DESCRIPTION:

Cerebellum B6J vs B6C differences between substrains that correspond to a 5% FDR. A small number of genes are highly statistically significantly divergent between B6 substrains, B6J (high consumption preference) and B6C (low consumption preference). Fold expression change are relative to B6J.

LABEL:

Cerebellum B6J vs B6C

SCORE TYPE:

Effect

DATE ADDED:

2011-06-02

DATE UPDATED:

2024-04-25

SPECIES:

AUTHORS:

Mulligan MK, Ponomarev I, Boehm SL 2nd, Owen JA, Levin PS, Berman AE, Blednov YA, Crabbe JC, Williams RW, Miles MF, Bergeson SE

TITLE:

Alcohol trait and transcriptional genomic analysis of C57BL/6 substrains.

JOURNAL:

Genes, brain, and behavior Aug 2008, Vol 7, pp. 677-89

ABSTRACT:

C57BL/6 inbred mice have been widely used as research models; however, widespread demand has led to the creation of several B6 substrains with markedly different phenotypes. In this study, we report that two substrains of C57BL/6 mice, C57BL/6J (B6J) and C57BL/6NCrl (B6C), separated over 50 years ago at two different breeding facilities differ significantly in alcohol consumption and alcohol preference. The genomes of these two substrains are estimated to differ by only 1-2% of all gene loci, providing a unique opportunity to extract particular expression signatures between these substrains that are associated with quantifiable behavioral differences. Expression profiling of the cortex and striatum, hippocampus, cerebellum and the ventral brain region from alcohol-naïve B6C and B6J mice showed intervals on three chromosomes that are enriched in clusters of coregulated transcripts significantly divergent between the substrains. Additional analysis identified two genomic regions containing putative copy number differences between the substrains. One such region on chromosome 14 contained an estimated 3n copy number in the B6J genome compared with B6C. Within this interval, a gene of unknown function, D14Ertd449e, was found to be both associated with alcohol preference and vary in copy number across several inbred strain lineages. H2afz, Psen1, Wdfy1 and Clu were also identified as candidate genes that may be involved in influencing alcohol consumption. PUBMED: 18397380
Find other GeneSets from this publication