GeneSet Information

Tier III GS1249 • Gene expression changes in both PKCgamma mutant and wild-type mice due to chronic ethanol diet

DESCRIPTION:

Using a two-stage process, several genes were initially identified using microarray analyses of cerebellar tissue from ethanol-treated PKCgamma mutant and wild-type mice. This geneset consists of genes related to PKCgamma mutant expression changes due to chronic ethanol diet.

LABEL:

DiffExpPKCgWTMTChronEtoH

SCORE TYPE:

Binary

DATE ADDED:

2008-04-22

DATE UPDATED:

2024-04-25

SPECIES:

AUTHORS:

Bowers BJ, Radcliffe RA, Smith AM, Miyamoto-Ditmon J, Wehner JM

TITLE:

Microarray analysis identifies cerebellar genes sensitive to chronic ethanol treatment in PKCgamma mice.

JOURNAL:

Alcohol (Fayetteville, N.Y.) Aug 2006, Vol 40, pp. 19-33

ABSTRACT:

Neuroadaptive changes that occur in the development of ethanol tolerance may be the result of alterations in gene expression. We have shown that PKCgamma wild-type mice develop tolerance to the sedative-hypnotic effects of ethanol after chronic ethanol treatment; whereas, mutant mice do not, making these genotypes a suitable model for identifying changes in gene expression related to tolerance development. Using a two-stage process, several genes were initially identified using microarray analyses of cerebellar tissue from ethanol-treated PKCgamma mutant and wild-type mice. Subsequent confirmation of a subset of these genes using quantitative real time reverse transcriptase polymerase chain reactions (qRT-PCR) was done to verify gene expression changes. A total of 109 genes from different functional classifications were identified in these groups on the microarrays. Eight genes were selected for verification as follows: three, Twik-1, Plp, and Adk2, were chosen as genes related to tolerance; another three, Hsp70.2, Bdnf, and Th, were chosen as genes related to resistance to tolerance; and two genes, JunB and Nur77, were selected as candidate genes sensitive to chronic ethanol. The results from the verification experiments indicated that Twik-1, which codes for a potassium channel, was associated with tolerance and appeared to be dependent on the presence of PKCgamma. No genes were confirmed to be related to resistance to tolerance; however, expression of two of these, Hsp70.2 and Th, were found to be sensitive to chronic ethanol and were added to the transcription factors, JunB and Nur77, confirmed by qRT-PCR, as a subset of genes that respond to chronic ethanol. PUBMED: 17157717
Find other GeneSets from this publication

Annotation Information

No sequence read archive data associated with this GeneSet.


Gene Expression Regulation (D005786)
Nuclear Receptor Subfamily 4, Group A, Member 1 (D057105)
Animals (D000818)
Reproducibility of Results (D015203)
Polymerase Chain Reaction (D016133)
Mice (D051379)
Gene Expression Profiling (D020869)
Potassium (D011188)
Protein Kinase C (D011493)
Myelin Proteolipid Protein (D018991)
Microarray Analysis (D046228)
RNA, Messenger (D012333)
Central Nervous System Depressants (D002492)
HSP70 Heat-Shock Proteins (D018840)
Mice, Knockout (D018345)
Proto-Oncogene Proteins c-jun (D016755)
Therapeutics (D013812)
Classification (D002965)
Potassium Channels, Tandem Pore Domain (D024683)
Potassium Channels (D015221)
Time (D013995)
Reverse Transcriptase Polymerase Chain Reaction (D020133)
Genotype (D005838)
Tyrosine 3-Monooxygenase (D014446)
Drug Tolerance (D004361)
Tissues (D014024)
Receptors, Cytoplasmic and Nuclear (D018160)
RNA-Directed DNA Polymerase (D012194)
Oligonucleotide Array Sequence Analysis (D020411)
Mice, Inbred C57BL (D008810)
Transcription Factors (D014157)
Nerve Tissue Proteins (D009419)
Cerebellum (D002531)
DNA-Directed RNA Polymerases (D012321)
Brain-Derived Neurotrophic Factor (D019208)
DNA-Binding Proteins (D004268)
Diet (D004032)
Alcoholism (D000437)
Ethanol (D000431)
Receptors, Steroid (D011987)
no abnormal phenotype detected (MP:0002169)
gene expression (GO:0010467)

Gene List • 9 Genes

Uploaded As Gene Symbol Homology Score Priority LinkOuts Emphasis  

Manage GeneSets

Analyze GeneSets

Manage Accounts

GeneWeaver Links

Policies


Supported by NIH R01 AA18776 jointly funded by NIAAA and NIDA, and JAX Center for Precision Genetics NIH U54 OD 020351