This gene set contains 33 genes that showed greater expression in C57BL/6 (B6) than in C57BL/6 (B6) mice when Microarray Mouse Expression Array 430A analysis was used. Background: Study integrated five different ethanol-associated QTL (chr1, chr2, chr4, chr9 or chr19) and gene expression data of B6 and D2 inbred mouse strains with the goal of accelerating movement from QTLs to the QTGs (Quantitative Trait Gene).
This gene set contains 18 genes that showed greater expression in C57BL/6 (B6) than in mice DBA/2 (D2) when Microarray mouse genome U74A V2 analysis was used. Background: Study integrated five different ethanol-associated QTL (chr1, chr2, chr4, chr9 or chr19) and gene expression data of B6 and D2 inbred mouse strains with the goal of accelerating movement from QTLs to the QTGs (Quantitative Trait Gene).
Activation of the mesolimbic dopamine reward pathway by acute ethanol produces reinforcement and changes in gene expression that appear to be crucial to the molecular basis for adaptive behaviors and addiction. The inbred mouse strains DBA/2J and C57BL/6J exhibit contrasting acute behavioral responses to ethanol. We used oligonucleotide microarrays and bioinformatics methods to characterize patterns of gene expression in three brain regions of the mesolimbic reward pathway of these strains. Expression profiling included examination of both differences in gene expression 4 h after saline injection or acute ethanol (2 g/kg). Using a rigorous stepwise method for microarray analysis, we identified 788 genes differentially expressed in control DBA/2J versus C57BL/6J mice and 307 ethanol-regulated genes in the nucleus accumbens, prefrontal cortex, and ventral tegmental area. There were strikingly divergent patterns of ethanol-responsive gene expression in the two strains. Ethanol-responsive genes also showed clustering at discrete chromosomal regions, suggesting local chromatin effects in regulation. Ethanol-regulated genes were generally related to neuroplasticity, but regulation of discrete functional groups and pathways was brain region specific: glucocorticoid signaling, neurogenesis, and myelination in the prefrontal cortex; neuropeptide signaling and developmental genes, including factor Bdnf, in the nucleus accumbens; and retinoic acid signaling in the ventral tegmental area. Bioinformatics analysis identified several potential candidate genes for quantitative trait loci linked to ethanol behaviors, further supporting a role for expression profiling in identifying genes for complex traits. Brain region-specific changes in signaling and neuronal plasticity may be critical components in development of lasting ethanol behavioral phenotypes such as dependence, sensitization, and craving.
Study integrated five different ethanol-associated QTL (chr1, chr2, chr4, chr9 or chr19) and gene expression data of C57BL/6 (B6) and DBA/2 (D2) inbred mouse strains with the goal of accelerating movement from QTLs to the QTGs (Quantitative Trait Gene). This gene set contains 28 genes that showed greater expression in D2 than in B6 mice when Microarray Mouse Expression Array 430A analysis was used.
This gene set contains 11 genes that showed greater expression in DBA/2 (D2) than in C57BL/6 (B6) mice when Microarray mouse genome U74A V2 analysis was used. Background: Study integrated five different ethanol-associated QTL (chr1, chr2, chr4, chr9 or chr19) and gene expression data of B6 and D2 inbred mouse strains with the goal of accelerating movement from QTLs to the QTGs (Quantitative Trait Gene).
A list of the 307 genes found to be upregulated or downregulated by ethanol in PFC, VTA or NA of B6 or D2 mice. ID number represents cluster membership from Figure 4.
Authors:
Kerns RT, Ravindranathan A, Hassan S, Cage MP, York T, Sikela JM, Williams RW, Miles MF
Hippocampus Gene Expression Correlates for HARGREAVES_MEANBOTH measured in BXD RI Females & Males obtained using GeneNetwork Hippocampus Consortium M430v2 (Jun06) RMA. The HARGREAVES_MEANBOTH measures Thermal Nociception Hargreaves' Test under the domain Pain. The correlates were thresholded at a p-value of less than 0.001.
Authors:
Philip VM, Duvvuru S, Gomero B, Ansah TA, Blaha CD, Cook MN, Hamre KM, Lariviere WR, Matthews DB, Mittleman G, Goldowitz D, Chesler EJ
Striatum Gene Expression Correlates for ENTRIES_OPEN measured in BXD RI Females & Males obtained using GeneNetwork Striatum M430V2 (Apr05) RMA. The ENTRIES_OPEN measures Number of entries into open arms of plus maze under the domain Ethanol. The correlates were thresholded at a p-value of less than 0.001.
