Immediate-Early (D017781)
Gene Expression (D015870)
Adolescent (D000293)
Chemistry (D002621)
Role (D012380)
Down-Regulation (D015536)
Communication (D003142)
Receptors, Opioid, mu (D017450)
RNA, Small Interfering (D034741)
Rats (D051381)
Genes (D005796)
Cells (D002477)
Sequence Analysis, RNA (D017423)
Preoptic Area (D011301)
Fluorescent Antibody Technique (D005455)
Reward (D012201)
Control Groups (D035061)
Vertebrates (D014714)
Social Behavior (D012919)
Receptors, Opioid (D011957)
Songbirds (D020308)
Analgesics, Opioid (D000701)
Rats, Sprague-Dawley (D017207)
negative regulation of gene expression, epigenetic (GO:0045814)
biological regulation (GO:0065007)
gene silencing by RNA (GO:0031047)
negative regulation of gene expression (GO:0010629)
growth (GO:0040007)
gene expression (GO:0010467)
behavior (GO:0007610)
signaling (GO:0023052)
RNA (EDAM_topic:0099)
Data acquisition and deposition (EDAM_topic:3077)
Gene expression analysis (EDAM_operation:2410)
Analysis (EDAM_operation:2945)
Genetics (EDAM_topic:3053)
Vertebrates (EDAM_topic:2820)
Cell biology resources (EDAM_topic:2229)
Gene name (EDAM_data:2299)
Sequencing (EDAM_topic:3168)
Microarray data (EDAM_data:2603)
Transcriptomics (EDAM_topic:0203)
Data (EDAM_data:0006)
Mammals (EDAM_topic:3048)
Gene expression data processing (EDAM_operation:2495)
RNA-Seq (EDAM_topic:3170)
Differential expression analysis (EDAM_operation:3223)
rna (EDAM_format:1213)
maleate(2-) (CHEBI:30780)
ribonucleic acid (CHEBI:33697)
role (CHEBI:50906)
RNA-seq evidence (ECO:0000295)
immunofluorescence evidence (ECO:0000007)
Brooke-Spiegler syndrome (DOID:0050693)
Sprague Dawley (EFO:0001352)
labelling (EFO:0000562)
gene name (EFO:0002605)
medial (EFO:0001660)
MOR (EFO:0006655)
transcription profiling by high throughput sequencing (EFO:0002770)
area (EFO:0001696)
control (EFO:0001461)
sequencer (EFO:0003739)
immunofluorescence (MMO:0000662)
RNA-seq assay (MMO:0000659)
preoptic area (UBERON:0001928)
rostral octaval nerve motor nucleus (UBERON:2002175)
medial preoptic region (UBERON:0007769)
male organism (UBERON:0003101)

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Supported by NIH R01 AA18776 jointly funded by NIAAA and NIDA, and JAX Center for Precision Genetics NIH U54 OD 020351

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