GeneSet Title
Tier III
Human
675 Genes
GS1229
LABEL: HumanDiffGenExpHeroinDESCRIPTION: Gene expression changes in the post-mortem nucleus accumbens of chronic heroin abusers. Overall, little overlap in gene expression profiles was seen between the two drug-abusing cohorts: out of the approximately 39,000 transcripts investigated, the abundance of only 25 was significantly changed in both cocaine and heroin abusers, with nearly one-half of these being altered in opposite directions. 1050 Transcripts had different in abundance between the majority of heroin subjects and their matched controls.
Tier III
Human
7 Genes
GS1230
LABEL: DownRegHeroinCocaineDESCRIPTION: From the initial set of differentially expressed genes in post mortum nucleus accumbens of chronic heroin and cocaine abusers, this set contains genes downregulated in both cohorts.
Tier III
Human
5 Genes
GS1231
LABEL: UpRegHeroinCocaineDESCRIPTION: From the initial set of differentially expressed genes in post mortum nucleus accumbens of chronic heroin and cocaine abusers, this set contains genes upregulated in both cohorts.
Tier III
Human
6 Genes
GS1232
LABEL: UpRegCocaineDwnRegHeroinDESCRIPTION: From the initial set of differentially expressed genes in post mortum nucleus accumbens of chronic heroin and cocaine abusers, this set contains genes upregulated in cocaine abusers but downregulated in heroin abusers.
Tier III
Human
3 Genes
GS1234
LABEL: UpRegHeroinDwnRegCocaineDESCRIPTION: From the initial set of differentially expressed genes in post mortum nucleus accumbens of chronic heroin and cocaine abusers, this set contains genes upregulated in cocaine abusers but downregulated in heroin abusers.
Tier I
Human
DRG
21 Genes
GS86571
LABEL: Chronic heroin - Heroin vs. ControlDESCRIPTION: Chronic heroin - Heroin vs. Control DNA microarray Change in gene expression Heroin users (n = 7) exhibited a positive blood toxicology for heroin and / or its metabolites (e.g., 6-monoacetyl morphine, morphine, morphine glucuronide). Control subjects (n = 7) were matched pairwise with heroin users for gender, race, age, and brain pH. Of the 14 subjects, one control and two heroin subjects tested positive for moderate levels of alcohol (< or = to 0.11 g / dl), but did not exhibit common signs of chronic alcohol abuse. All heroin and control subjects tested negative for a Affymetrix Microarray Suite 5.0 software package, Wilcoxon signed rank test. A post hoc group-wise analysis of all genes identified on the microarray as residing within the category of synaptic transmission was performed with the non-parametric Mann-Whitney U-test (p < or = to 0.05). Transcripts increased or decreased in the majority (> or = to 4 of 7) of pairs were considered differentially expressed. (NIF Table ID 145 [115])
Tier I
Human
DRG
21 Genes
GS86937
LABEL: Chronic cocaine - Cocaine vs. ControlDESCRIPTION: Chronic cocaine - Cocaine vs. Control DNA microarray Change in gene expression Cocaine users (n = 10) exhibited a positive toxicology for cocaine and / or its metabolites. Control subjects (n = 10) were matched pair-wise with cocaine users for manner of death, age, gender, and race. Of the 20 subjects, two cocaine and two control subjects tested positive for subintoxicating doses of alcohol (g / dL < or = to 0.07) but did not exhibit common signs of chronic alcohol abuse, and alcohol did not have predictive value in hierarchical clustering of subjects based on gene express Affymetrix Microarray Suite 5.0 software package. Subjects were analyzed in pairs, comparing each cocaine sample with its matched control. Significant differences between subject pairs were calculated using the Wilcoxon signed rank test (p < or = to 0.05). Post-hoc examination confirmed the statistical significance of differential expression for the majority of transcripts (Mann-Whitney U-test, p < or = to 0.05; data not shown). Transcripts increased or decreased in the majority (> or = to 6 of 10) of pairs, representing only 0.2% of the total transcripts, were considered differentially expressed. Transcripts meeting this criterion were examined posthoc for statistical significance using Mann?Whitney U-tests (p < or = to 0.05). (NIF Table ID 145 [114])
Tier I
Human
DRG
867 Genes
GS86977
