GeneSet Title
Tier III
Fly
511 Genes
GS75550
LABEL: morozova alcohol exposDESCRIPTION: From Morozova et al., 2006; List of genes differentially expressed between treatment groups with q<.05
Tier I
Fly
DRG
136 Genes
GS86456
LABEL: Chronic ethanol - Ethanol vs. ControlDESCRIPTION: Chronic ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee A list of probe sets differentially expressed between treatment groups with q < 0.05. These transcript levels show sustained altered expression after the first exposure to ethanol. (NIF Table ID 381 [217.5])
Tier I
Fly
DRG
8 Genes
GS86468
LABEL: Acute ethanol - Ethanol vs. ControlDESCRIPTION: Acute ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee Probe sets with altered transcriptional regulation after ethanol exposure with q < 0.001. These genes show increased expression after the first exposure to ethanol, but a return to control levels 2 hours later after the second exposure. (NIF Table ID 382 [222])
Tier I
Fly
DRG
315 Genes
GS86499
LABEL: Acute ethanol - Ethanol vs. ControlDESCRIPTION: Acute ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee A list of probe sets differentially expressed between treatment groups with q < 0.05. The transcript abundance only changes after the second exposure to alcohol. (NIF Table ID 381 [218.5])
Tier I
Fly
DRG
9 Genes
GS86594
LABEL: Chronic ethanol - Ethanol vs. ControlDESCRIPTION: Chronic ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee Probe sets with altered transcriptional regulation after ethanol exposure with q < 0.001. These transcripts show sustained altered expression after the first exposure to ethanol. (NIF Table ID 382 [220.5])
Tier I
Fly
DRG
15 Genes
GS86713
LABEL: Acute ethanol - Ethanol vs. ControlDESCRIPTION: Acute ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee Probe sets with altered transcriptional regulation after ethanol exposure with q < 0.001. These transcripts show altered expression only after the second exposure to alcohol. (NIF Table ID 382 [221])
Tier I
Fly
DRG
58 Genes
GS86719
LABEL: Chronic ethanol - Ethanol vs. ControlDESCRIPTION: Chronic ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee A list of probe sets differentially expressed between treatment groups with q < 0.05. Transcript levels are altered following the first exposure to ethanol, but are not sustained during the development of tolerance. (NIF Table ID 381 [219.5])
Tier I
Fly
DRG
15 Genes
GS86863
LABEL: Chronic ethanol - Ethanol vs. ControlDESCRIPTION: Chronic ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee Probe sets with altered transcriptional regulation after ethanol exposure with q < 0.001. These transcripts show altered expression only after the second exposure to alcohol. (NIF Table ID 382 [221.5])
Tier I
Fly
DRG
9 Genes
GS86905
LABEL: Acute ethanol - Ethanol vs. ControlDESCRIPTION: Acute ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee Probe sets with altered transcriptional regulation after ethanol exposure with q < 0.001. These transcripts show sustained altered expression after the first exposure to ethanol. (NIF Table ID 382 [220])
Tier I
Fly
DRG
315 Genes
GS87104
LABEL: Chronic ethanol - Ethanol vs. ControlDESCRIPTION: Chronic ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee A list of probe sets differentially expressed between treatment groups with q < 0.05. The transcript abundance only changes after the second exposure to alcohol. (NIF Table ID 381 [218])
Tier I
Fly
DRG
136 Genes
GS87106
LABEL: Acute ethanol - Ethanol vs. ControlDESCRIPTION: Acute ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee A list of probe sets differentially expressed between treatment groups with q < 0.05. These transcript levels show sustained altered expression after the first exposure to ethanol. (NIF Table ID 381 [217])
Tier I
Fly
DRG
8 Genes
GS87136
LABEL: Chronic ethanol - Ethanol vs. ControlDESCRIPTION: Chronic ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee Probe sets with altered transcriptional regulation after ethanol exposure with q < 0.001. These genes show increased expression after the first exposure to ethanol, but a return to control levels 2 hours later after the second exposure. (NIF Table ID 382 [222.5])
Tier I
Fly
DRG
58 Genes
GS87166
LABEL: Acute ethanol - Ethanol vs. ControlDESCRIPTION: Acute ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee A list of probe sets differentially expressed between treatment groups with q < 0.05. Transcript levels are altered following the first exposure to ethanol, but are not sustained during the development of tolerance. (NIF Table ID 381 [219])
Tier I
Fly
DRG
9 Genes
GS87347
LABEL: Acute ethanol - Ethanol vs. ControlDESCRIPTION: Acute ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee Probe sets with altered transcriptional regulation after ethanol exposure with q < 0.001. These transcripts show sustained altered expression after the first exposure to ethanol. (NIF Method ID 220)
