Differential gene expression in nucleus accumbens somatostatin interneurons_cocaine_mice_pvalue
Description:
To characterize transcriptional alterations that cocaine induces in these cells, we perform cell type-specific RNA-sequencing on FACS-isolated nuclei of somatostatin interneurons and identified 1100 DETs enriched for processes related to neural plasticity. To profile the entire (non poly-A selected) transcriptome of NAc somatostatin interneurons, we generated a transgenic reporter line (SST-TLG498 mice) to label the nuclei of these cells with a modified form of EGFP that is retained in the nuclear membrane (EGFP-F)22, enabling their isolation from NAc dissections using FACS. We succeeded in FACS-isolating nuclei suitable for RNA-sequencing from individual SST-TLG498 mice. We proceeded with differential expression analysis of the RNA-sequencing data to identify differentially expressed transcripts (DETs) in NAc somatostatin interneurons in response to repeated cocaine exposure: 778 transcripts were upregulated by cocaine and 322 were downregulated.
Authors:
Efrain A Ribeiro, Marine Salery, Joseph R Scarpa, Erin S Calipari, Peter J Hamilton, Stacy M Ku, Hope Kronman, Immanuel Purushothaman, Barbara Juarez, Mitra Heshmati, Marie Doyle, Casey Lardner, Dominicka Burek, Ana Strat, Stephen Pirpinias, Ezekiell Mouzon, Ming-Hu Han, Rachael L Neve, Rosemary C Bagot, Andrew Kasarskis, Ja Wook Koo, Eric J Nestler
QTL Associated with Bone mineral density. On Chromosome 19 with a LOD score= 12.1, p-value =1.00E-04. From a(n) of QTL Associated with Bone mineral density. On Chromosome 10 with a LOD score= 9.4, p-value =1.00E-04. From a(n) of
Authors:
Koller DL, Alam I, Sun Q, Liu L, Fishburn T, Carr LG, Econs MJ, Foroud T, Turner CH
QTL Associated with Cardiac mass. On Chromosome 10 with a LOD score= 4.55, p-value =. From a(n) intercross of
Authors:
Bilusić M, Moreno C, Barreto NE, Tschannen MR, Harris EL, Porteous WK, Thompson CM, Grigor MR, Weder A, Boerwinkle E, Hunt SC, Curb JD, Jacob HJ, Kwitek AE
QTL Associated with Bone mineral density. On Chromosome 10 with a LOD score= 4.9, p-value =1.00E-04. From a(n) intercross of
Authors:
Koller DL, Liu L, Alam I, Sun Q, Econs MJ, Foroud T, Turner CH
Add Selected GeneSets to Project(s)
Warning: You are not signed in. Adding these genesets to a project will create a guest account for you.
Guest accounts are temporary, and will be removed within 24 hours of creation. Guest accounts can be registered as full accounts, but you cannot associate a guest account with an existing account.
If you already have an account, you should sign into that account before proceeding.