DESCRIPTION:
Mice exposed to morphine drank about 5mL per animal per day (Fig. 1A). Male and female mice on a C57Bl/6J background were used in all studies. Experiments to manipulate mu-opioid receptor-expressing neurons used MOR-Cre mice developed in our lab. These MOR-Cre mice were then crossed with RosaLSLSun1-sfGFP [29] to generate a reporter line for visualization of MORs or Rosa26LSL-EGFP-L10a [30] for cell-type specific RNA sequencing. Mice were 8–12 weeks at the start of each experiment. Mice were sacrificed between 1200 and 1400h and, for each mouse, a gross dissection targeted at the PVT was used to generate tissue for the translating ribosome affinity purification (TRAP). We used both control mice and mice exposed to chronic morphine for TRAP. Due to the small amount of brain tissue, 2 PVT dissections were pooled together to create each biological sample—males were only pooled with males and females were only pooled with females, for a total of four biological replicates for each of the morphine and control groups. RNA sequencing of the MOR+expressing neurons in the PVT that were affinity purified by Translating Ribosome Affinity Purification (TRAP) and subsequent DE analysis identified 311 upregulated and 211 downregulated genes in the morphine group as compared to the control group (multiple-testing-adjusted P value <0.05).
LABEL:
DEG mouse PVT MOR's morphine vs control_pvalue
SCORE TYPE:
P-Value
THRESHOLD:
<= 0.5
GENES IN THRESHOLD:
520
DATE ADDED:
DATE UPDATED:
SPECIES:
AUTHORS:
TITLE:
JOURNAL:
ABSTRACT:
Genes in threshold: 520
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