DESCRIPTION:

QTL Associated with Blood pressure. On Chromosome 2 with a LOD score= , p-value =5.00E-04. From a(n) congenic of SS/JrxSR/Jr

LABEL:

QTL-Bp132-Rat-Chr 2

SCORE TYPE:

Binary

DATE ADDED:

2015-06-10

DATE UPDATED:

2024-04-25

SPECIES:

AUTHORS:

Jeffs B, Negrin CD, Graham D, Clark JS, Anderson NH, Gauguier D, Dominiczak AF

TITLE:

Applicability of a "speed" congenic strategy to dissect blood pressure quantitative trait loci on rat chromosome 2.

JOURNAL:

Hypertension None None, Vol 35, pp. 179-87

ABSTRACT:

1. Hypertension. 2000 Jan;35(1 Pt 2):179-87. Applicability of a "speed" congenic strategy to dissect blood pressure quantitative trait loci on rat chromosome 2. Jeffs B(1), Negrin CD, Graham D, Clark JS, Anderson NH, Gauguier D, Dominiczak AF. Author information: (1)Department of Medicine and Therapeutics, University of Glasgow, Western Infirmary, Glasgow, UK. anna.dominiczak@clinmed.gla.ac.uk The identification of any quantitative trait locus (QTL) via a genome scan is only the first step toward the ultimate goal of gene identification. The next step is the production of congenic strains by which the existence of a QTL may be verified and the implicated chromosomal region be reduced to a size applicable to positional cloning of the causal gene. We used a speed congenic breeding protocol previously verified in mice for 2 blood pressure QTLs on rat chromosome 2. Four congenic strains were produced through introgression of various segments of chromosome 2 from Wistar-Kyoto rats from Glasgow colonies [WKY((Gla)) rats] into the recipient stroke-prone spontaneously hypertensive rats from Glasgow colonies [SHRSP((Gla))], and vice versa. The number of backcross generations required for each strain to achieve complete homozygosity at 83 background genetic markers in a "best" male varied between 3 and 4. Transfer of the region of rat chromosome 2 containing both QTLs from WKY((Gla)) into an SHRSP((Gla)) genetic background lowered both baseline and salt-loaded systolic blood pressure by approximately 20 and approximately 40 mm Hg in male congenic rats compared with the SHRSP parental strain (F=53.4, P<0.005; F=28.0, P< 0.0005, respectively). In contrast, control animals for stowaway heterozygosity presented no deviation from the blood pressure values recorded for the SHRSP((Gla)), indicating that if such heterozygosity exists, its effect on blood pressure is negligible. A reciprocal strategy in which 1 or both QTLs on rat chromosome 2 were transferred from SHRSP((Gla)) into a WKY((Gla)) genetic background resulted in statistically significant but smaller blood pressure increases for 1 of these QTLs. These results confirm the existence of blood pressure QTLs on rat chromosome 2 and demonstrate the applicability of a speed congenic strategy in the rat and emphasize the important role of the genetic background. PMID: 10642295 [PubMed - indexed for MEDLINE] PUBMED: 10642295
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