QTL associated with methamphetamine response QTL 3. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (115323097)
Authors:
Palmer AA, Verbitsky M, Suresh R, Kamens HM, Reed CL, Li N, Burkhart-Kasch S, McKinnon CS, Belknap JK, Gilliam TC, Phillips TJ
Differential gene expression in nucleus accumbens somatostatin interneurons_cocaine_mice_pvalue
Description:
To characterize transcriptional alterations that cocaine induces in these cells, we perform cell type-specific RNA-sequencing on FACS-isolated nuclei of somatostatin interneurons and identified 1100 DETs enriched for processes related to neural plasticity. To profile the entire (non poly-A selected) transcriptome of NAc somatostatin interneurons, we generated a transgenic reporter line (SST-TLG498 mice) to label the nuclei of these cells with a modified form of EGFP that is retained in the nuclear membrane (EGFP-F)22, enabling their isolation from NAc dissections using FACS. We succeeded in FACS-isolating nuclei suitable for RNA-sequencing from individual SST-TLG498 mice. We proceeded with differential expression analysis of the RNA-sequencing data to identify differentially expressed transcripts (DETs) in NAc somatostatin interneurons in response to repeated cocaine exposure: 778 transcripts were upregulated by cocaine and 322 were downregulated.
Authors:
Efrain A Ribeiro, Marine Salery, Joseph R Scarpa, Erin S Calipari, Peter J Hamilton, Stacy M Ku, Hope Kronman, Immanuel Purushothaman, Barbara Juarez, Mitra Heshmati, Marie Doyle, Casey Lardner, Dominicka Burek, Ana Strat, Stephen Pirpinias, Ezekiell Mouzon, Ming-Hu Han, Rachael L Neve, Rosemary C Bagot, Andrew Kasarskis, Ja Wook Koo, Eric J Nestler
Differential gene expression in nucleus accumbens somatostatin interneurons_cocaine_mice_subset_pvalue0.05
Description:
To characterize transcriptional alterations that cocaine induces in these cells, we perform cell type-specific RNA-sequencing on FACS-isolated nuclei of somatostatin interneurons and identified 1100 DETs enriched for processes related to neural plasticity. To profile the entire (non poly-A selected) transcriptome of NAc somatostatin interneurons, we generated a transgenic reporter line (SST-TLG498 mice) to label the nuclei of these cells with a modified form of EGFP that is retained in the nuclear membrane (EGFP-F)22, enabling their isolation from NAc dissections using FACS. We succeeded in FACS-isolating nuclei suitable for RNA-sequencing from individual SST-TLG498 mice. We proceeded with differential expression analysis of the RNA-sequencing data to identify differentially expressed transcripts (DETs) in NAc somatostatin interneurons in response to repeated cocaine exposure: 778 transcripts were upregulated by cocaine and 322 were downregulated. This dataset only includes a subset of the full dataset, thresholded at p<0.05.
Authors:
Efrain A Ribeiro, Marine Salery, Joseph R Scarpa, Erin S Calipari, Peter J Hamilton, Stacy M Ku, Hope Kronman, Immanuel Purushothaman, Barbara Juarez, Mitra Heshmati, Marie Doyle, Casey Lardner, Dominicka Burek, Ana Strat, Stephen Pirpinias, Ezekiell Mouzon, Ming-Hu Han, Rachael L Neve, Rosemary C Bagot, Andrew Kasarskis, Ja Wook Koo, Eric J Nestler
QTL associated with femur length in high growth mice 4. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (76866991)
QTL associated with weight gain in high growth mice 7. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (96868181)
QTL associated with gonadal fat pad weight QTL 1. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (114084818)
QTL associated with prion disease incubation time 3. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (83656096)
Authors:
Lloyd SE, Uphill JB, Targonski PV, Fisher EM, Collinge J
QTL associated with systematic lupus erythematosus susceptibility 13. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (87613874)
Authors:
Morel L, Mohan C, Yu Y, Schiffenbauer J, Rudofsky UH, Tian N, Longmate JA, Wakeland EK
QTL associated with streptozotocin induced diabetes susceptibility. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (90114273)
Authors:
Babaya N, Ikegami H, Fujisawa T, Nojima K, Itoi-Babaya M, Inoue K, Ohno T, Shibata M, Ogihara T
QTL associated with "alcohol preference locus 18, male specific". This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (112374453)
QTL associated with "alcohol preference locus 20, female specific". This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (112374453)
cocaine related behavior 11 (Cocrb11) spans 0 - 27.768945 Mbp (NCBI Build 37) on Chr 11. Obtained from MGI (http://www.informatics.jax.org) by searching for QTLs containing the keyword .
QTL for differences in cocaine responsiveness on Chr11 at D11M!t2 (8.35 Mbp , Build 37)
Description:
differences in cocaine responsiveness spans 0.00 - 33.35 Mbp (NCBI Build 37) on Chr11. This interval was obtained by using an interval width of 25 Mbp around the peak marker (Build 37, MGI, http://informatics.jax.org).
QTL for cocaine related behavior on Chr11 at Glns-ps1 (18.69 Mbp , Build 37)
Description:
cocaine related behavior spans 0.00 - 43.69 Mbp (NCBI Build 37) on Chr11. This interval was obtained by using an interval width of 25 Mbp around the peak marker (Build 37, MGI, http://informatics.jax.org).
QTL for chronic alcohol withdrawal severity on Chr11 at D11Mit340 (18.69 Mbp , Build 37)
Description:
chronic alcohol withdrawal severity spans 0.00 - 43.69 Mbp (NCBI Build 37) on Chr11. This interval was obtained by using an interval width of 25 Mbp around the peak marker (Build 37, MGI, http://informatics.jax.org).
Authors:
Bergeson SE, Kyle Warren R, Crabbe JC, Metten P, Gene Erwin V, Belknap JK
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