GeneSet Information

Tier IV GS408304 • Differentially expressed genes in FACS-purified oligodendrocytes in male C57BL/6 mice 4h after saline or morphine injection_qvalue_0.05subset

DESCRIPTION:

Subset dataset of differentially expressed genes at padj < 0.05 of GS407887.

LABEL:

Morphine_FACS-purified_oligodendrocytes_C57BL/6_qvalue_0.05subset

SCORE TYPE:

Q-Value

THRESHOLD:

<= 0.5

GENES IN THRESHOLD:

4498

DATE ADDED:

2024-03-14

DATE UPDATED:

2024-10-22

SPECIES:

AUTHORS:

Denis Avey, Sumithra Sankararaman, Aldrin K Y Yim, Ruteja Barve, Jeffrey Milbrandt, Robi D Mitra

TITLE:

Single-Cell RNA-Seq Uncovers a Robust Transcriptional Response to Morphine by Glia.

JOURNAL:

Cell reports Sep 2018, Vol 24, pp. 3619-3629.e4

ABSTRACT:

Molecular and behavioral responses to opioids are thought to be primarily mediated by neurons, although there is accumulating evidence that other cell types play a prominent role in drug addiction. To investigate cell-type-specific opioid responses, we performed single-cell RNA sequencing (scRNA-seq) of the nucleus accumbens of mice following acute morphine treatment. Differential expression analysis uncovered unique morphine-dependent transcriptional responses by oligodendrocytes and astrocytes. We examined the expression of selected genes, including Cdkn1a and Sgk1, by FISH, confirming their induction by morphine in oligodendrocytes. Further analysis using RNA-seq of FACS-purified oligodendrocytes revealed a large cohort of morphine-regulated genes. The affected genes are enriched for roles in cellular pathways intimately linked to oligodendrocyte maturation and myelination, including the unfolded protein response. Altogether, our data illuminate the morphine-dependent transcriptional response by oligodendrocytes and offer mechanistic insights into myelination defects associated with opioid abuse. PUBMED: 30257220
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Annotation Information

No sequence read archive data associated with this GeneSet.


Unfolded Protein Response (D056811)
RNA (D012313)
Mice (D051379)
Role (D012380)
Neurons (D009474)
Nucleus Accumbens (D009714)
Genes (D005796)
Cells (D002477)
RNA, Small Cytoplasmic (D020733)
Morphine (D009020)
accumbens nucleus (MA:0000892)
addiction (MP:0002555)
nucleus (GO:0005634)
myelination (GO:0042552)
RNA (EDAM_topic:0099)
Analysis (EDAM_operation:2945)
Genetics (EDAM_topic:3053)
Fish (EDAM_topic:0210)
Sequencing (EDAM_topic:3168)
protein (EDAM_format:1208)
Transcriptomics (EDAM_topic:0203)
Data (EDAM_data:0006)
Gene expression data processing (EDAM_operation:2495)
Evidence (EDAM_data:2042)
Pathways, networks and models (EDAM_topic:0602)
RNA-Seq (EDAM_topic:3170)
Differential expression analysis (EDAM_operation:3223)
rna (EDAM_format:1213)
Type (EDAM_data:2100)
morphine (CHEBI:17303)
protein (CHEBI:36080)
drug (CHEBI:23888)
protein polypeptide chain (CHEBI:16541)
ribonucleic acid (CHEBI:33697)
role (CHEBI:50906)
propyzamide (CHEBI:34935)
atomic nucleus (CHEBI:33252)
morphine(1+) (CHEBI:58097)
Acute (HP:0011009)
RNA-seq evidence (ECO:0000295)
evidence (ECO:0000000)
opioid abuse (DOID:11206)
opioid dependence (EFO:0005611)
treatment (EFO:0000727)
obsolete_accumbens nucleus (EFO:0000906)
transcription profiling by high throughput sequencing (EFO:0002770)
drug dependence (EFO:0003890)
cohort (EFO:0004445)
RNA-seq assay (MMO:0000659)
fluorescence-activated cell sorting method (MMO:0000310)
rostral octaval nerve motor nucleus (UBERON:2002175)
neural nucleus (UBERON:0000125)
nucleus accumbens (UBERON:0001882)

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Genes in threshold: 4498

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Supported by NIH R01 AA18776 jointly funded by NIAAA and NIDA, and JAX Center for Precision Genetics NIH U54 OD 020351

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