Differential gene expression in nucleus accumbens somatostatin interneurons_cocaine_mice_pvalue
Description:
To characterize transcriptional alterations that cocaine induces in these cells, we perform cell type-specific RNA-sequencing on FACS-isolated nuclei of somatostatin interneurons and identified 1100 DETs enriched for processes related to neural plasticity. To profile the entire (non poly-A selected) transcriptome of NAc somatostatin interneurons, we generated a transgenic reporter line (SST-TLG498 mice) to label the nuclei of these cells with a modified form of EGFP that is retained in the nuclear membrane (EGFP-F)22, enabling their isolation from NAc dissections using FACS. We succeeded in FACS-isolating nuclei suitable for RNA-sequencing from individual SST-TLG498 mice. We proceeded with differential expression analysis of the RNA-sequencing data to identify differentially expressed transcripts (DETs) in NAc somatostatin interneurons in response to repeated cocaine exposure: 778 transcripts were upregulated by cocaine and 322 were downregulated.
Authors:
Efrain A Ribeiro, Marine Salery, Joseph R Scarpa, Erin S Calipari, Peter J Hamilton, Stacy M Ku, Hope Kronman, Immanuel Purushothaman, Barbara Juarez, Mitra Heshmati, Marie Doyle, Casey Lardner, Dominicka Burek, Ana Strat, Stephen Pirpinias, Ezekiell Mouzon, Ming-Hu Han, Rachael L Neve, Rosemary C Bagot, Andrew Kasarskis, Ja Wook Koo, Eric J Nestler
QTL associated with immediate cutaneous hypersensitivity QTL. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (107745604)
Authors:
Daser A, Koetz K, Btjer N, Jung M, Rschendorf F, Goltz M, Ellerbrok H, Renz H, Walter J, Paulsen M
QTL associated with "early growth rate, maternal effect 2". This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (87375892)
QTL associated with ethanol induced locomotor activity 2. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (87375892)
QTL associated with femoral cross-sectional area 1. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (88931018)
Authors:
Klein RF, Turner RJ, Skinner LD, Vartanian KA, Serang M, Carlos AS, Shea M, Belknap JK, Orwoll ES
QTL associated with modifier of Salmonella typhimurium susceptibility 2. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (75397577)
Authors:
Borrego A, Peters LC, Jensen JR, Ribeiro OG, Koury Cabrera WH, Starobinas N, Seman M, Ibaez OM, De Franco M
QTL for morphine preference on Chr6 at NA (1.89 Mbp , Build 37)
Description:
morphine preference spans 0.00 - 26.89 Mbp (NCBI Build 37) on Chr6. This interval was obtained by using an interval width of 25 Mbp around the peak marker (Build 37, MGI, http://informatics.jax.org).
QTL for METH responses for home cage activity on Chr6 at Met (13.99 Mbp , Build 37)
Description:
METH responses for home cage activity spans 0.00 - 38.99 Mbp (NCBI Build 37) on Chr6. This interval was obtained by using an interval width of 25 Mbp around the peak marker (Build 37, MGI, http://informatics.jax.org).
QTL associated with cystic fibrosis body weight 2. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (111954645)
QTL associated with obesity and body weight QTL 3. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (107745604)
QTL associated with TNF-induced lethal shock susceptibility 1. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (110909916)
Authors:
Wielockx B, Staelens J, Puimge L, Vanlaere I, Van Roy M, van Lint P, Van Roy F, Libert C
Average rotarod training latency Chr# 6 rs6239023 (94005991) with right flanking marker rs3672029(75345665) and left marker rs30316697(130188177). This was mapped in 300 + (b6x129)F2 mice.
QTL associated with collagen induced arthritis QTL 6. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (75397577)
QTL associated with gastritis type A susceptibility locus 3. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (105955399)
Authors:
Baxter AG, Jordan MA, Silveira PA, Wilson WE, Van Driel IR
QTL associated with ovarian teratoma susceptibility 1. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (96605945)
Authors:
Lee GH, Bugni JM, Obata M, Nishimori H, Ogawa K, Drinkwater NR
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