1. Genome Res. 2009 Jan;19(1):150-8. doi: 10.1101/gr.081497.108. Epub 2008 Oct 29.
A resource for the simultaneous high-resolution mapping of multiple quantitative
trait loci in rats: the NIH heterogeneous stock.
Johannesson M(1), Lopez-Aumatell R, Stridh P, Diez M, Tuncel J, Blázquez G,
Martinez-Membrives E, Cañete T, Vicens-Costa E, Graham D, Copley RR,
Hernandez-Pliego P, Beyeen AD, Ockinger J, Fernández-Santamaría C, Gulko PS,
Brenner M, Tobeña A, Guitart-Masip M, Giménez-Llort L, Dominiczak A, Holmdahl R,
Gauguier D, Olsson T, Mott R, Valdar W, Redei EE, Fernández-Teruel A, Flint J.
Author information:
(1)Wellcome Trust Centre for Human Genetics, Oxford OX3 7BN, United Kingdom.
The laboratory rat (Rattus norvegicus) is a key tool for the study of medicine
and pharmacology for human health. A large database of phenotypes for integrated
fields such as cardiovascular, neuroscience, and exercise physiology exists in
the literature. However, the molecular characterization of the genetic loci that
give rise to variation in these traits has proven to be difficult. Here we show
how one obstacle to progress, the fine-mapping of quantitative trait loci (QTL),
can be overcome by using an outbred population of rats. By use of a genetically
heterogeneous stock of rats, we map a locus contributing to variation in a
fear-related measure (two-way active avoidance in the shuttle box) to a region on
chromosome 5 containing nine genes. By establishing a protocol measuring multiple
phenotypes including immunology, neuroinflammation, and hematology, as well as
cardiovascular, metabolic, and behavioral traits, we establish the rat HS as a
new resource for the fine-mapping of QTLs contributing to variation in complex
traits of biomedical relevance.
PMCID: PMC2612958
PMID: 18971309 [PubMed - indexed for MEDLINE]
PUBMED: 18971309
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