QTL for nicotine sensitivity on Chr8 at D8Mit124 (13.44 Mbp , Build 37)
Description:
nicotine sensitivity spans 0.00 - 38.44 Mbp (NCBI Build 37) on Chr8. This interval was obtained by using an interval width of 25 Mbp around the peak marker (Build 37, MGI, http://informatics.jax.org).
alcohol preference spans 0.00 - 40.29 Mbp (NCBI Build 37) on Chr8. This interval was obtained by using an interval width of 25 Mbp around the peak marker (Build 37, MGI, http://informatics.jax.org).
Authors:
Bachmanov AA, Reed DR, Li X, Li S, Beauchamp GK, Tordoff MG
QTL associated with bone response to mechanical loading 2. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (117360795)
QTL associated with bone response to mechanical loading 3. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (126594840)
Authors:
Kesavan C, Mohan S, Srivastava AK, Kapoor S, Wergedal JE, Yu H, Baylink DJ
QTL associated with wound healing/regeneration 1. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (105240821)
Authors:
Heber-Katz E, Leferovich JM, Bedelbaeva K, Gourevitch D
QTL associated with heterogeneity in eye lens protein photooxidation kinetics. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (129076217)
Authors:
Wisser KC, Schauerte JA, Burke DT, Galecki A, Chen S, Miller RA, Gafni A
QTL associated with mean platelet volume locus 2. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (105240821)
QTL associated with novelty/stress induced locomotor activation 7. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (119052549)
QTL associated with susceptibility to lung cancer 9. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (118625692)
QTL associated with tibia bone quality traits 2. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (129578831)
Authors:
Jiao Y, Chiu H, Fan Z, Jiao F, Eckstein EC, Beamer WG, Gu W
QTL associated with vertebral morphology and mechanical traits 9. This interval was obtained by using a fixed interval width of 25 Mbp around the peak marker (129076217)
Authors:
Reeves GM, McCreadie BR, Chen S, Galecki AT, Burke DT, Miller RA, Goldstein SA
Drug Naïve DO mice were tested for open field, light dark, hole board, novelty place preference before collecting the striatum. RNA-Seq data was analyzed with WGCNA using a soft thresholding power of 3 selected using the WGCNA scale-free topology R2 threshold of 0.9, signed network with a minimum module size of 30, correlation type is bicor, used numeric labels.
Alcohol transcriptome changes in mice microglia total homogenate p-value
Description:
Microglia are fundamentally important immune cells within the central nervous system (CNS) that respond to environmental challenges to maintain normal physiological processes. Alterations in steady-state cellular function and over-activation of microglia can facilitate the initiation and progression of neuropathological conditions such as Alzheimer’s disease, Multiple Sclerosis, and Major Depressive Disorder. Alcohol consumption disrupts signaling pathways including both innate and adaptive immune responses that are necessary for CNS homeostasis. Coordinate expression of these genes is not ascertained from an admixture of CNS cell-types, underscoring the importance of examining isolated cellular populations to reveal systematic gene expression changes arising from mature microglia. Unbiased RNA-Seq profiling was used to identify gene expression changes in isolated prefrontal cortical microglia in response to recurring bouts of voluntary alcohol drinking behavior. The voluntary ethanol paradigm utilizes long-term consumption ethanol that results in escalated alcohol intake and altered cortical plasticity that is seen in humans. Gene coexpression analysis identified a coordinately regulated group of genes, unique to microglia, that collectively are associated with alcohol consumption. Genes within this group are involved in toll-like receptor signaling and transforming growth factor beta signaling. Network connectivity of this group identified Siglech as a putative hub gene and highlighted the potential importance of proteases in the microglial response to chronic ethanol. In conclusion, we identified a distinctive microglial gene expression signature for neuroimmune responses related to alcohol consumption that provides valuable insight into microglia-specific changes underlying the development of substance abuse, and possibly other CNS disorders.
Authors:
Gizelle M McCarthy, Sean P Farris, Yuri A Blednov, R Adron Harris, R Dayne Mayfield
The current study used two inbred mouse strains, C57BL/6 J and A/J, to investigate the genetics of behavioral responses to fentanyl. Mice were tested for conditioned place preference and fentanyl-induced locomotor activity. C57BL/6J mice formed a conditioned place preference to fentanyl injections and fentanyl increased their activity. Neither effect was noted in A/J mice. We conducted RNA-sequencing on the nucleus accumbens of mice used for fentanyl-induced locomotor activity. Surprisingly, we noted few differentially expressed genes using treatment as the main factor. However many genes differed between strains.
Authors:
Samuel J Harp, Mariangela Martini, Will Rosenow, Larry D Mesner, Hugh Johnson, Charles R Farber, Emilie F Rissman
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