DESCRIPTION:
We used a two-bottle free-choice paradigm to measure alcohol drinking [42], and studied effect of chronic alcohol consumption during adolescent development. Male adolescent ICR (outbred) mice (3 weeks of age) were divided into two alcohol groups, one with free access to 5% alcohol, and the other to 10% alcohol, so as to determine if there was a difference in alcohol consumption behavior due to difference in alcohol concentration. In addition, a water-only control group (each mouse had 2 bottles, both filled with water) was used. For brain tissue processing for gene expression analysis, 19 mice with 7 weeks alcohol exposure were selected and grouped into alcohol sample groups (n=1–3 per sample group). Samples A1-A3 had mice with relatively less alcohol consumed (0.50±0.38 g/kg/day); samples A4-A9 had mice with relatively more alcohol consumed (4.80±0.36 g/kg/day); these were significantly different (p<0.05, Unpaired t test with Welch's correction). The rationale for such an approach of sample pooling was to cover the spectrum of mouse alcohol consumption behavior, so that the underlying gene expression patterns may be discernible. For water-only controls, nine mice were selected randomly, and grouped into water sample groups (n=3 per sample group), designated samples C1-C3. RNA samples extracted from hypothalamus were used in gene expression analysis, using the Agilent mouse whole genome microarray chip set.
LABEL:
DEG microarray ICR mouse hypothalamus chronic alcohol vs. control_pvalue
SCORE TYPE:
P-Value
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