DESCRIPTION:
Tissue from 46 adult male rhesus macaques (Macaca mulatta), aged 5 to 11 years, was used in this study. These animals, individually housed at the Oregon National Primate Research Center, were induced to drink ethanol by schedule-induced polydipsia per previously published methods (Grant et al., 2008; Helms et al., 2014), and were then allowed 22 h per day of ad libitum access to water and 4% (w/v) ethanol in water for a period of 1 year. Control animals were age-matched within cohorts, were given daily maltose dextran solution (calorically matched to an ethanol drinker) and had access to water during all portions of the experiment. After 1 year of open access to ethanol, necropsy was performed within 4 h of last access to ethanol, and tissue deposited into the Monkey Alcohol Tissue Research Resource (MATRR). No actual animal experiments were conducted during these studies. The animals used for these studies comprised MATRR rhesus cohorts 4, 5, 7a, and 7b. Samples of monkey medial prefrontal cortex (PFC) (anterior cingulate and subgenual cortex; Brodmann areas 24, 25, and 32) were obtained from the MATRR. Affymetrix GeneChip® Rhesus Macaque Genome Arrays were used to measure monkey gene expression. Monkey RNA samples were processed for microarray analysis in two groups (cohorts 4 and 5, followed by cohorts 7a and 7b) consisting of eight batches processed in a supervised randomization scheme to minimize batch effects, as described previously by our laboratories (Kerns et al., 2005; Wolen and Miles, 2012; Smith et al., 2016). Microarrays for two animals failed quality control standards, and one animal was deemed an outlier by hierarchical clustering of RMA data, leaving 43 animals/arrays (32 ethanol drinking, 11 control).
LABEL:
DEG macaque PFC microarray EtOH vs. control_pvalue
SCORE TYPE:
P-Value
DATE ADDED:
DATE UPDATED:
SPECIES:
AUTHORS:
TITLE:
JOURNAL:
ABSTRACT:
Uploaded As | Gene Symbol | Homology | Score | Priority | LinkOuts | Emphasis |
---|