Publication Details

Transcriptional profiling of the rat frontal cortex following administration of the mGlu5 receptor antagonists MPEP and MTEP.

Authors:Gass JT, Olive MF
Title:Transcriptional profiling of the rat frontal cortex following administration of the mGlu5 receptor antagonists MPEP and MTEP.
Journal:European journal of pharmacology Apr 2008 , Vol 584 , pp. 253-62
Abstract:The development of selective type 5 metabotropic glutamate receptor (mGlu5) antagonists, such as 2-methyl-6-(phenylethynyl)-pyridine (MPEP) and 3-[(2-methyl-1,3-thiazol-4-yl)ethynyl]-pyridine (MTEP), has revealed an important role for these receptors in various disorders of the nervous system including depression, anxiety, epilepsy, Parkinson's disease, drug addiction, and alcoholism. In this study, we used microarray technology to examine changes in gene expression induced by repeated administration of the mGlu5 antagonists MPEP and MTEP. Male Wistar rats (n=5 per treatment group) were administered MPEP (10 mg/kg), MTEP (10 mg/kg) or vehicle intraperitoneally twice daily for 5 days. Approximately 30 min following the final drug administration, rats were sacrificed and frontal cortices were then dissected and examined for changes in gene expression by cDNA microarray analysis. Changes in gene expression with p-values less than 0.01 were considered to be statistically significant. The expression of 63 genes was changed by both MPEP and MTEP, with 58 genes down-regulated and 5 genes up-regulated. Quantitative PCR verified the magnitude and direction of change in expression of 9 of these genes (r(2)=0.556, p=0.017). Pathway analysis revealed that many of the biological processes altered by repeated MPEP and MTEP treatment were related to ATP synthesis, hydrolase activity, and signaling pathways associated with mitogen-activated protein kinase (MAPK). Our results demonstrate diverse effects of MPEP and MTEP gene expression in the frontal cortex, and these results may help elucidate the mechanisms by which these compounds produce beneficial effects in animal models of various disorders of the central nervous system.   PUBMED: 18346726
Ontological Annotations:
  • D011506: Proteins (Publication, NCBO Annotator)
  • D020411: Oligonucleotide Array Sequence Analysis (PubMed MeSH Annotations; Publication, NCBO Annotator)
  • D012640: Seizures (Publication, NCBO Annotator)
  • D000818: Animals (PubMed MeSH Annotations; Publication, NCBO Annotator)
  • D009422: Nervous System Diseases (Publication, NCBO Annotator)
  • D001008: Anxiety Disorders (Publication, NCBO Annotator)
  • D053263: Gene Regulatory Networks (PubMed MeSH Annotations)
  • D004364: Pharmaceutical Preparations (Publication, NCBO Annotator)
  • D007336: Insulin, NPH (Publication, NCBO Annotator)
  • D017470: Receptors, Glutamate (Publication, NCBO Annotator)
  • D006867: Hydrolases (Publication, NCBO Annotator; PubMed MeSH Annotations)
  • GO:0016787: hydrolase activity (Publication, NCBO Annotator)
  • D002385: Cataplexy (Publication, NCBO Annotator)
  • D004833: Epilepsy, Temporal Lobe (Publication, NCBO Annotator)
  • D014677: Pharmaceutical Vehicles (Publication, NCBO Annotator)
  • D004827: Epilepsy (Publication, NCBO Annotator)
  • D011494: Protein Kinases (Publication, NCBO Annotator)
  • D020133: Reverse Transcriptase Polymerase Chain Reaction (PubMed MeSH Annotations)
  • GO:0006754: ATP biosynthetic process (Publication, NCBO Annotator)
  • D018094: Receptors, Metabotropic Glutamate (PubMed MeSH Annotations; Publication, NCBO Annotator)
  • D055694: Biological Processes (Publication, NCBO Annotator)
  • D009949: Orientation (Publication, NCBO Annotator)
  • D019966: Substance-Related Disorders (Publication, NCBO Annotator)
  • D001007: Anxiety (Publication, NCBO Annotator)
  • D017346: Protein-Serine-Threonine Kinases (Publication, NCBO Annotator)
  • D020935: MAP Kinase Signaling System (PubMed MeSH Annotations)
