1. Am J Hypertens. 1999 Nov;12(11 Pt 1):1098-104.
Aging and salt-loading modulate blood pressure QTLs in rats.
Mashimo T(1), Nabika T, Matsumoto C, Tamada T, Ueno K, Sawamura M, Ikeda K, Kato
N, Nara Y, Yamori Y.
Author information:
(1)Department of Life Science, Graduate School of Human and Environmental
Studies, Kyoto University, Japan.
To evaluate the effects of nongenetic factors, aging, and salt-loading on the
quantitative trait loci (QTLs) for blood pressure (BP), we conducted a
genome-wide linkage analysis using multiple sets of BP measurements in 125 male
F2 generation cross derived from stroke-prone spontaneously hypertensive rats and
normotensive Wistar-Kyoto rats. The experiment was arranged in two stages. In the
first stage, corresponding to the developing period of the rats, BP was measured
repeatedly without loading of salt; this continued until the rats were 5 months
of age. In the second stage, after the baseline BP leveled off, 1% salt water was
given to the rats and BP was monitored for the subsequent 7 months. Genome
scanning was performed using 201 markers. In the developing period, three QTLs
were identified on chromosomes 1, 3, and 4 (logarithmic odds [LOD] scores of 5.6,
3.1, and 3.2, respectively), which had peaks at 8 or 10 weeks of age. In the
latter salt-loading stage, QTLs for BP were detected on chromosomes 1 and 10 (LOD
scores 4.6 and 4.5, respectively). When the BP increase during salt-loading was
analyzed as a phenotype, however, only the region on chromosome 10 showed linkage
at a suggestive level (LOD score 3.2). The present study provides experimental
evidence that QTLs for BP could be modulated by nongenetic factors, such as aging
and salt-loading.
PMID: 10604486 [PubMed - indexed for MEDLINE]
PUBMED: 10604486
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