GeneSet Title
Tier IV
Mouse
639 Genes
GS408917
LABEL: DEG C57BL/6J PFC CIE vs air (drinkers) FDR < 0.01_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
402 Genes
GS408918
LABEL: DEG C57BL/6J PFC CIE vs air (nondrinkers) FDR < 0.01_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
612 Genes
GS408919
LABEL: DEG C57BL/6J PFC CIE (drinkers) vs CIE (nondrinkers) FDR < 0.01_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
222 Genes
GS408920
LABEL: DEG C57BL/6J PFC CIE (drinkers) vs air (nondrinkers) FDR < 0.01_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
1538 Genes
GS408921
LABEL: DEG C57BL/6J PFC CIE (nondrinkers) vs air (drinkers) FDR < 0.01_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
407 Genes
GS408922
LABEL: DEG C57BL/6J PFC air (nondrinkers) vs air (drinkers) FDR < 0.01_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier V
Mouse
124 Genes
GS408923
LABEL: DEG C57BL/6J PFC CIE vs air (nondrinkers) FDR < 0.01 (gene symbol)_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
100 Genes
GS408924
LABEL: DEG C57BL/6J PFC CIE vs air (nondrinkers) FDR < 0.01 (gene symbol)_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
148 Genes
GS408925
LABEL: DEG C57BL/6J PFC CIE (drinkers) vs CIE (nondrinkers) FDR < 0.01 (gene symbol)_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
80 Genes
GS408926
LABEL: DEG C57BL/6J PFC CIE (drinkers) vs air (nondrinkers) FDR < 0.01_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
178 Genes
GS408927
LABEL: DEG C57BL/6J PFC CIE (nondrinkers) vs air (drinkers) FDR < 0.01 (gene symbol)_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
135 Genes
GS408928
LABEL: DEG C57BL/6J PFC air (nondrinkers) vs air (drinkers) FDR < 0.01 (gene symbol)_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
77 Genes
GS408929
LABEL: DEG C57BL/6J NAc CIE vs air (drinkers) FDR < 0.01_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
6 Genes
GS408930
LABEL: DEG C57BL/6J NAc CIE (drinkers) vs CIE (nondrinkers) FDR < 0.01_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
757 Genes
GS408931
LABEL: DEG C57BL/6J NAc CIE (drinkers) vs air (nondrinkers) FDR < 0.01_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier V
Mouse
875 Genes
GS408932
LABEL: DEG C57BL/6J NAc CIE (drinkers) vs air (nondrinkers) FDR < 0.01_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
1 Genes
GS408933
LABEL: DEG C57BL/6J NAc CIE (nondrinkers) vs air (drinkers) FDR < 0.01_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
33 Genes
GS408934
LABEL: DEG C57BL/6J NAc CIE vs air (drinkers) FDR < 0.01 (gene symbol)_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
3 Genes
GS408935
LABEL: DEG C57BL/6J NAc CIE (drinkers) vs CIE (nondrinkers) FDR < 0.01 (gene symbol)_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
286 Genes
GS408936
LABEL: DEG C57BL/6J NAc CIE (drinkers) vs air (nondrinkers) FDR < 0.01 (gene symbol)_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
399 Genes
GS408937
LABEL: DEG C57BL/6J HPC CIE vs air (drinkers) FDR < 0.01_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
32 Genes
GS408938
LABEL: DEG C57BL/6J HPC CIE (drinkers) vs CIE (nondrinkers) FDR < 0.01_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
1068 Genes
GS408939
LABEL: DEG C57BL/6J HPC CIE (drinkers) vs air (nondrinkers) FDR < 0.01_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
88 Genes
GS408940
LABEL: DEG C57BL/6J HPC CIE vs air (drinkers) FDR < 0.01 (gene symbol)_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier V
Mouse
16 Genes
GS408941
LABEL: DEG C57BL/6J HPC CIE (drinkers) vs CIE (nondrinkers) FDR < 0.01 (gene symbol)_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
7 Genes
GS408942
LABEL: DEG C57BL/6J HPC CIE (drinkers) vs CIE (nondrinkers) FDR < 0.01 (gene symbol)_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
306 Genes
GS408943
LABEL: DEG C57BL/6J HPC CIE (drinkers) vs air (nondrinkers) FDR < 0.01 (gene symbol)_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
51 Genes
GS408944
LABEL: DEG C57BL/6J CeA CIE vs air (drinkers) FDR < 0.01_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
468 Genes
GS408945
LABEL: DEG C57BL/6J CeA CIE (drinkers) vs CIE (nondrinkers) FDR < 0.01_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
502 Genes
GS408946
LABEL: DEG C57BL/6J CeA CIE (drinkers) vs air (nondrinkers) FDR < 0.01_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
20 Genes
GS408947
LABEL: DEG C57BL/6J CeA CIE vs air (drinkers) FDR < 0.01 (gene symbol)_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
143 Genes
GS408948
