DESCRIPTION:
Relationship of cumulative fentanyl intake, opioid receptors and pattern recognition receptors. Male, Sprague-Dawley rats (n = 17); Envigo, Indianapolis, IN) weighing between 250 and 300 g at the start of experiments were used. Rats were trained to lever press for fentanyl (0.0032 mg/kg/0.1 mL infusion) or saline (0.1 mL/inf) using established methods (Neelakantan et al., 2017). Two rats were excluded due to loss of catheter patency. Twenty-four hours following the final self-administration session, rats were sacrificed for molecular biology analyses (n = 15). Due to individual differences and variability in the levels of opioid intake, animals sacrificed for molecular analyses were stratified according to lifetime fentanyl intake: 0 mg/kg fentanyl [i.e., saline self-administration rats (n = 5)], <1.2 mg/kg fentanyl [“low” self-administration rats (n = 5)] and >1.2 mg/kg fentanyl [“high” self-administration rats (n = 5)]. Pearson’s product-moment correlation coefficient was computed to assess the relationship between the levels of cumulative fentanyl intake and opioid receptor (OR) expression, mitochondrial antiviral signaling protein (MAVS) expression or stimulator of interferon genes (STING) expression levels in different brain regions (hippocampus (HIP), nucleus accumbens (NAc), and prefrontal cortex (PFC)), at both RNA and protein levels. The correlation coefficient (r) demonstrates the strength and direction of relationship and significance (p value) is given for each individual correlation (n = 11). The overall level of significance was set at 0.05 and two-tailed p-values reported to assess the relationships.
LABEL:
Sig. relationship between immune RNA expression and lifetime fentanyl intake rat HIP_corr
SCORE TYPE:
Correlation
THRESHOLD:
<= 0.5
GENES IN THRESHOLD:
0
DATE ADDED:
DATE UPDATED:
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AUTHORS:
TITLE:
JOURNAL:
ABSTRACT:
Genes in threshold: 0
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