Publication Details

Identification of an acute ethanol response quantitative trait locus on mouse chromosome 2.

Authors:Demarest K, McCaughran J Jr, Mahjubi E, Cipp L, Hitzemann R
Title:Identification of an acute ethanol response quantitative trait locus on mouse chromosome 2.
Journal:The Journal of neuroscience : the official journal of the Society for Neuroscience Jan 1999 , Vol 19 , pp. 549-61
Abstract:A two-stage strategy was used to identify and confirm quantitative trait loci (QTLs) associated with the changes in locomotor activity induced by a 1.5 gm/kg ethanol challenge. For stage 1, putative QTLs were identified by analysis of the strain means for 25 strains of the BXD recombinant inbred (RI) series (males only). QTLs were identified on chromosomes 1, 2, 4, and 6. The activity response to chlordiazepoxide generated similar QTLs on chromosomes 2 and 6. None of the QTLs were similar to those generated from analysis of the saline response data. For stage 2, 900 male C57BL/6J (B6) x DBA/2J (D2) F2 intercross animals were phenotyped for ethanol response, and the phenotypic extremes (those animals > and <1 SD from the mean) were identified. These extremes differed by >10,000 cm/15 min in their response to ethanol. The extreme progeny were used for a genome-wide scan both to confirm the putative RI-generated QTLs and to detect new QTLs. The F2 analysis generated no new QTLs with logarithm of the likelihood for linkage (LOD) scores >3. For RI-generated QTLs, only the QTL on chromosome 2 was confirmed (LOD = 5.3). The position of the peak LOD was estimated to be 47 cM with a 20 cM 1 LOD support interval; this QTL accounted for 6% of the phenotypic variance. The 1 LOD support interval overlaps with QTLs previously identified for alcohol preference and acute ethanol withdrawal (;; ).   PUBMED: 9880575
Ontological Annotations:
  • D040641: Quantitative Trait Loci (Publication, NCBO Annotator)
  • D002875: Chromosomes (Publication, NCBO Annotator)
  • D007062: Identification (Psychology) (Publication, NCBO Annotator)
  • D051379: Mice (Publication, NCBO Annotator)
  • D000431: Ethanol (Publication, NCBO Annotator)
  • GO:0045471: response to ethanol (Publication, NCBO Annotator)
  • MP:0001987: alcohol preference (Publication, NCBO Annotator)
  • D009043: Motor Activity (Publication, NCBO Annotator)
  • D002707: Chlordiazepoxide (Publication, NCBO Annotator)
  • GO:0005694: chromosome (Publication, NCBO Annotator)
  • D002889: Chromosomes, Human, Pair 2 (Publication, NCBO Annotator)
  • D000818: Animals (Publication, NCBO Annotator)

2 GeneSets from this Publication:

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Expand Tier II Mouse 527 Genes GS84130: ethanol response acute (Published QTL, Chr 2)
Expand Tier II Mouse 540 Genes GS135825: ethanol response acute (Etohr, Published QTL Chr 2)