1. Arterioscler Thromb Vasc Biol. 2002 Dec 1;22(12):2072-9.
Quantitative trait loci influencing blood and liver cholesterol concentration in
rats.
Bonné AC(1), den Bieman MG, Gillissen GF, Lankhorst A, Kenyon CJ, van Zutphen BF,
van Lith HA.
Author information:
(1)Department of Laboratory Animal Science, Faculty of Veterinary Medicine,
Utrecht University, Utrecht, The Netherlands. a.bonne@antrg.azn.nl
OBJECTIVE: The LEW/OlaHsd and BC/CpbU rat inbred strains differ markedly in blood
and hepatic cholesterol levels before and after a cholesterol-rich diet. To
define loci controlling these traits and related phenotypes, an F2 population
derived from these strains was genetically analyzed.
METHODS AND RESULTS: For each of the 192 F2 animals, phenotypes were determined,
and genomic DNA was screened for polymorphic microsatellite markers. Significant
quantitative trait loci (QTLs) were detected for basal serum cholesterol level on
chromosome 1 (D1Rat335-D1Rat27: total population, lod score 9.6; females, lod
score 10.3) and chromosome 7 (D7Rat69: males, lod score 4.1), for postdietary
serum cholesterol level on chromosome 2 (D2Rat69: total population, lod score
4.4) and chromosome 16 (D16Rat6-D16Rat44: total population, lod score 3.3), for
postdietary serum phospholipid level on chromosome 11 (D11Rat10: total
population, lod score 4.1; females, lod score 3.6), and for postdietary serum
aldosterone level on chromosome 1 (D1Rat14: females, lod score 3.7) and
chromosome 18 (D18Rat55-D18Rat8: females, lod score 2.9). In addition, QTLs with
borderline significance were found on chromosomes 3, 5 to 11, 15, and 18.
CONCLUSIONS: QTLs involved in blood and/or hepatic cholesterol concentrations (or
related phenotypes) in the rat were identified. This contributes to the value of
the rat as an animal model in studies researching the role of cholesterol in the
pathogenesis of atherosclerosis and other cholesterol-related diseases.
PMID: 12482837 [PubMed - indexed for MEDLINE]
PUBMED: 12482837
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