DESCRIPTION:

QTL Associated with Blood pressure. On Chromosome 9 with a LOD score= , p-value =. From a(n) of

LABEL:

QTL-Bp284-Rat-Chr 9

SCORE TYPE:

Binary

DATE ADDED:

2015-06-10

DATE UPDATED:

2024-04-25

SPECIES:

AUTHORS:

Garrett MR, Meng H, Rapp JP, Joe B

TITLE:

Locating a blood pressure quantitative trait locus within 117 kb on the rat genome: substitution mapping and renal expression analysis.

JOURNAL:

Hypertension None None, Vol 45, pp. 451-9

ABSTRACT:

1. Hypertension. 2005 Mar;45(3):451-9. Epub 2005 Jan 17. Locating a blood pressure quantitative trait locus within 117 kb on the rat genome: substitution mapping and renal expression analysis. Garrett MR(1), Meng H, Rapp JP, Joe B. Author information: (1)Department of Physiology and Cardiovascular Genomics, Medical College of Ohio, Toledo 43614-5804, USA. Comment in Hypertension. 2005 Nov;46(5):e18; author reply e18-9. Previously, a blood pressure (BP) quantitative trait locus (QTL) on rat chromosome 9 (RNO9) was localized to a <2.4 cM interval using congenic strains generated by introgressing segments of RNO9 from the Dahl salt-resistant (R) rat into the background of the Dahl salt-sensitive (S) rat. Renal gene expression using Affymetrix gene chips was profiled on S and a congenic strain spanning the 2.4-cM BP QTL interval. This analysis identified 20 differentially expressed genes/expressed sequence tags. Of these, the locus with the greatest differential expression (30- to 35-fold) was regulated endocrine-specific protein 18 (Resp18), which also mapped in the 2.4-cM BP QTL interval. Additional substitution mapping located the QTL to <0.4 cM or approximately 493 kb. This newly defined QTL region still included Resp18. Nucleotide variants were identified between S and R genomic DNA of Resp18 in the coding, 5' regulatory and 3' untranslated regions. The coding sequence variation (T/C) occurs in exon 2 and predicts an amino acid change (Ile/Val) in the protein product. Resp18 was considered a differentially expressed positional candidate for the QTL. To fine-map the BP QTL, we constructed a congenic strain with a smaller introgressed region. Compared with the S rat, this strain (1) had significantly lower BP, (2) did not contain the R form of Resp18, and (3) did not retain the rather spectacular differential expression of Resp18. Together, these results demonstrate that a BP QTL independent of Resp18 exists within the newly defined 117-kb QTL region on RNO9. PMID: 15655120 [PubMed - indexed for MEDLINE] PUBMED: 15655120
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