  • D046228: Microarray Analysis (Publication, NCBO Annotator)
  • D004734: Energy Metabolism (PubMed MeSH Annotations)
  • D020937: Epilepsy, Partial, Sensory (Publication, NCBO Annotator)
  • D000303: Adrenal Cortex Diseases (Publication, NCBO Annotator)
  • D051381: Rats (PubMed MeSH Annotations; Publication, NCBO Annotator)
  • D003863: Depression (Publication, NCBO Annotator)
  • D009420: Nervous System (Publication, NCBO Annotator)
  • D007444: Inulin (Publication, NCBO Annotator)
  • MA:0000905: frontal cortex (Publication, NCBO Annotator)
  • GO:0023052: signaling (Publication, NCBO Annotator)
  • D023421: Models, Animal (Publication, NCBO Annotator)
  • D013672: Technology (Publication, NCBO Annotator)
  • D000437: Alcoholism (Publication, NCBO Annotator)
  • D013812: Therapeutics (Publication, NCBO Annotator)
  • GO:0010467: gene expression (Publication, NCBO Annotator)
  • D010770: Phosphotransferases (Publication, NCBO Annotator)
  • D014158: Transcription, Genetic (PubMed MeSH Annotations)
  • D009934: Organization and Administration (Publication, NCBO Annotator)
  • D018076: DNA, Complementary (Publication, NCBO Annotator)
  • D010300: Parkinson Disease (Publication, NCBO Annotator)
  • D017029: Epilepsy, Complex Partial (Publication, NCBO Annotator)
  • D000255: Adenosine Triphosphate (Publication, NCBO Annotator; PubMed MeSH Annotations)
  • MA:0000167: central nervous system (Publication, NCBO Annotator)
  • D020869: Gene Expression Profiling (PubMed MeSH Annotations)
  • D013844: Thiazoles (PubMed MeSH Annotations)
  • GO:0009058: biosynthetic process (Publication, NCBO Annotator)
  • D012380: Role (Publication, NCBO Annotator)
  • D000275: Adjustment Disorders (Publication, NCBO Annotator)
  • D016133: Polymerase Chain Reaction (Publication, NCBO Annotator)
  • D004832: Epilepsy, Absence (Publication, NCBO Annotator)
  • D002540: Cerebral Cortex (PubMed MeSH Annotations)
  • D003294: Seizures, Febrile (Publication, NCBO Annotator)
  • D016739: Behavior, Addictive (Publication, NCBO Annotator)
  • D003866: Depressive Disorder (Publication, NCBO Annotator)
  • D018698: Glutamic Acid (Publication, NCBO Annotator)
  • D017208: Rats, Wistar (Publication, NCBO Annotator; PubMed MeSH Annotations)
  • MA:0000017: sensory organ (Publication, NCBO Annotator)
  • MA:0000016: nervous system (Publication, NCBO Annotator)
  • D003865: Depressive Disorder, Major (Publication, NCBO Annotator)
  • D002490: Central Nervous System (Publication, NCBO Annotator)
  • D016000: Cluster Analysis (PubMed MeSH Annotations)
  • MP:0008912: nervous (Publication, NCBO Annotator)
  • D007274: Injections, Intraperitoneal (PubMed MeSH Annotations)
  • D018691: Excitatory Amino Acid Antagonists (PubMed MeSH Annotations)
  • D011725: Pyridines (PubMed MeSH Annotations)
  • D015203: Reproducibility of Results (PubMed MeSH Annotations)
  • MP:0002555: addiction (Publication, NCBO Annotator)

5 GeneSets from this Publication:


Select gene sets below using the check boxes, and then add them to a project using the pull down below. You can also create a project using the pull down. To view a set, click on the GeneSetID:GeneSet Description shown in green below.
Expand Tier III Rat 27 Genes GS1235: Transcriptional profiling of the rat frontal cortex following administration of the mGlu5 receptor antagonists MPEP and MTEP
Expand Tier I Rat DRG 8 Genes provisional GS86595: Table 3: Results of qPCR analysis of 9 selected genes from microarray findings. [DRG]
Expand Tier I Rat DRG 4 Genes provisional GS86668: Table 3: Results of qPCR analysis of 9 selected genes from microarray findings. [DRG]
Expand Tier I Rat DRG 27 Genes provisional GS87463: Table 1: List of genes changed by MPEP and MTEP (Whole Table) [DRG]
Expand Tier I Rat DRG 26 Genes provisional GS87464: Table 1: List of genes changed by MPEP and MTEP (Whole Table) [DRG]