Differential gene expression in nucleus accumbens somatostatin interneurons_cocaine_mice_pvalue
Description:
To characterize transcriptional alterations that cocaine induces in these cells, we perform cell type-specific RNA-sequencing on FACS-isolated nuclei of somatostatin interneurons and identified 1100 DETs enriched for processes related to neural plasticity. To profile the entire (non poly-A selected) transcriptome of NAc somatostatin interneurons, we generated a transgenic reporter line (SST-TLG498 mice) to label the nuclei of these cells with a modified form of EGFP that is retained in the nuclear membrane (EGFP-F)22, enabling their isolation from NAc dissections using FACS. We succeeded in FACS-isolating nuclei suitable for RNA-sequencing from individual SST-TLG498 mice. We proceeded with differential expression analysis of the RNA-sequencing data to identify differentially expressed transcripts (DETs) in NAc somatostatin interneurons in response to repeated cocaine exposure: 778 transcripts were upregulated by cocaine and 322 were downregulated.
Authors:
Efrain A Ribeiro, Marine Salery, Joseph R Scarpa, Erin S Calipari, Peter J Hamilton, Stacy M Ku, Hope Kronman, Immanuel Purushothaman, Barbara Juarez, Mitra Heshmati, Marie Doyle, Casey Lardner, Dominicka Burek, Ana Strat, Stephen Pirpinias, Ezekiell Mouzon, Ming-Hu Han, Rachael L Neve, Rosemary C Bagot, Andrew Kasarskis, Ja Wook Koo, Eric J Nestler
QTL associated with vertebral trabecular bone trait 8. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (85791117)
Authors:
Bouxsein ML, Uchiyama T, Rosen CJ, Shultz KL, Donahue LR, Turner CH, Sen S, Churchill GA, Mller R, Beamer WG
Alcohol preference QTL 1 spans 26931283-76931283 (NCBI Build 37) on Chr9. This interval was obtained by using an interval width of 25 Mbp around the peak marker (Build 37, MGI, http://informatics.jax.org). Phenotypically extreme HAP1/LAP1 animals (n=96) and HAP2/LAP2 animals (n=48) were screened for microsatellite markers in chromosomal regions previously reported to influence alcohol preference phenotypes. Linkage to alcohol preference, Alpq1, mapped to chromosome 9 near D9Mit4 (29 cM) in the HAP1/LAP1 set and near D9Mit90 (9 cM) in the HAP2/LAP2 set. The Alpq1 QTL interval is broad and may contain more than 1 underlying gene. Drd2 at 28 cM is a potential candidate for Alpq1.
Authors:
Bice PJ, Foroud T, Carr LG, Zhang L, Liu L, Grahame NJ, Lumeng L, Li TK, Belknap JK
QTL associated with bleomycin-induced pulmonary fibrosis 3. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (79938718)
QTL associated with haloperidol-induced catalepsy 1. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (86440120)
Authors:
Hitzemann R, Demarest K, Koyner J, Cipp L, Patel N, Rasmussen E, McCaughran J Jr
QTL associated with vertebral trabecular bone trait 7. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (47636700)
Authors:
Bouxsein ML, Uchiyama T, Rosen CJ, Shultz KL, Donahue LR, Turner CH, Sen S, Churchill GA, Mller R, Beamer WG
QTL associated with susceptibility to Salmonella typhimurium antigens 7. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (58210366)
Authors:
de Souza CM, Morel L, Cabrera WH, Starobinas N, Ribeiro OG, Siqueira M, Ibaez OM, De Franco M
QTL associated with taste bitterness sensitivity 11. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (76310471)
QTL associated with APP associated premature death 1. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (40462577)
QTL for high-dose ethanol actions on Chr9 at D9Mit42 (21.79 Mbp , Build 37)
Description:
high-dose ethanol actions spans 0.00 - 46.79 Mbp (NCBI Build 37) on Chr9. This interval was obtained by using an interval width of 25 Mbp around the peak marker (Build 37, MGI, http://informatics.jax.org).
Authors:
Erwin VG, Markel PD, Johnson TE, Gehle VM, Jones BC
BECs at LORR Recovery Chr# 9 rs13480854(7524005) with right flanking marker mCV23893269 (4062079) and left marker rs3663313 (123063108). This was mapped in 300 + (b6x129)F2 mice.
Ethanol Induced Ataxia Chr#9 rs3655717(65312971) with right flanking marker rs13480112(26413932) and left marker rs13480421(111761261). This was mapped in 300 + (b6x129)F2 mice.
QTL associated with pulmonary adenoma susceptibility 4. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (86133452)
QTL associated with streptozotocin induced diabetes susceptibility 2. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (86542264)
Authors:
Gonzalez C, Cuvellier S, Hue-Beauvais C, Lvi-Strauss M
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