GeneSet Information

Tier III GS14931 • Upregulated gene expression of PKC-gamma mutant mice due to chronic ethanol diet

DESCRIPTION:

Study shows that PKC-gamma wild-type mice develop tolerance to the sedative- hypnotic effects of ethanol after chronic ethanol treatment but mutant mice do not, making these genotypes a suitable model for identifying changes in gene expression related developing tolerance toward ethanol. This gene set comprises 27 ethanol- dependence genes that were upregulated in the PKC-gamma wild-type mice during the experiment.

LABEL:

UpRgEtOH PKCgamma mutnt

SCORE TYPE:

Binary

DATE ADDED:

2009-01-20

DATE UPDATED:

2024-10-22

SPECIES:

AUTHORS:

Bowers BJ, Radcliffe RA, Smith AM, Miyamoto-Ditmon J, Wehner JM

TITLE:

Microarray analysis identifies cerebellar genes sensitive to chronic ethanol treatment in PKCgamma mice.

JOURNAL:

Alcohol (Fayetteville, N.Y.) Aug 2006, Vol 40, pp. 19-33

ABSTRACT:

Neuroadaptive changes that occur in the development of ethanol tolerance may be the result of alterations in gene expression. We have shown that PKCgamma wild-type mice develop tolerance to the sedative-hypnotic effects of ethanol after chronic ethanol treatment; whereas, mutant mice do not, making these genotypes a suitable model for identifying changes in gene expression related to tolerance development. Using a two-stage process, several genes were initially identified using microarray analyses of cerebellar tissue from ethanol-treated PKCgamma mutant and wild-type mice. Subsequent confirmation of a subset of these genes using quantitative real time reverse transcriptase polymerase chain reactions (qRT-PCR) was done to verify gene expression changes. A total of 109 genes from different functional classifications were identified in these groups on the microarrays. Eight genes were selected for verification as follows: three, Twik-1, Plp, and Adk2, were chosen as genes related to tolerance; another three, Hsp70.2, Bdnf, and Th, were chosen as genes related to resistance to tolerance; and two genes, JunB and Nur77, were selected as candidate genes sensitive to chronic ethanol. The results from the verification experiments indicated that Twik-1, which codes for a potassium channel, was associated with tolerance and appeared to be dependent on the presence of PKCgamma. No genes were confirmed to be related to resistance to tolerance; however, expression of two of these, Hsp70.2 and Th, were found to be sensitive to chronic ethanol and were added to the transcription factors, JunB and Nur77, confirmed by qRT-PCR, as a subset of genes that respond to chronic ethanol. PUBMED: 17157717
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Annotation Information

No sequence read archive data associated with this GeneSet.


Gene Expression Regulation (D005786)
Nuclear Receptor Subfamily 4, Group A, Member 1 (D057105)
Animals (D000818)
Reproducibility of Results (D015203)
Polymerase Chain Reaction (D016133)
Mice (D051379)
Gene Expression Profiling (D020869)
Potassium (D011188)
Protein Kinase C (D011493)
Myelin Proteolipid Protein (D018991)
Microarray Analysis (D046228)
RNA, Messenger (D012333)
Central Nervous System Depressants (D002492)
HSP70 Heat-Shock Proteins (D018840)
Mice, Knockout (D018345)
Proto-Oncogene Proteins c-jun (D016755)
Therapeutics (D013812)
Classification (D002965)
Potassium Channels, Tandem Pore Domain (D024683)
Potassium Channels (D015221)
Time (D013995)
Reverse Transcriptase Polymerase Chain Reaction (D020133)
Genotype (D005838)
Tyrosine 3-Monooxygenase (D014446)
Drug Tolerance (D004361)
Tissues (D014024)
Receptors, Cytoplasmic and Nuclear (D018160)
RNA-Directed DNA Polymerase (D012194)
Oligonucleotide Array Sequence Analysis (D020411)
Mice, Inbred C57BL (D008810)
Transcription Factors (D014157)
Nerve Tissue Proteins (D009419)
Cerebellum (D002531)
DNA-Directed RNA Polymerases (D012321)
Brain-Derived Neurotrophic Factor (D019208)
Hypnotics and Sedatives (D006993)
DNA-Binding Proteins (D004268)
Set (Psychology) (D012718)
Diet (D004032)
Alcoholism (D000437)
Ethanol (D000431)
Receptors, Steroid (D011987)
no abnormal phenotype detected (MP:0002169)
gene expression (GO:0010467)

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Supported by NIH R01 AA18776 jointly funded by NIAAA and NIDA, and JAX Center for Precision Genetics NIH U54 OD 020351

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