LABEL: DEG C57BL/6J CeA CIE (drinkers) vs CIE (nondrinkers) FDR < 0.01 (gene symbol)_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
192 Genes
GS408949
LABEL: DEG C57BL/6J CeA CIE (drinkers) vs air (nondrinkers) FDR < 0.01 (gene symbol)_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
307 Genes
GS408950
LABEL: DEG C57BL/6J BNST CIE vs air (drinkers) FDR < 0.01_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
141 Genes
GS408951
LABEL: DEG C57BL/6J BNST CIE (drinkers) vs CIE (nondrinkers) FDR < 0.01_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
377 Genes
GS408952
LABEL: DEG C57BL/6J BNST CIE (drinkers) vs air (nondrinkers) FDR < 0.01_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
76 Genes
GS408953
LABEL: DEG C57BL/6J BNST CIE vs air (drinkers) FDR < 0.01 (gene symbol)_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
30 Genes
GS408954
LABEL: DEG C57BL/6J BNST CIE (drinkers) vs CIE (nondrinkers) FDR < 0.01 (gene symbol)_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
Tier IV
Mouse
111 Genes
GS408955
LABEL: DEG C57BL/6J BNST CIE (drinkers) vs air (nondrinkers) FDR < 0.01 (gene symbol)_qvalueDESCRIPTION: Adult male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) at 10 weeks of age. Mice were kept under a 12-hour light/dark cycle and given free access to water and standard rodent chow (Harland, Teklad, Madison, WI). Studies were designed to determine genomic responses and interactions between two different ethanol exposure models: intermittent cycles of ethanol vapor exposure in inhalation chambers (CIE), and oral consumption of 15% (v/v) ethanol in a limited access (2 h/session) paradigm. Mice were divided into 4 treatment groups (n = 12/group): the “CIE Drinking” group received inhaled ethanol in the vapor chambers followed by 2-bottle choice ethanol drinking in between vapor exposure cycles; the “Air Drinking” group received only air in the vapor chambers, but had 2-bottle choice ethanol drinking between CIE cycles; the “CIE NonDrinking” group received inhaled ethanol in the vapor chambers but only water access in between CIE cycles; and the “Air NonDrinking” group remained ethanol naïve with air exposure in vapor chambers and only water consumption between CIE cycles. Following a 2-week acclimation period, mice in the CIE Drinking and Air Drinking groups underwent 6-weeks of 2-bottle choice drinking to establish baseline drinking levels. The 2-hr limited access session to ethanol (15 v/v) vs. water started 30 min before lights off. Ethanol and water intake for each individual mouse was measured daily. Following 6-weeks of baseline drinking, mice were placed in Plexiglass inhalation chambers (60x36x60 cm) 16 hours/day for 4 days. Ethanol was volatilized with an air stone submerged in 95% ethanol. Vapor chamber ethanol concentrations were monitored daily and air flow was adjusted to ethanol concentrations within 10–13 mg/l air. This ethanol vapor concentration has been shown to yield stable blood ethanol concentrations (175–225 mg/dL) in C57BL/6J mice [25]. Before each vapor chamber session, intoxication was initiated in the CIE group by administration of 1.6 g/kg ethanol and 1 mmol/kg pyrazole intraperitoneally (i.p.) at a volume of 0.02 ml/g body weight. Pyrazole is an alcohol dehydrogenase inhibitor used to stabilize blood ethanol concentrations. All mice received the same number and timing of pyrazole injections prior to final removal from the inhalation chambers with control mice receiving saline and pyrazole (i.p.), also at a volume of 0.02 ml/g body weight, prior to being placed into control vapor chambers. Control vapor chambers delivered only air without ethanol vapor. After 4 days in the inhalation chambers, mice underwent a 72-hour period of total abstinence from ethanol. Following the abstinence period, mice in the CIE Drinking and Air Drinking groups were given 2-bottle choice drinking for 2 hours per day for 5 days. A total of 4 cycles of CIE-abstinence-drinking were performed. After the end of the 4th cycle mice were sacrificed on the 5th drinking day before receiving ethanol/water access on that day at the time they received 2-bottle choice drinking all previous drinking days (Fig 1). Affymetrix GeneChip® Mouse Genome 430, type 2 arrays were analyzed with The R Project for Statistical Computing (http://www.r-project.org/). Statistical analysis to identify significantly regulated genes was performed using the limma package for R. False discovery rates equal to or less than 0.01 were considered significant.
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