1. Genomics. 2007 Mar;89(3):343-53. Epub 2007 Jan 10.
Fine-mapping and comprehensive transcript analysis reveals nonsynonymous variants
within a novel 1.17 Mb blood pressure QTL region on rat chromosome 10.
Saad Y(1), Garrett MR, Manickavasagam E, Yerga-Woolwine S, Farms P, Radecki T,
Joe B.
Author information:
(1)Department of Physiology, Pharmacology, Metabolism, and Cardiovascular
Sciences, University of Toledo, Health Science Campus, 3035 Arlington Avenue,
Toledo, OH 43614-5804, USA.
The presence of blood pressure (BP) quantitative trait loci (QTL) on rat
chromosome 10 has been clearly demonstrated by linkage analysis and substitution
mapping. Using congenic strains containing the LEW rat chromosomal segments on
the Dahl salt-sensitive (S) rat background, further iterations of congenic
substrains were constructed and characterized to fine-map a chromosome 10 region
(QTL1) linked to blood pressure. Comparison of seven congenic substrains refined
QTL1 to a 1.17 Mb segment flanked by D10Mco88 and D10Mco89, which are located at
71,513,116 and 72,684,774 bp, respectively. The newly defined QTL1, containing 18
genes, is captured in its entirety within a single congenic substrain. A thorough
transcript analysis revealed that 3 of these 18 genes, Ccl5, Ddx52, and
RGD1559577, had nonsynonymous allelic variations between the S rat and the LEW
rat. None of the detected transcripts within the newly defined QTL1 are
implicated directly in BP control in humans or model organisms. Therefore, the
present work defines a novel blood pressure QTL with three potential quantitative
trait nucleotides.
PMCID: PMC1808207
PMID: 17218081 [PubMed - indexed for MEDLINE]
PUBMED: 17218081
Find other GeneSets from this publication
GeneWeaver