DESCRIPTION:

QTL Associated with Body weight. On Chromosome 1 with a LOD score= , p-value =0.05. From a(n) of

LABEL:

QTL-Bw54-Rat-Chr 1

SCORE TYPE:

Binary

DATE ADDED:

2015-06-10

DATE UPDATED:

2024-04-25

SPECIES:

AUTHORS:

Muramatsu Y, Yamada T, Taniguchi Y, Ogino T, Kose H, Matsumoto K, Sasaki Y

TITLE:

Pnlip encoding pancreatic lipase is possible candidate for obesity QTL in the OLETF rat.

JOURNAL:

Biochemical and biophysical research communications None None, Vol 331, pp. 1270-6

ABSTRACT:

1. Biochem Biophys Res Commun. 2005 Jun 17;331(4):1270-6. Pnlip encoding pancreatic lipase is possible candidate for obesity QTL in the OLETF rat. Muramatsu Y(1), Yamada T, Taniguchi Y, Ogino T, Kose H, Matsumoto K, Sasaki Y. Author information: (1)Department of Food Sciences, Shizuoka Eiwa Gakuin University Junior College, Ikeda, Shizuoka 422-8005, Japan. The Otsuka Long-Evans Tokushima Fatty (OLETF) rat exhibits polygenic obesity, and one of quantitative trait loci (QTLs) responsible for a susceptibility to obesity in the OLETF, Nidd6/of, has been mapped to the approximately 10-cM genomic region between D1Rat166 and D1Rat90 on chromosome 1 in (OLETF x normal) F2 intercross. In this study, we have attempted to identify the causal gene for the Nidd6/of QTL. A Nidd6/of congenic strain, constructed by introgressing the OLETF allele on the mapped Nidd6/of region in the normal F344 rat strain, confirmed the existence of the Nidd6/of as obesity QTL. The Nidd6/of region was refined to a approximately 2.3-cM genomic region between D1Rat225 and D1Rat90, using informative recombinants selected from (Nidd6/of congenic x F344) F1 x Nidd6/of congenic backcross progenies. Among 46 genes located within the approximately 2.3-cM region, pancreatic lipase gene, Pnlip, was regarded as the most prominent and physiologically relevant positional candidate for the Nidd6/of QTL. We found that Pnlip possesses an OLETF allele-specific increase of mRNA levels in the pancreas, and that the OLETF allele is longer in variable number of tandem repeat (VNTR) within the 5'-flanking region than normal alleles. We further showed that the Nidd6/of QTL completely cosegregates with Pnlip VNTR in the informative recombinants from (Nidd6/of congenic x F344) F1 x Nidd6/of congenic backcross progenies. These results suggest that Pnlip is possible candidate for the Nidd6/of QTL. PMID: 15883013 [PubMed - indexed for MEDLINE] PUBMED: 15883013
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