Ethanol Induced Hypothermia Chr# 7 rs13479153(25722935) with right flanking marker rs3700068(4187548) and left marker rs3716088(140189839). This was mapped in 300 + (b6x129)F2 mice.
BECs at LORR Recovery Chr# 7 rs13479145(19988355) with right flanking marker rs6384973(5036805) and left marker rs3663313 (63388111). This was mapped in 300 + (b6x129)F2 mice.
Average rotarod training latency Chr# 7 mCV23423763(68111945) with right flanking marker rs3700068(4187548) and left marker rs3663988(146505067). This was mapped in 300 + (b6x129)F2 mice.
Adolescent D2 transcripts sig. altered in PFC using S-score analysis at FDR < 0.05 (EtOH vs control)
Description:
DBA/2J males and females (n = 24/sex) were orally dosed with 4 g/kg ethanol (25% w/v in water by gavage) or water intermittently (2 days on/2 days off) on PND 29, 30, 33, 34, 37, 38, 41, and 42. Tissue was collected for gene expression studies at PND 43 (n = 22) and PND 66 (n = 19). Behaviorally naïve tissue from the PFC was collected 24 h (at PND 43) and 3 weeks (at PND 66) after the last ethanol binge (dose). Total RNA was analyzed for gene-level expression differences using Mouse Transcriptome Arrays v1.0. We performed an analysis using the S-score probe-level algorithm which we have previously shown to have increased sensitivity for differential expression analysis (Zhang et al., 2002; Kennedy et al., 2006). For this analysis, data was collapsed over sex to increase the power to detect differences between ethanol treatment versus controls and to focus on lasting differences following binge ethanol. To assess genes that were persistently regulated long-term following adolescent binge ethanol, we intersected the S-score analysis gene list significantly altered by ethanol in adolescents with the list obtained from adults.
Authors:
Jennifer T Wolstenholme, Tariq Mahmood, Guy M Harris, Shahroze Abbas, Michael F Miles
Adult D2 transcripts sig. altered using S-score analysis at FDR < 0.05 (EtOH vs control)
Description:
DBA/2J males and females (n = 24/sex) were orally dosed with 4 g/kg ethanol (25% w/v in water by gavage) or water intermittently (2 days on/2 days off) on PND 29, 30, 33, 34, 37, 38, 41, and 42. Tissue was collected for gene expression studies at PND 43 (n = 22) and PND 66 (n = 19). Behaviorally naïve tissue from the PFC was collected 24 h (at PND 43) and 3 weeks (at PND 66) after the last ethanol binge (dose). Total RNA was analyzed for gene-level expression differences using Mouse Transcriptome Arrays v1.0. We performed an analysis using the S-score probe-level algorithm which we have previously shown to have increased sensitivity for differential expression analysis (Zhang et al., 2002; Kennedy et al., 2006). For this analysis, data was collapsed over sex to increase the power to detect differences between ethanol treatment versus controls and to focus on lasting differences following binge ethanol. To assess genes that were persistently regulated long-term following adolescent binge ethanol, we intersected the S-score analysis gene list significantly altered by ethanol in adolescents with the list obtained from adults.
Authors:
Jennifer T Wolstenholme, Tariq Mahmood, Guy M Harris, Shahroze Abbas, Michael F Miles
Intersection of adolescent and adult transcripts sig. altered using S-score analysis at FDR < 0.05 (EtOH vs control)
Description:
DBA/2J males and females (n = 24/sex) were orally dosed with 4 g/kg ethanol (25% w/v in water by gavage) or water intermittently (2 days on/2 days off) on PND 29, 30, 33, 34, 37, 38, 41, and 42. Tissue was collected for gene expression studies at PND 43 (n = 22) and PND 66 (n = 19). Behaviorally naïve tissue from the PFC was collected 24 h (at PND 43) and 3 weeks (at PND 66) after the last ethanol binge (dose). Total RNA was analyzed for gene-level expression differences using Mouse Transcriptome Arrays v1.0. We performed an analysis using the S-score probe-level algorithm which we have previously shown to have increased sensitivity for differential expression analysis (Zhang et al., 2002; Kennedy et al., 2006). For this analysis, data was collapsed over sex to increase the power to detect differences between ethanol treatment versus controls and to focus on lasting differences following binge ethanol. To assess genes that were persistently regulated long-term following adolescent binge ethanol, we intersected the S-score analysis gene list significantly altered by ethanol in adolescents with the list obtained from adults.
Authors:
Jennifer T Wolstenholme, Tariq Mahmood, Guy M Harris, Shahroze Abbas, Michael F Miles
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