DESCRIPTION:
Male C57BL/6J and DBA/2J mice (10 weeks old upon arrival) were purchased from the Jackson Laboratory and assigned to either the air control or CIE group. Mice were allowed to self-administer alcohol (15% v/v vs. water) for 2 h a day (5 days a week) 6 weeks prior to treatment in order to establish baseline consumption. Access to 15% alcohol versus water started 30 min prior to the start of the dark cycle. Following establishment of baseline drinking, two male mice representative of each strain were separated into two groups to be exposed to either weekly cycles of CIE exposure (CIE group) or air control (AIR group) exposure. There was no difference in baseline consumption or preference within strain prior to assignment to AIR or CIE treatment groups (Supplemental Fig. 1). As expected, there was a significant difference in alcohol consumption and preference between strains at baseline (B6 > D2, p < 0.01. Mice assigned to the CIE treatment group were exposed to alcohol vapor for 16 h a day followed by 8 h of withdrawal for 4 days. Following the fourth vapor exposure, mice were given a 72-h abstinence period before resuming ethanol intake in the home cage for 5 days. Mice in the AIR control treatment group were similarly treated but exposed only to air in the inhalation chambers. This pattern of CIE or air control exposure followed by 5 days of ethanol self-administration was repeated for four cycles. A fifth cycle of CIE (or air) exposure followed the fourth ethanol intake evaluation period, and brain tissue was collected 72 h after the last cycle ended. Poly-A enriched mRNA was sequenced on two platforms, ABI SOLID 550XL Wildfire (65 samples) and Ion Proton (39 samples). Differential gene expression was determined using the glm function in R where gene expression (Y) is dependent on treatment, strain, region, and treatment interactions with strain and region: Y ∼ Treatment + Strain + Region + Treatment*Strain + Treatment*Region. Treatment was coded as 0 for AIR and 1 for CIE, 0 for B6 and 1 for D2. Regions were collapsed into three groups based on PCA clustering and coded as 0 for hippocampus (CA1 and CA3 regions), 1 for cortex (Prl, ILC, and VCX), and 2 for the remaining subcortical and limbic and mesolimbic tissue (VTA, NAc, NAs, DMS, CeA, and BST).
LABEL:
DEG effect of treatment (CIE vs Air) x region interaction C57BL/6J and DBA/2J brains (p < 0.01)_pvalue
SCORE TYPE:
P-Value
THRESHOLD:
<= 0.5
GENES IN THRESHOLD:
60
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DATE UPDATED:
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ABSTRACT:
Genes in threshold: 60
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