Publication Details

Mapping murine loci for seizure response to kainic acid.

Authors:Ferraro TN, Golden GT, Smith GG, Schork NJ, St Jean P, Ballas C, Choi H, Berrettini WH
Title:Mapping murine loci for seizure response to kainic acid.
Journal:Mammalian genome : official journal of the International Mammalian Genome Society Mar 1997 , Vol 8 , pp. 200-8
Abstract:Mature DBA/2J (D2) mice are very sensitive to seizures induced by various chemical and physical stimuli, whereas C57BL/6J (B6) mice are relatively seizure resistant. We have conducted a genome-wide search for quantitative trait loci (QTLs) influencing the differential sensitivity of these strains to kainic acid (KA)-induced seizures by studying an F2 intercross population. Parental, F1, and F2 mice (8-10 weeks of age) were injected subcutaneously with 25 mg/kg of KA and observed for 3 h. Latencies to focal and generalized seizures and status epilepticus were recorded and used to calculate an overall seizure score. Results of seizure testing indicated that the difference in susceptibility to KA-induced seizures between D2 and B6 mice is a polygenic phenomenon with at least 65% of the variance due to genetic factors. First-pass genome screening (10-cM marker intervals) in F2 progeny (n = 257) documented a QTL of moderate effect on Chromosome (Chr) 1 with a peak LOD score of 5.5 (17% of genetic variance explained) localized between D1Mit30 and D1Mit16. Provisional QTLs of small effect were detected on Chr 11 (D11Mit224-D11Mit14), 15 (D15Mit6-D15Mit46) and 18 (D18Mit9-D18Mit144). Multiple locus models generally confirmed the Mapmaker/QTL results and also provided evidence for another QTL on Chr 4 (D4Mit9). Multilocus analysis of seizure severity suggested that additional loci on Chrs 5 (D5Mit11), 7 (D7Mit66), and 15 (D15Nds2) might also contribute to KA-induced seizure response. Overall, our results document a complex genetic determinism for KA-induced seizures in these mouse strains with contributions from as many as eight QTLs.   PUBMED: 9069121
Ontological Annotations:
  • D015211: Zellweger Syndrome (Publication, NCBO Annotator)
  • D040641: Quantitative Trait Loci (Publication, NCBO Annotator)
  • D033141: Genetic Determinism (Publication, NCBO Annotator)
  • D002875: Chromosomes (Publication, NCBO Annotator)
  • D016424: Overall (Publication, NCBO Annotator)
  • D051379: Mice (Publication, NCBO Annotator)
  • D008403: Mass Screening (Publication, NCBO Annotator)
  • D012640: Seizures (Publication, NCBO Annotator)
  • D007608: Kainic Acid (Publication, NCBO Annotator)
  • D008126: Lod Score (Publication, NCBO Annotator)
  • GO:0005694: chromosome (Publication, NCBO Annotator)
  • D013226: Status Epilepticus (Publication, NCBO Annotator)
  • MP:0002064: seizures (Publication, NCBO Annotator)

4 GeneSets from this Publication:

Select gene sets below using the check boxes, and then add them to a project using the pull down below. You can also create a project using the pull down. To view a set, click on the GeneSetID:GeneSet Description shown in green below.
Expand Tier I Mouse 459 Genes GS129191: seizure susceptibility 2 (Szs2 Published QTL Chr 4)
Expand Tier I Mouse 801 Genes GS136780: seizure susceptibility 3 (Szs3, Published QTL Chr 11)
Expand Tier I Mouse 341 Genes GS136783: seizure severity 1 (Szv1, Published QTL Chr 5)
Expand Tier I Mouse 874 Genes GS136784: seizure severity 2 (Szv2, Published QTL Chr 7)