1. Hypertension. 2002 Feb;39(2 Pt 2):348-52.
Heart rate and blood pressure quantitative trait loci for the airpuff startle
reaction.
Jaworski RL(1), Jirout M, Closson S, Breen L, Flodman PL, Spence MA, Kren V,
Krenova D, Pravenec M, Printz MP.
Author information:
(1)Department of Pharmacology, University of California at San Diego, 92093-0636,
USA.
The airpuff startle reaction is a probe of sensori-autonomic processing and is
useful for studies of genetic control of stress-induced cardiovascular activity.
Using a Wistar-Kyoto-Spontaneously Hypertensive Rat F2 cross, we reported an
airpuff-elicited strain-dependent and trial-dependent bradycardia, the absence of
which cosegregated with hypertension. Here, we use the mapping power of the
HXB-BXH recombinant inbred rat strains (n=23) to locate quantitative trait loci
(QTL) for this and associated cardiovascular phenotypes. Rats (12 weeks old),
with indwelling femoral arterial catheters, were subjected to repeated airpuff
startle stimuli (100 ms, 12.5 psi, 28 trials). Basal mean arterial pressure
(MAP), delta MAP, and delta heart rate response to airpuff stimuli were analyzed
as the average over 28 trials. There was a significant strain effect on the
cardiovascular phenotypes measured. One QTL for the bradycardia elicited by the
first airpuff stimulus was identified on chromosome 2 (D2rat 62/63; logarithm of
odds [LOD] 2.9) mapping near a reported blood pressure locus. Further QTL were
identified for basal MAP (RN08), stimulus-elicited tachycardia on trials 2 to 5
(RNO1 and RNO10), and delta MAP (RNO6). Our results indicate that chromosomes 1,
2, and 10 are involved in heart rate responses to airpuff startle stimulus, and
chromosomes 6 and 8 are involved in pressor responses. This study is the first to
identify stress-related heart rate loci and provides additional support for our
prior cosegregation results. Furthermore, we have established the utility of this
experimental paradigm to identify loci responsible for cardiovascular regulation
during stress in genetic hypertensive models.
PMID: 11882571 [PubMed - indexed for MEDLINE]
PUBMED: 11882571
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