Tier I
Fly
DRG
9 Genes
GS87348
LABEL: Chronic ethanol - Ethanol vs. ControlDESCRIPTION: Chronic ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee Probe sets with altered transcriptional regulation after ethanol exposure with q < 0.001. These transcripts show sustained altered expression after the first exposure to ethanol. (NIF Method ID 220.5)
Tier I
Fly
DRG
16 Genes
GS87349
LABEL: Acute ethanol - Ethanol vs. ControlDESCRIPTION: Acute ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee Probe sets with altered transcriptional regulation after ethanol exposure with q < 0.001. These transcripts show altered expression only after the second exposure to alcohol. (NIF Method ID 221)
Tier I
Fly
DRG
16 Genes
GS87350
LABEL: Chronic ethanol - Ethanol vs. ControlDESCRIPTION: Chronic ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee Probe sets with altered transcriptional regulation after ethanol exposure with q < 0.001. These transcripts show altered expression only after the second exposure to alcohol. (NIF Method ID 221.5)
Tier I
Fly
DRG
10 Genes
GS87351
LABEL: Acute ethanol - Ethanol vs. ControlDESCRIPTION: Acute ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee Probe sets with altered transcriptional regulation after ethanol exposure with q < 0.001. These genes show increased expression after the first exposure to ethanol, but a return to control levels 2 hours later after the second exposure. (NIF Method ID 222)
Tier I
Fly
DRG
10 Genes
GS87352
LABEL: Chronic ethanol - Ethanol vs. ControlDESCRIPTION: Chronic ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee Probe sets with altered transcriptional regulation after ethanol exposure with q < 0.001. These genes show increased expression after the first exposure to ethanol, but a return to control levels 2 hours later after the second exposure. (NIF Method ID 222.5)
Tier I
Fly
DRG
135 Genes
GS87353
LABEL: Acute ethanol - Ethanol vs. ControlDESCRIPTION: Acute ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee A list of probe sets differentially expressed between treatment groups with q < 0.05. These transcript levels show sustained altered expression after the first exposure to ethanol. (NIF Method ID 217)
Tier I
Fly
DRG
135 Genes
GS87354
LABEL: Chronic ethanol - Ethanol vs. ControlDESCRIPTION: Chronic ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee A list of probe sets differentially expressed between treatment groups with q < 0.05. These transcript levels show sustained altered expression after the first exposure to ethanol. (NIF Method ID 217.5)
Tier I
Fly
DRG
330 Genes
GS87355
LABEL: Chronic ethanol - Ethanol vs. ControlDESCRIPTION: Chronic ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee A list of probe sets differentially expressed between treatment groups with q < 0.05. The transcript abundance only changes after the second exposure to alcohol. (NIF Method ID 218)
Tier I
Fly
DRG
330 Genes
GS87356
LABEL: Acute ethanol - Ethanol vs. ControlDESCRIPTION: Acute ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee A list of probe sets differentially expressed between treatment groups with q < 0.05. The transcript abundance only changes after the second exposure to alcohol. (NIF Method ID 218.5)
Tier I
Fly
DRG
61 Genes
GS87357
LABEL: Acute ethanol - Ethanol vs. ControlDESCRIPTION: Acute ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee A list of probe sets differentially expressed between treatment groups with q < 0.05. Transcript levels are altered following the first exposure to ethanol, but are not sustained during the development of tolerance. (NIF Method ID 219)
Tier I
Fly
DRG
61 Genes
GS87358
LABEL: Chronic ethanol - Ethanol vs. ControlDESCRIPTION: Chronic ethanol - Ethanol vs. Control DNA microarray Change in gene expression Pre-equilibrated with ethanol vapor, flies were exposed in an inebriometer from which they were eluted at one minute intervals. The mean elution time is a measure of alcohol sensitivity. To measure alcohol tolerance, we subjected flies to an initial exposure to ethanol, and recorded their elution profile as described above. We allowed the flies to recover for 2 h, and then repeated the assay with the same flies. The difference in the shift of modal elution times between the second and first assa The quantitative estimate of expression of each probe set is the Signal (Sig) metric, as described in the Affymetrix Microarray Suite, Version 5.0. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. Significant probe sets were further analyzed by post hoc Tukey tests to identify which samples accounted for transcriptional differences betwee A list of probe sets differentially expressed between treatment groups with q < 0.05. Transcript levels are altered following the first exposure to ethanol, but are not sustained during the development of tolerance. (NIF Method ID 219.5)
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