DESCRIPTION:

The intersection between the Pax6-null time series and Math1-null down-regulated microarray dataset produced 21 genes common between the two dataset. The 21 genes represent converging set of genes that are under the downstream of the transcription factor Pax6 and Math1 and their effect on cerebellar development.

LABEL:

Pax6 and Math1 Overlap

SCORE TYPE:

Binary

DATE ADDED:

None

DATE UPDATED:

2024-04-25

SPECIES:

AUTHORS:

Ha TJ, Swanson DJ, Kirova R, Yeung J, Choi K, Tong Y, Chesler EJ, Goldowitz D

TITLE:

Genome-wide microarray comparison reveals downstream genes of Pax6 in the developing mouse cerebellum.

JOURNAL:

The European journal of neuroscience Oct 2012, Vol 36, pp. 2888-98

ABSTRACT:

The Pax6 transcription factor is expressed in cerebellar granule cells and when mutated, as in the Sey/Sey mouse, produces granule cells with disturbed survival and migration and with defects in neurite extension. The impact of Pax6 on other genes in the context of cerebellar development has not been identified. In this study, we performed transcriptome comparisons between wildtype and Pax6-null whole cerebellar tissue at embryonic day (E) 13.5, 15.5 and 18.5 using Affymetrix arrays (U74Av2). Statistical analyses identified 136 differentially regulated transcripts (FDR 0.05, 1.2-fold change cutoff) over time in Pax6-null cerebellar tissue. In parallel we examined the Math1-null granuloprival cerebellum and identified 228 down-regulated transcripts (FDR 0.05, 1.2-fold change cutoff). The intersection of these two microarray datasets produced a total of 21 differentially regulated transcripts. For a subset of the identified transcripts, we used qRT-PCR to validate the microarray data and demonstrated the expression in the rhombic lip lineage and differential expression in Pax6-null cerebellum with in situ hybridisation analysis. The candidate genes identified in this way represent direct or indirect Pax6-downstream genes involved in cerebellar development. PUBMED: 22817342
Find other GeneSets from this publication