Publication Details

Differential gene expression in the nucleus accumbens with ethanol self-administration in inbred alcohol-preferring rats.

Authors:Rodd ZA, Kimpel MW, Edenberg HJ, Bell RL, Strother WN, McClintick JN, Carr LG, Liang T, McBride WJ
Title:Differential gene expression in the nucleus accumbens with ethanol self-administration in inbred alcohol-preferring rats.
Journal:Pharmacology, biochemistry, and behavior Jun 2008 , Vol 89 , pp. 481-98
Abstract:The current study examined the effects of operant ethanol (EtOH) self-administration on gene expression kin the nucleus accumbens (ACB) and amygdala (AMYG) of inbred alcohol-preferring (iP) rats. Rats self-trained on a standard two-lever operant paradigm to administer either water-water, EtOH (15% v/v)-water, or saccharin (SAC; 0.0125% g/v)-water. Animals were killed 24 h after the last operant session, and the ACB and AMYG dissected; RNA was extracted and purified for microarray analysis. For the ACB, there were 513 significant differences at the p<0.01 level in named genes: 55 between SAC and water; 215 between EtOH and water, and 243 between EtOH and SAC. In the case of the AMYG (p<0.01), there were 48 between SAC and water, 23 between EtOH and water, and 63 between EtOH and SAC group. Gene Ontology (GO) analysis indicated that differences in the ACB between the EtOH and SAC groups could be grouped into 15 significant (p<0.05) categories, which included major categories such as synaptic transmission, cell and ion homeostasis, and neurogenesis, whereas differences between the EtOH and water groups had only 4 categories, which also included homeostasis and synaptic transmission. Several genes were in common between the EtOH and both the SAC and water groups in the synaptic transmission (e.g., Cav2, Nrxn3, Gabrb2, Gad1, Homer1) and homeostasis (S100b, Prkca, Ftl1) categories. Overall, the results suggest that changes in gene expression in the ACB of iP rats are associated with the reinforcing effects of EtOH.   PUBMED: 18405950
Ontological Annotations:
  • D009714: Nucleus Accumbens (Publication, NCBO Annotator; PubMed MeSH Annotations)
  • MA:0000887: amygdala (Publication, NCBO Annotator)
  • D006706: Homeostasis (Publication, NCBO Annotator)
  • D008959: Models, Neurological (PubMed MeSH Annotations)
  • D016424: Overall (Publication, NCBO Annotator)
  • D020411: Oligonucleotide Array Sequence Analysis (PubMed MeSH Annotations)
  • D003216: Conditioning, Operant (PubMed MeSH Annotations)
  • D012439: Saccharin (PubMed MeSH Annotations; Publication, NCBO Annotator)
  • GO:0010467: gene expression (Publication, NCBO Annotator)
  • D051381: Rats (Publication, NCBO Annotator; PubMed MeSH Annotations)
  • D055495: Neurogenesis (Publication, NCBO Annotator)
  • D000818: Animals (PubMed MeSH Annotations; Publication, NCBO Annotator)
  • D000679: Amygdala (Publication, NCBO Annotator; PubMed MeSH Annotations)
  • GO:0022008: neurogenesis (Publication, NCBO Annotator)
  • D009435: Synaptic Transmission (Publication, NCBO Annotator)
  • D000431: Ethanol (PubMed MeSH Annotations; Publication, NCBO Annotator)
  • GO:0050801: ion homeostasis (Publication, NCBO Annotator)
  • D002477: Cells (Publication, NCBO Annotator)
  • GO:0007268: synaptic transmission (Publication, NCBO Annotator)
  • GO:0005623: cell (Publication, NCBO Annotator)
  • D020133: Reverse Transcriptase Polymerase Chain Reaction (PubMed MeSH Annotations)
  • D014867: Water (Publication, NCBO Annotator)
  • GO:0005634: nucleus (Publication, NCBO Annotator)
  • D008960: Models, Psychological (PubMed MeSH Annotations)
  • D012313: RNA (Publication, NCBO Annotator)
  • D011919: Rats, Inbred Strains (PubMed MeSH Annotations)
  • D046228: Microarray Analysis (Publication, NCBO Annotator)
  • D012646: Self Administration (PubMed MeSH Annotations)
  • D000437: Alcoholism (PubMed MeSH Annotations)

3 GeneSets from this Publication:


Select gene sets below using the check boxes, and then add them to a project using the pull down below. You can also create a project using the pull down. To view a set, click on the GeneSetID:GeneSet Description shown in green below.
Expand Tier III Rat 179 Genes GS14929: Ethanol-dependence genes in the nucleus accumbens (NA) of inbred alcohol-preferring
Expand Tier III Rat 110 Genes GS14930: Ethanol-dependence genes in the amygdala (Amyg) of inbred alcohol-preferring rats
Expand Tier III Rat 190 Genes GS75567: Genes that were significantly different in the nucleus accumbens of iP rats between the ethanol and water groups