GeneSet Information

Tier III GS75617 • General Inflammatory Bowel Disease-Associated Sequences in PBMCs - Ulcerative Colitis

DESCRIPTION:

Before the expression profiling portion of the study, the cellular compositions of the purified PBMC pellets from patients in all three groups (healthy patients, patients with CD, and patients with UC) were measured before RNA isolation.

LABEL:

PBMCs - UC

SCORE TYPE:

P-Value

DATE ADDED:

2010-06-22

DATE UPDATED:

2020-05-06

SPECIES:

AUTHORS:

Burczynski ME, Peterson RL, Twine NC, Zuberek KA, Brodeur BJ, Casciotti L, Maganti V, Reddy PS, Strahs A, Immermann F, Spinelli W, Schwertschlag U, Slager AM, Cotreau MM, Dorner AJ

TITLE:

Molecular classification of Crohn\'s disease and ulcerative colitis patients using transcriptional profiles in peripheral blood mononuclear cells.

JOURNAL:

The Journal of molecular diagnostics : JMD Feb 2006, Vol 8, pp. 51-61

ABSTRACT:

Ulcerative colitis (UC) and Crohn\'s disease (CD) are common inflammatory bowel diseases producing intestinal inflammation and tissue damage. Although emerging evidence suggests these diseases are distinct, approximately 10% of patients remain classified as indeterminate inflammatory bowel disease even after invasive colonoscopy intended for diagnosis. A molecular diagnostic assay using a clinically accessible tissue would greatly assist in the classification of these diseases. In the present study we assessed transcriptional profiles in peripheral blood mononuclear cells from 42 healthy individuals, 59 CD patients, and 26 UC patients by hybridization to microarrays interrogating more than 22,000 sequences. Supervised analysis identified a set of 12 genes that distinguished UC and CD patient samples with high accuracy. The alterations in transcript levels observed by microarray were verified by real-time polymerase chain reaction. The results suggest that a peripheral blood mononuclear cell-based gene expression signature can provide a molecular biomarker that can complement the standard diagnosis of UC and CD. PUBMED: 16436634
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Annotation Information

No sequence read archive data associated with this GeneSet.


RNA (D012313)
Humans (D006801)
Molecular Diagnostic Techniques (D025202)
Polymerase Chain Reaction (D016133)
Patients (D010361)
Gene Expression Profiling (D020869)
Case-Control Studies (D016022)
Leukocytes, Mononuclear (D007963)
Adult (D000328)
Classification (D002965)
Reverse Transcriptase Polymerase Chain Reaction (D020133)
Tissues (D014024)
Inflammation (D007249)
Colonoscopy (D003113)
Cells (D002477)
Blood (D001769)
Diagnosis (D003933)
Colitis, Ulcerative (D003093)
Colitis (D003092)
Complement System Proteins (D003165)
Inflammatory Bowel Diseases (D015212)
Pathology, Molecular (D057089)
Crohn Disease (D003424)
Set (Psychology) (D012718)
blood (MA:0000059)
bowel (MA:0001524)
colitis (MP:0002816)
intestinal inflammation (MP:0001858)
abnormal inflammatory response (MP:0001845)
gene expression (GO:0010467)

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Supported by NIH R01 AA18776 jointly funded by NIAAA and NIDA, and JAX Center for Precision Genetics NIH U54 OD 020351