DESCRIPTION:

Ethanol Preference from BXD lines span 58586243-108586243. This interval was obtained by using an arbitrary interval width of 25 Mbp around the peak marker (Build 37, MGI, http://informatics.jax.org). Marker Loci associated with 10% Ethanol Preferences Drinking at p<0.05 (Two Tailed) in the BXD RI set and the Correlation Coefficient, p and Estimated LOD. D15Mit33 (83586243 NCBI 37) p=0.05, LOD=0.08 overall LOD BXD & Select Line 2.4.

LABEL:

QTL-Mouse-Chr 15

SCORE TYPE:

Binary

DATE ADDED:

2011-04-11

DATE UPDATED:

2020-05-06

SPECIES:

AUTHORS:

Belknap JK, Richards SP, O\'Toole LA, Helms ML, Phillips TJ

TITLE:

Short-term selective breeding as a tool for QTL mapping: ethanol preference drinking in mice.

JOURNAL:

Behavior genetics Jan 1997, Vol 27, pp. 55-66

ABSTRACT:

Short-term selective breeding starting from an F2 intercross of two inbred strains is a largely unexploited but potentially useful tool for quantitative trait locus (QTL) mapping. The selection lines can also serve as a valuable confirmation test of recombinant inbred (RI) QTL results when the same two progenitor strains are used. Starting from an F2 from a C57BL/6J (B6) X DBA/2J (D2) cross (B6D2F2), this approach was used in a population of approximately 72 mice per generation bidirectionally selected for two-bottle choice 10% ethanol (alcohol) preference for four generations. The high-preference line diverged significantly from the low line in the first generation with a realized heritability of .32. By generation 4, the preference ratios in the high line were double those seen in the low line. Regions of the genome previously implicated by BXD RI QTL analysis as containing QTLs were searched using microsatellite markers. The test for the presence of QTLs was based on the divergence of marker allele frequencies in the two oppositely selected lines significantly exceeding that expected from random (genetic) drift and allele frequency estimation error. Combining the BXD and two-way selection line results, the most probable QTL was found on chromosome 3 (near the adhl locus; LOD approximately 2.9), other probable QTLs were found with LOD 2.4-2.6. PUBMED: 9145544
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