Authors:
Philip VM, Duvvuru S, Gomero B, Ansah TA, Blaha CD, Cook MN, Hamre KM, Lariviere WR, Matthews DB, Mittleman G, Goldowitz D, Chesler EJ
Whole Brain Gene Expression Correlates for LM_CUE_SUPPRESS measured in BXD RI Males obtained using INIA Brain mRNA M430 (Jun06) RMA. The LM_CUE_SUPPRESS measures Suppression of activity in altered context under the domain Basal Behavior. The correlates were thresholded at a p-value of less than 0.001.
Authors:
Philip VM, Duvvuru S, Gomero B, Ansah TA, Blaha CD, Cook MN, Hamre KM, Lariviere WR, Matthews DB, Mittleman G, Goldowitz D, Chesler EJ
Cerebellum Gene Expression Correlates for NEVCOUNT15 measured in BXD RI Males obtained using SJUT Cerebellum mRNA M430 (Mar05) RMA. The NEVCOUNT15 measures Novel environment vertical activity counts min 0-15 under the domain Morphine. The correlates were thresholded at a p-value of less than 0.001.
Authors:
Philip VM, Duvvuru S, Gomero B, Ansah TA, Blaha CD, Cook MN, Hamre KM, Lariviere WR, Matthews DB, Mittleman G, Goldowitz D, Chesler EJ
Neocortex Gene Expression Correlates for HAND_6HOURS measured in BXD RI Females & Males obtained using GeneNetwork Neocortex ILM6v1.1 (Feb08) RankInv. The HAND_6HOURS measures Handling induced convulsions 6 hrs after ethanol under the domain Ethanol HIC. The correlates were thresholded at a p-value of less than 0.001.
Authors:
Philip VM, Duvvuru S, Gomero B, Ansah TA, Blaha CD, Cook MN, Hamre KM, Lariviere WR, Matthews DB, Mittleman G, Goldowitz D, Chesler EJ
Neocortex Gene Expression Correlates for OF_REAR_5_10 measured in BXD RI Females obtained using GeneNetwork Neocortex ILM6v1.1 (Feb08) RankInv. The OF_REAR_5_10 measures Open Field - Total rears 5-10 minutes under the domain Basal Behavior. The correlates were thresholded at a p-value of less than 0.001.
Authors:
Philip VM, Duvvuru S, Gomero B, Ansah TA, Blaha CD, Cook MN, Hamre KM, Lariviere WR, Matthews DB, Mittleman G, Goldowitz D, Chesler EJ
Neocortex Gene Expression Correlates for OF_REARS measured in BXD RI Females obtained using GeneNetwork Neocortex ILM6v1.1 (Feb08) RankInv. The OF_REARS measures Open Field - Total number of Rears under the domain Basal Behavior. The correlates were thresholded at a p-value of less than 0.001.
Authors:
Philip VM, Duvvuru S, Gomero B, Ansah TA, Blaha CD, Cook MN, Hamre KM, Lariviere WR, Matthews DB, Mittleman G, Goldowitz D, Chesler EJ
Striatum Gene Expression Correlates for PCT_ENTRIES_OPEN measured in BXD RI Females & Males obtained using GeneNetwork Striatum M430V2 (Apr05) RMA. The PCT_ENTRIES_OPEN measures Percentage of entries into open arms of plus maze under the domain Porsolt. The correlates were thresholded at a p-value of less than 0.001.
Authors:
Philip VM, Duvvuru S, Gomero B, Ansah TA, Blaha CD, Cook MN, Hamre KM, Lariviere WR, Matthews DB, Mittleman G, Goldowitz D, Chesler EJ
These genes are a 1 class SAM significant (1% FDR) in nucleus accumbens (core + shell) for saline treated ("basal") control vs. Fyn KO mice. The list was filtered for an average Sscore >2.0 or <-2.0. Data from Farris and Miles, PLoS One, 2013.
cocaine related behavior 5 (Cocrb5) spans 57.11978 - 107.11978 Mbp (NCBI Build 37) on Chr 4. Obtained from MGI (http://www.informatics.jax.org) by searching for QTLs containing the keyword .
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