LABEL: Chronic heroin - Heroin vs. ControlDESCRIPTION: Chronic heroin - Heroin vs. Control DNA microarray Change in gene expression Heroin users (n = 7) exhibited a positive blood toxicology for heroin and / or its metabolites (e.g., 6-monoacetyl morphine, morphine, morphine glucuronide). Control subjects (n = 7) were matched pairwise with heroin users for gender, race, age, and brain pH. Of the 14 subjects, one control and two heroin subjects tested positive for moderate levels of alcohol (< or = to 0.11 g / dl), but did not exhibit common signs of chronic alcohol abuse. All heroin and control subjects tested negative for a Affymetrix Microarray Suite 5.0 software package, Wilcoxon signed rank test. A post hoc group-wise analysis of all genes identified on the microarray as residing within the category of synaptic transmission was performed with the non-parametric Mann-Whitney U-test (p< or = to 0.05). Transcripts increased or decreased in the majority (> or = to 4 of 7) of pairs were considered differentially expressed. (NIF Table ID 146 [116])
Tier I
Human
DRG
16 Genes
GS87331
LABEL: Chronic cocaine - Cocaine vs. ControlDESCRIPTION: Chronic cocaine - Cocaine vs. Control DNA microarray Change in gene expression Cocaine users (n = 10) exhibited a positive toxicology for cocaine and / or its metabolites. Control subjects (n = 10) were matched pair-wise with cocaine users for manner of death, age, gender, and race. Of the 20 subjects, two cocaine and two control subjects tested positive for subintoxicating doses of alcohol (g / dL < or = to 0.07) but did not exhibit common signs of chronic alcohol abuse, and alcohol did not have predictive value in hierarchical clustering of subjects based on gene express Affymetrix Microarray Suite 5.0 software package. Subjects were analyzed in pairs, comparing each cocaine sample with its matched control. Significant differences between subject pairs were calculated using the Wilcoxon signed rank test (p < or = to 0.05). Post-hoc examination confirmed the statistical significance of differential expression for the majority of transcripts (Mann-Whitney U-test, p < or = to 0.05; data not shown). Transcripts increased or decreased in the majority (> or = to 6 of 10) of pairs, representing only 0.2% of the total transcripts, were considered differentially expressed. Transcripts meeting this criterion were examined posthoc for statistical significance using Mann?Whitney U-tests (p < or = to 0.05). (NIF Method ID 114)
Tier I
Human
DRG
16 Genes
GS87332
LABEL: Chronic heroin - Heroin vs. ControlDESCRIPTION: Chronic heroin - Heroin vs. Control DNA microarray Change in gene expression Heroin users (n = 7) exhibited a positive blood toxicology for heroin and / or its metabolites (e.g., 6-monoacetyl morphine, morphine, morphine glucuronide). Control subjects (n = 7) were matched pairwise with heroin users for gender, race, age, and brain pH. Of the 14 subjects, one control and two heroin subjects tested positive for moderate levels of alcohol (< or = to 0.11 g / dl), but did not exhibit common signs of chronic alcohol abuse. All heroin and control subjects tested negative for a Affymetrix Microarray Suite 5.0 software package, Wilcoxon signed rank test. A post hoc group-wise analysis of all genes identified on the microarray as residing within the category of synaptic transmission was performed with the non-parametric Mann-Whitney U-test (p < or = to 0.05). Transcripts increased or decreased in the majority (> or = to 4 of 7) of pairs were considered differentially expressed. (NIF Method ID 115)
Tier I
Human
DRG
911 Genes
GS87333
LABEL: Chronic heroin - Heroin vs. ControlDESCRIPTION: Chronic heroin - Heroin vs. Control DNA microarray Change in gene expression Heroin users (n = 7) exhibited a positive blood toxicology for heroin and / or its metabolites (e.g., 6-monoacetyl morphine, morphine, morphine glucuronide). Control subjects (n = 7) were matched pairwise with heroin users for gender, race, age, and brain pH. Of the 14 subjects, one control and two heroin subjects tested positive for moderate levels of alcohol (< or = to 0.11 g / dl), but did not exhibit common signs of chronic alcohol abuse. All heroin and control subjects tested negative for a Affymetrix Microarray Suite 5.0 software package, Wilcoxon signed rank test. A post hoc group-wise analysis of all genes identified on the microarray as residing within the category of synaptic transmission was performed with the non-parametric Mann-Whitney U-test (p< or = to 0.05). Transcripts increased or decreased in the majority (> or = to 4 of 7) of pairs were considered differentially expressed. (NIF Method ID 116)
Tier V
Human
1 Genes
GS219163
LABEL: Heroin upregulatedDESCRIPTION: Set of genes from Alberts et al via DRG that are upregulated in response to heroin
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